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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F12%3A00388090%21RIV14-AV0-60077344
rdf:type
n11:Vysledek skos:Concept
dcterms:description
Measurements of microbiological parameters are not currently widely used for protection, monitoring and preservation of caves although they indicate very well the recent human impact. Here we present a commercially available microbiological kit for cave ecologists, the RIDA COUNT test kit (R-Biopharm AG, Germany), as a supplementary tool for research and show examples. Simultaneously, lists of microbial indicator groups and cave microhabitats, where this methodology may be appllied, are presented. Indicators include certain clinically important human-associated microbes such as Escherichia coli, Salmonella spp., Staphylococcus aureus that are easy to quantify with basic cultivation methodology. Relatively higher bacterial counts compared to yeast and moulds on RIDA COUNT test plates indicate recent and pronounced human impact. Swab samples allow detection of gradients of surface microbial colonization and determination of the microbial load on footprints and fingerprints in caves. In our tests, RIDA COUNT plates for enumeration of yeast and moulds revealed a similar microbial load between unwashed caving boots and human fingerprints on a metal fence. Similarly, total bacterial counts were comparable between these two surfaces, 5,890 CFU/100 cm2 for unwashed boots and 4,340 CFU/100 cm2 for fingerprints on metal fence. Bacterial counts on walking surfaces in show caves can exceed 10,000 CFU/100 cm2 (Postojna Cave). These examples show that quantification of microbial indicator groups revealed increased microbial load and possible biohazard in the underground. This procedure may be widely adopted as a part of a regular monitoring programme in caves. Measurements of microbiological parameters are not currently widely used for protection, monitoring and preservation of caves although they indicate very well the recent human impact. Here we present a commercially available microbiological kit for cave ecologists, the RIDA COUNT test kit (R-Biopharm AG, Germany), as a supplementary tool for research and show examples. Simultaneously, lists of microbial indicator groups and cave microhabitats, where this methodology may be appllied, are presented. Indicators include certain clinically important human-associated microbes such as Escherichia coli, Salmonella spp., Staphylococcus aureus that are easy to quantify with basic cultivation methodology. Relatively higher bacterial counts compared to yeast and moulds on RIDA COUNT test plates indicate recent and pronounced human impact. Swab samples allow detection of gradients of surface microbial colonization and determination of the microbial load on footprints and fingerprints in caves. In our tests, RIDA COUNT plates for enumeration of yeast and moulds revealed a similar microbial load between unwashed caving boots and human fingerprints on a metal fence. Similarly, total bacterial counts were comparable between these two surfaces, 5,890 CFU/100 cm2 for unwashed boots and 4,340 CFU/100 cm2 for fingerprints on metal fence. Bacterial counts on walking surfaces in show caves can exceed 10,000 CFU/100 cm2 (Postojna Cave). These examples show that quantification of microbial indicator groups revealed increased microbial load and possible biohazard in the underground. This procedure may be widely adopted as a part of a regular monitoring programme in caves.
dcterms:title
Monitoring of microbial indicator groups in caves through the use of RIDA COUNT kits Monitoring of microbial indicator groups in caves through the use of RIDA COUNT kits
skos:prefLabel
Monitoring of microbial indicator groups in caves through the use of RIDA COUNT kits Monitoring of microbial indicator groups in caves through the use of RIDA COUNT kits
skos:notation
RIV/60077344:_____/12:00388090!RIV14-AV0-60077344
n11:predkladatel
n13:ico%3A60077344
n3:aktivita
n5:P n5:I
n3:aktivity
I, P(LC06066)
n3:cisloPeriodika
2/3
n3:dodaniDat
n4:2014
n3:domaciTvurceVysledku
n10:1850261 n10:7237243
n3:druhVysledku
n17:J
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
151660
n3:idVysledku
RIV/60077344:_____/12:00388090
n3:jazykVysledku
n15:eng
n3:klicovaSlova
caves; microorganisms; monitoring; human impact; water quality
n3:klicoveSlovo
n7:caves n7:water%20quality n7:human%20impact n7:monitoring n7:microorganisms
n3:kodStatuVydavatele
SI - Slovinská republika
n3:kontrolniKodProRIV
[8C2C39C38837]
n3:nazevZdroje
Acta carsologica
n3:obor
n18:EE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:projekt
n8:LC06066
n3:rokUplatneniVysledku
n4:2012
n3:svazekPeriodika
41
n3:tvurceVysledku
Chroňáková, Alica Mulec, J. Krištůfek, Václav
n3:wos
000315009800009
s:issn
0583-6050
s:numberOfPages
9