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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F13%3A00395286%21RIV14-AV0-60077344
rdf:type
skos:Concept n7:Vysledek
rdfs:seeAlso
http://link.springer.com/content/pdf/10.1007%2Fs12010-013-0325-9.pdf
dcterms:description
Several recombinant derivatives of silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were highly active against subtilisin and proteinase K. A construct of the gene was introduced into Agrobacterium tumefaciens that was then used for genetic transformation of the potato variety Velox. In vitro tests showed increased resistance to the late blight Phytophthora infestans in several transformed lines. No effect was seen on biological characteristics of Spodoptera littoralis indicating that the transformed potatoes may be harmless to non-target organisms. Several recombinant derivatives of silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were highly active against subtilisin and proteinase K. A construct of the gene was introduced into Agrobacterium tumefaciens that was then used for genetic transformation of the potato variety Velox. In vitro tests showed increased resistance to the late blight Phytophthora infestans in several transformed lines. No effect was seen on biological characteristics of Spodoptera littoralis indicating that the transformed potatoes may be harmless to non-target organisms.
dcterms:title
Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato
skos:prefLabel
Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato
skos:notation
RIV/60077344:_____/13:00395286!RIV14-AV0-60077344
n7:predkladatel
n8:ico%3A60077344
n3:aktivita
n4:P n4:V n4:I
n3:aktivity
I, P(QI91A229), V
n3:cisloPeriodika
1
n3:dodaniDat
n19:2014
n3:domaciTvurceVysledku
Vinokurov, Konstantin n12:5729629 n12:4230930 n12:3400603 Kludkiewicz, Barbara
n3:druhVysledku
n9:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
100505
n3:idVysledku
RIV/60077344:_____/13:00395286
n3:jazykVysledku
n16:eng
n3:klicovaSlova
genetically modified organism; silk protease inhibitor; protease
n3:klicoveSlovo
n15:silk%20protease%20inhibitor n15:protease n15:genetically%20modified%20organism
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[41B7A2675E25]
n3:nazevZdroje
Applied Biochemistry and Biotechnology
n3:obor
n17:ED
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
8
n3:projekt
n10:QI91A229
n3:rokUplatneniVysledku
n19:2013
n3:svazekPeriodika
171
n3:tvurceVysledku
Kodrík, Dalibor Kludkiewicz, Barbara Svobodová, Z. Horáčková, V. Skoková Habuštová, Oxana Sehnal, František Vinokurov, Konstantin Navrátil, Oldřich
n3:wos
000324109800018
s:issn
0273-2289
s:numberOfPages
16
n11:doi
10.1007/s12010-013-0325-9