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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F13%3A00392960%21RIV14-AV0-60077344
rdf:type
skos:Concept n14:Vysledek
dcterms:description
A transformation vector harboring the marker gene aadA conferring spectinomycin resistance and the fusion E7GGG/gus gene of interest was employed for the transformation of tobacco cpDNA via biolistics. The gene of interest consisted of the oncogenic E7GGG gene from human papillomavirus strain 16 fused with the reporter gus gene. We identified 6 plants harboring the E7GGG/gus gene only, where the marker gene was eliminated by homologous recombination, one plant with both transgenes, and one with the aadA gene only. RT-PCR showed the presence of the respective mRNAs in all 8 plants analyzed but Western blot proved that only the GUS part of the fusion E7GGG/GUS protein was present in all plants harboring the fusion gene. A transformation vector harboring the marker gene aadA conferring spectinomycin resistance and the fusion E7GGG/gus gene of interest was employed for the transformation of tobacco cpDNA via biolistics. The gene of interest consisted of the oncogenic E7GGG gene from human papillomavirus strain 16 fused with the reporter gus gene. We identified 6 plants harboring the E7GGG/gus gene only, where the marker gene was eliminated by homologous recombination, one plant with both transgenes, and one with the aadA gene only. RT-PCR showed the presence of the respective mRNAs in all 8 plants analyzed but Western blot proved that only the GUS part of the fusion E7GGG/GUS protein was present in all plants harboring the fusion gene.
dcterms:title
Transformation of tobacco cpDNA with fusion E7GGG/GUS gene and homologous recombination mediated elimination of the marker gene Transformation of tobacco cpDNA with fusion E7GGG/GUS gene and homologous recombination mediated elimination of the marker gene
skos:prefLabel
Transformation of tobacco cpDNA with fusion E7GGG/GUS gene and homologous recombination mediated elimination of the marker gene Transformation of tobacco cpDNA with fusion E7GGG/GUS gene and homologous recombination mediated elimination of the marker gene
skos:notation
RIV/60077344:_____/13:00392960!RIV14-AV0-60077344
n14:predkladatel
n15:ico%3A60077344
n3:aktivita
n5:P n5:V n5:I
n3:aktivity
I, P(IAA500960903), V
n3:cisloPeriodika
2
n3:dodaniDat
n6:2014
n3:domaciTvurceVysledku
n8:8199655 n8:4095154 n8:2637413
n3:druhVysledku
n9:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
111583
n3:idVysledku
RIV/60077344:_____/13:00392960
n3:jazykVysledku
n13:eng
n3:klicovaSlova
E7GGG oncogene; chloroplast transformation; marker-free plant; homologous recombination
n3:klicoveSlovo
n11:chloroplast%20transformation n11:marker-free%20plant n11:E7GGG%20oncogene n11:homologous%20recombination
n3:kodStatuVydavatele
BG - Bulharská republika
n3:kontrolniKodProRIV
[451F2A1C3D16]
n3:nazevZdroje
Biotechnology & Biotechnological Equipment
n3:obor
n16:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
5
n3:projekt
n19:IAA500960903
n3:rokUplatneniVysledku
n6:2013
n3:svazekPeriodika
27
n3:tvurceVysledku
Niedermeierová, Hana Vlasák, Josef Ryba, Š. Bříza, Jindřich Ludvíková, V.
n3:wos
000317997400005
s:issn
1310-2818
s:numberOfPages
5
n4:doi
10.5504/BBEQ.2012.0141