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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F12%3A00386218%21RIV13-AV0-60077344
rdf:type
n15:Vysledek skos:Concept
rdfs:seeAlso
http://onlinelibrary.wiley.com/doi/10.1002/cmmi.1477/full
dcterms:description
Labeling of pancreatic islets with superparamagnetic iron oxide (SPIO) nanoparticles enables their post-transplant monitoring by magnetic resonance imaging (MRI). Although the nanoparticles are incorporated into islet cells in culture, little is known about their fate in vivo. We studied the morphology of labeled islets after transplantation, aiming to identify the MRI contrast particles and their relationship to transplantation outcomes. Rat islets labeled with the ferucarbotran were transplanted into the liver or under the kidney capsule of syngeneic and allogeneic rats. After in vivo MRI, morphology was studied by light, fluorescence and transmission electron microscopy. Morphology of syngeneic islets transplanted beneath the kidney capsule vs into the liver was similar. Iron particles were almost completely eliminated from the endocrine cells and remained located in host-derived macrophages surrounding the vital islets for the entire study period. In the allogeneic model, islets lost their function and were completely rejected within nine days following transplantation in both transplant models. However, intercellular transport of the SPIO particles and subsequent MRI findings was different in the liver and kidney. In the liver, the decreasing number of islet-related MRI spots corresponded with clearance of iron particles in rejected islets; in contrast, with renal transplants extensive iron deposits with a high effect on MRI signal persisted in phagocytic cells beneath the capsule. We conclude that MRI detection of the iron contrast agent correlates with islet survival and function in islet transplantation into the liver, while it does not correlate in the case of transplantation beneath the renal capsule. Labeling of pancreatic islets with superparamagnetic iron oxide (SPIO) nanoparticles enables their post-transplant monitoring by magnetic resonance imaging (MRI). Although the nanoparticles are incorporated into islet cells in culture, little is known about their fate in vivo. We studied the morphology of labeled islets after transplantation, aiming to identify the MRI contrast particles and their relationship to transplantation outcomes. Rat islets labeled with the ferucarbotran were transplanted into the liver or under the kidney capsule of syngeneic and allogeneic rats. After in vivo MRI, morphology was studied by light, fluorescence and transmission electron microscopy. Morphology of syngeneic islets transplanted beneath the kidney capsule vs into the liver was similar. Iron particles were almost completely eliminated from the endocrine cells and remained located in host-derived macrophages surrounding the vital islets for the entire study period. In the allogeneic model, islets lost their function and were completely rejected within nine days following transplantation in both transplant models. However, intercellular transport of the SPIO particles and subsequent MRI findings was different in the liver and kidney. In the liver, the decreasing number of islet-related MRI spots corresponded with clearance of iron particles in rejected islets; in contrast, with renal transplants extensive iron deposits with a high effect on MRI signal persisted in phagocytic cells beneath the capsule. We conclude that MRI detection of the iron contrast agent correlates with islet survival and function in islet transplantation into the liver, while it does not correlate in the case of transplantation beneath the renal capsule.
dcterms:title
Processing of superparamagnetic iron contrast agent ferucarbotran in transplanted pancreatic islets Processing of superparamagnetic iron contrast agent ferucarbotran in transplanted pancreatic islets
skos:prefLabel
Processing of superparamagnetic iron contrast agent ferucarbotran in transplanted pancreatic islets Processing of superparamagnetic iron contrast agent ferucarbotran in transplanted pancreatic islets
skos:notation
RIV/60077344:_____/12:00386218!RIV13-AV0-60077344
n15:predkladatel
n20:ico%3A60077344
n3:aktivita
n5:Z n5:I
n3:aktivity
I, Z(AV0Z60220518)
n3:cisloPeriodika
6
n3:dodaniDat
n9:2013
n3:domaciTvurceVysledku
n19:8966338
n3:druhVysledku
n10:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
162418
n3:idVysledku
RIV/60077344:_____/12:00386218
n3:jazykVysledku
n12:eng
n3:klicovaSlova
magnetic resonance imaging; pancreatic islets; transplantation; superparamagnetic iron oxide nanoparticles; ferucarbotran; β cells; diabetes; immunohistochemistry; transmission electron microscopy
n3:klicoveSlovo
n7:magnetic%20resonance%20imaging n7:%CE%B2%20cells n7:superparamagnetic%20iron%20oxide%20nanoparticles n7:immunohistochemistry n7:ferucarbotran n7:transmission%20electron%20microscopy n7:transplantation n7:pancreatic%20islets n7:diabetes
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[612BEBF30304]
n3:nazevZdroje
Contrast Media & Molecular Imaging
n3:obor
n13:CE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
7
n3:rokUplatneniVysledku
n9:2012
n3:svazekPeriodika
7
n3:tvurceVysledku
Jirák, D. Vancová, Marie Berková, Z. Herynek, V. Zacharovová, K. Dovolilová, E. Saudek, F.
n3:wos
000308940900002
n3:zamer
n6:AV0Z60220518
s:issn
1555-4309
s:numberOfPages
9
n17:doi
10.1002/cmmi.1477