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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F12%3A00387779%21RIV13-AV0-60077344
rdf:type
n10:Vysledek skos:Concept
rdfs:seeAlso
http://www.parasitesandvectors.com/content/5/1/219
dcterms:description
Background: In Central Asian foci of zoonotic cutaneous leishmaniases, mixed infections of Leishmania turanica and L. major have been found in a reservoir host (the great gerbil, Rhombomys opimus) as well as in the sand fly vector Phlebotomus papatasi, but hybrids between these two Leishmania species have never been reported. In addition, the role of sand fly species other than P. papatasi in L. turanica circulation is not clear. Methods: In this work we compared the development of L. turanica in three sand fly species belonging to different subgenera. In addition, we studied experimental co-infections of sand flies by both Leishmania species using GFP transfected L. turanica (MRHO/MN/08/BZ18(GFP+)) and RFP transfected L. major (WHOM/IR/-/173-DsRED(RFP+)). The possibility of Leishmania genetic exchange during the vectorial part of the life cycle was studied using flow cytometry combined with immunofluorescent microscopy. Results: Late-stage infections of L. turanica with frequent colonization of the stomodeal valve were observed in the specific vector P. (Phlebotomus) papatasi and in the permissive vector P. (Adlerius) arabicus. On the other hand, in P. sergenti (the specific vector of L. tropica), L. turanica promatigotes were present only until the defecation of bloodmeal remnants. In their natural vector P. papatasi, L. turanica and L. major developed similarly, and the spatiotemporal dynamics of localization in the sand fly gut was the same for both leishmania species. Fluorescence microscopy in combination with FACS analyses did not detect any L. major / L. turanica hybrids in the experimental co-infection of P. papatasi and P. duboscqi. Conclusion: Our data provide new insight into the development of different leishmania parasite species during a mixed infection in the sand fly gut. Despite the fact that both Leishmania species developed well in P. papatasi and P. duboscqi and did not outcompete each other, no genetic exchange was found. Background: In Central Asian foci of zoonotic cutaneous leishmaniases, mixed infections of Leishmania turanica and L. major have been found in a reservoir host (the great gerbil, Rhombomys opimus) as well as in the sand fly vector Phlebotomus papatasi, but hybrids between these two Leishmania species have never been reported. In addition, the role of sand fly species other than P. papatasi in L. turanica circulation is not clear. Methods: In this work we compared the development of L. turanica in three sand fly species belonging to different subgenera. In addition, we studied experimental co-infections of sand flies by both Leishmania species using GFP transfected L. turanica (MRHO/MN/08/BZ18(GFP+)) and RFP transfected L. major (WHOM/IR/-/173-DsRED(RFP+)). The possibility of Leishmania genetic exchange during the vectorial part of the life cycle was studied using flow cytometry combined with immunofluorescent microscopy. Results: Late-stage infections of L. turanica with frequent colonization of the stomodeal valve were observed in the specific vector P. (Phlebotomus) papatasi and in the permissive vector P. (Adlerius) arabicus. On the other hand, in P. sergenti (the specific vector of L. tropica), L. turanica promatigotes were present only until the defecation of bloodmeal remnants. In their natural vector P. papatasi, L. turanica and L. major developed similarly, and the spatiotemporal dynamics of localization in the sand fly gut was the same for both leishmania species. Fluorescence microscopy in combination with FACS analyses did not detect any L. major / L. turanica hybrids in the experimental co-infection of P. papatasi and P. duboscqi. Conclusion: Our data provide new insight into the development of different leishmania parasite species during a mixed infection in the sand fly gut. Despite the fact that both Leishmania species developed well in P. papatasi and P. duboscqi and did not outcompete each other, no genetic exchange was found.
dcterms:title
The development of Leishmania turanica in sand flies and competition with L. major The development of Leishmania turanica in sand flies and competition with L. major
skos:prefLabel
The development of Leishmania turanica in sand flies and competition with L. major The development of Leishmania turanica in sand flies and competition with L. major
skos:notation
RIV/60077344:_____/12:00387779!RIV13-AV0-60077344
n10:predkladatel
n12:ico%3A60077344
n3:aktivita
n8:Z n8:P n8:I
n3:aktivity
I, P(7E11055), Z(MSM0021620828)
n3:cisloPeriodika
OCT 2
n3:dodaniDat
n5:2013
n3:domaciTvurceVysledku
n11:3804399 n11:8635137
n3:druhVysledku
n14:J
n3:duvernostUdaju
n20:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
130801
n3:idVysledku
RIV/60077344:_____/12:00387779
n3:jazykVysledku
n15:eng
n3:klicovaSlova
Leishmania turanica; L. major; mixed infections; competition; genetic exchange; vector competence; Phlebotomus
n3:klicoveSlovo
n9:vector%20competence n9:genetic%20exchange n9:mixed%20infections n9:L.%20major n9:competition n9:Leishmania%20turanica n9:Phlebotomus
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[6B16D9CB31AA]
n3:nazevZdroje
Parasites Vectors
n3:obor
n18:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
11
n3:projekt
n17:7E11055
n3:rokUplatneniVysledku
n5:2012
n3:svazekPeriodika
5
n3:tvurceVysledku
Kreisinger, J. Chajbullinova, A. Seblova, V. Gantuya, S. Kvapilová, K. Matsumoto, Y. Jirků, Milan Votýpka, Jan Sádlová, J. Volf, P. Sanjoba, C.
n3:wos
000310460800001
n3:zamer
n21:MSM0021620828
s:issn
1756-3305
s:numberOfPages
8
n19:doi
10.1186/1756-3305-5-219