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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F13%3A00395153%21RIV14-AV0-60077344
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Symptoms of unknown aetiology on Rhododendron hybridum were observed in the Czech Republic in 2010. The infected plant had malformed leaves, with irregular shaped edges, mosaic, leaf tip necrosis and multiple axillary shoots with smaller leaves. Transmission electron microscopy showed phytoplasma-like bodies in phloem cells of the symptomatic plant. Phytoplasma presence was confirmed by PCR using phytoplasma-specific, universal and group-specific primer pairs. RFLP analysis of 16S rDNA enabled classification of the detected phytoplasma into the aster yellows subgroup I-C. Sequence analysis of the 16S-23S ribosomal operon of the amplified phytoplasma genome from the infected rhododendron plant (1724bp) confirmed the closest relationship with the Czech Echinacea purpurea phyllody phytoplasma. These data suggest Rhododendron hybridum is a new host for the aster yellows phytoplasma subgroup 16SrI-C in the Czech Republic and worldwide. Symptoms of unknown aetiology on Rhododendron hybridum were observed in the Czech Republic in 2010. The infected plant had malformed leaves, with irregular shaped edges, mosaic, leaf tip necrosis and multiple axillary shoots with smaller leaves. Transmission electron microscopy showed phytoplasma-like bodies in phloem cells of the symptomatic plant. Phytoplasma presence was confirmed by PCR using phytoplasma-specific, universal and group-specific primer pairs. RFLP analysis of 16S rDNA enabled classification of the detected phytoplasma into the aster yellows subgroup I-C. Sequence analysis of the 16S-23S ribosomal operon of the amplified phytoplasma genome from the infected rhododendron plant (1724bp) confirmed the closest relationship with the Czech Echinacea purpurea phyllody phytoplasma. These data suggest Rhododendron hybridum is a new host for the aster yellows phytoplasma subgroup 16SrI-C in the Czech Republic and worldwide.
dcterms:title
Aster Yellows Subgroup 16SrI-C Phytoplasma in Rhododendron hybridum Aster Yellows Subgroup 16SrI-C Phytoplasma in Rhododendron hybridum
skos:prefLabel
Aster Yellows Subgroup 16SrI-C Phytoplasma in Rhododendron hybridum Aster Yellows Subgroup 16SrI-C Phytoplasma in Rhododendron hybridum
skos:notation
RIV/60077344:_____/13:00395153!RIV14-AV0-60077344
n18:predkladatel
n19:ico%3A60077344
n3:aktivita
n7:P n7:I
n3:aktivity
I, P(LD12074)
n3:cisloPeriodika
7-8
n3:dodaniDat
n8:2014
n3:domaciTvurceVysledku
n5:3020630 n5:9037098 n5:2845768
n3:druhVysledku
n10:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
62364
n3:idVysledku
RIV/60077344:_____/13:00395153
n3:jazykVysledku
n4:eng
n3:klicovaSlova
electron microscopy; PCR; phytoplasma
n3:klicoveSlovo
n12:electron%20microscopy n12:PCR n12:phytoplasma
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[C676F7231477]
n3:nazevZdroje
Journal of Phytopathology - Phytopathologische Zeitschrift
n3:obor
n13:EE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n11:LD12074
n3:rokUplatneniVysledku
n8:2013
n3:svazekPeriodika
161
n3:tvurceVysledku
Přibylová, Jaroslava Fránová, Jana Špak, Josef
n3:wos
000321507600019
s:issn
0931-1785
s:numberOfPages
4
n17:doi
10.1111/jph.12099