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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F13%3A00396828%21RIV14-AV0-60077344
rdf:type
n8:Vysledek skos:Concept
dcterms:description
Aims: To evaluate the possibility latent microsporidiosis, efficacy or albendazole, and reactivation, the authors monitored the course of E. cuniculi infection in immunocompetent BALB/c mice and immunodeficient SCID mice using molecular methods. Methods: Mice were per orally infected with 10(7) spores of E. cuniculi. Selected groups were treated with albendazole, re-infected or chemically immunosuppressed by dexamethasone. The presence of microsporidia in the host's organs and feces were determined using PCR methods. Changes in numbers of lymphocytes in blood and in spleen after induction of immunosuppression were confirmed using flow cytometry analysis. Results: Whereas E. cuniculi caused lethal microsporidiosis in SCID mice, the infection in BABL/c mice remained asymptomatic despite parasite dissemination into many organs during the acute infection phase. Albendazole treatment led to microsporidia elimination from organs in BALB/c mice. In SCID mice, however, only a temporary reduction in number of affected organs was observed and infection re-established post-treatment. Dexamethasone treatment resulted in a chronic microsporidia infection disseminating into most organs in BALB/c mice. Although the presence of E. cuniculi in organs of albendazole- treated mice was undetectable by PCR, it was striking that infection was reactivated by immunosuppression treatment. Conclusion: Our results demonstrated that microsporidia can successfully survive in organs of immunocompetent hosts and are able to reactivate from undetectable levels and spread within these hosts after induction of immunosuppression. These findings stress the danger of latent microsporidiosis as a life-threatening risk factor especially for individuals undergoing chemotherapy and in transplant recipients of organs originating from infected donors. Aims: To evaluate the possibility latent microsporidiosis, efficacy or albendazole, and reactivation, the authors monitored the course of E. cuniculi infection in immunocompetent BALB/c mice and immunodeficient SCID mice using molecular methods. Methods: Mice were per orally infected with 10(7) spores of E. cuniculi. Selected groups were treated with albendazole, re-infected or chemically immunosuppressed by dexamethasone. The presence of microsporidia in the host's organs and feces were determined using PCR methods. Changes in numbers of lymphocytes in blood and in spleen after induction of immunosuppression were confirmed using flow cytometry analysis. Results: Whereas E. cuniculi caused lethal microsporidiosis in SCID mice, the infection in BABL/c mice remained asymptomatic despite parasite dissemination into many organs during the acute infection phase. Albendazole treatment led to microsporidia elimination from organs in BALB/c mice. In SCID mice, however, only a temporary reduction in number of affected organs was observed and infection re-established post-treatment. Dexamethasone treatment resulted in a chronic microsporidia infection disseminating into most organs in BALB/c mice. Although the presence of E. cuniculi in organs of albendazole- treated mice was undetectable by PCR, it was striking that infection was reactivated by immunosuppression treatment. Conclusion: Our results demonstrated that microsporidia can successfully survive in organs of immunocompetent hosts and are able to reactivate from undetectable levels and spread within these hosts after induction of immunosuppression. These findings stress the danger of latent microsporidiosis as a life-threatening risk factor especially for individuals undergoing chemotherapy and in transplant recipients of organs originating from infected donors.
dcterms:title
Latent Microsporidiosis Caused by Encephalitozoon cuniculi in Immunocompetent Hosts: A Murine Model Demonstrating the Ineffectiveness of the Immune System and Treatment with Albendazole Latent Microsporidiosis Caused by Encephalitozoon cuniculi in Immunocompetent Hosts: A Murine Model Demonstrating the Ineffectiveness of the Immune System and Treatment with Albendazole
skos:prefLabel
Latent Microsporidiosis Caused by Encephalitozoon cuniculi in Immunocompetent Hosts: A Murine Model Demonstrating the Ineffectiveness of the Immune System and Treatment with Albendazole Latent Microsporidiosis Caused by Encephalitozoon cuniculi in Immunocompetent Hosts: A Murine Model Demonstrating the Ineffectiveness of the Immune System and Treatment with Albendazole
skos:notation
RIV/60077344:_____/13:00396828!RIV14-AV0-60077344
n8:predkladatel
n18:ico%3A60077344
n4:aktivita
n13:P n13:I
n4:aktivity
I, P(GAP505/11/1163), P(LH11061)
n4:cisloPeriodika
4
n4:dodaniDat
n19:2014
n4:domaciTvurceVysledku
n10:4228677 n10:8111618 n10:8524777 n10:8810052
n4:druhVysledku
n6:J
n4:duvernostUdaju
n16:S
n4:entitaPredkladatele
n9:predkladatel
n4:idSjednocenehoVysledku
84394
n4:idVysledku
RIV/60077344:_____/13:00396828
n4:jazykVysledku
n7:eng
n4:klicovaSlova
immunodeficiency virus infection; scide mice; Enterocytozoon bieneusi; patient; lymphocytes; epidemiology; intestinals; confirmation; macrophages; antibodies
n4:klicoveSlovo
n5:antibodies n5:Enterocytozoon%20bieneusi n5:intestinals n5:patient n5:lymphocytes n5:epidemiology n5:confirmation n5:macrophages n5:immunodeficiency%20virus%20infection n5:scide%20mice
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[58548F3B082C]
n4:nazevZdroje
PLoS ONE
n4:obor
n15:EC
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
4
n4:projekt
n14:LH11061 n14:GAP505%2F11%2F1163
n4:rokUplatneniVysledku
n19:2013
n4:svazekPeriodika
8
n4:tvurceVysledku
Kváč, Martin Sak, Bohumil Kotková, Michaela Květoňová, Dana
n4:wos
000317383200031
s:issn
1932-6203
s:numberOfPages
7
n11:doi
10.1371/journal.pone.0060941