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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F12%3A00391203%21RIV13-GA0-60077344
rdf:type
n3:Vysledek skos:Concept
dcterms:description
Bacillus thuringiensis Cry3A gene was redesigned for high expression in Norwegian spruce and the sequence was slightly modified to allow for simple N- and C- terminal deletions and domain II loop 1 exchange for synthetic oligos. Modified Cry3A toxins from 13 variants of the synthetic gene were expressed in Escherichia coli BL21 and their toxicity on spruce bark beetle larvae was tested using spruce bark sandwiches. Mutant toxins with N-terminal deletion and loop 1 duplication showed increased toxicity. Bacillus thuringiensis Cry3A gene was redesigned for high expression in Norwegian spruce and the sequence was slightly modified to allow for simple N- and C- terminal deletions and domain II loop 1 exchange for synthetic oligos. Modified Cry3A toxins from 13 variants of the synthetic gene were expressed in Escherichia coli BL21 and their toxicity on spruce bark beetle larvae was tested using spruce bark sandwiches. Mutant toxins with N-terminal deletion and loop 1 duplication showed increased toxicity.
dcterms:title
Cry3A ä-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop Cry3A ä-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop
skos:prefLabel
Cry3A ä-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop Cry3A ä-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop
skos:notation
RIV/60077344:_____/12:00391203!RIV13-GA0-60077344
n3:predkladatel
n20:ico%3A60077344
n4:aktivita
n16:P n16:I n16:Z
n4:aktivity
I, P(GAP502/11/1471), P(QH71290), Z(AV0Z50510513)
n4:cisloPeriodika
86
n4:dodaniDat
n6:2013
n4:domaciTvurceVysledku
n12:9784160 n12:2637413 n12:8199655
n4:druhVysledku
n14:J
n4:duvernostUdaju
n19:S
n4:entitaPredkladatele
n5:predkladatel
n4:idSjednocenehoVysledku
129138
n4:idVysledku
RIV/60077344:_____/12:00391203
n4:jazykVysledku
n11:eng
n4:klicovaSlova
Bacillus thuringiensis; Ips typographus; Picea abies
n4:klicoveSlovo
n7:Bacillus%20thuringiensis n7:Ips%20typographus n7:Picea%20abies
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[1F5BD3828B8A]
n4:nazevZdroje
African Journal of Biotechnology
n4:obor
n15:EI
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
6
n4:projekt
n10:QH71290 n10:GAP502%2F11%2F1471
n4:rokUplatneniVysledku
n6:2012
n4:svazekPeriodika
11
n4:tvurceVysledku
Malá, J. Modlinger, R. Pavingerová, Daniela Bříza, Jindřich Knížek, M. Vlasák, Josef
n4:zamer
n17:AV0Z50510513
s:issn
1684-5315
s:numberOfPages
5
n18:doi
10.5897/AJB11.3806