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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F12%3A00381542%21RIV13-GA0-60077344
rdf:type
n6:Vysledek skos:Concept
dcterms:description
Metabolic engineering of the flavonoid pathway may be used for modifying nutritional, pharmaceutical and ornamental properties of plants. The PAP1 (AtMYB75) is a R2R3 MYB family member and stimulates the expression of flavonoid pathway genes in Arabidopsis thaliana . Previously, PAP1 was introduced into Humulus lupulus plants by Agrobacterium-mediated genetic transformation. Employing quantitative PCR, we show that each transgenic plant harbors one copy of PAP1. The expression of the structural genes CHS_H1, CHI, and F3'H, essential for the biosynthesis of flavonoids, was elevated in PAP1 transgenic plants compared to the wildtype hop. Chemometric methods were successfully used to discriminate between wildtype and transgenic plants expressing the transcription factor PAP1. Thus, transcription factor PAP1 activated the expression of these three genes in transgenic plants, opening the possibility for improving the content of pharmaceutically important secondary metabolites in hop. Metabolic engineering of the flavonoid pathway may be used for modifying nutritional, pharmaceutical and ornamental properties of plants. The PAP1 (AtMYB75) is a R2R3 MYB family member and stimulates the expression of flavonoid pathway genes in Arabidopsis thaliana . Previously, PAP1 was introduced into Humulus lupulus plants by Agrobacterium-mediated genetic transformation. Employing quantitative PCR, we show that each transgenic plant harbors one copy of PAP1. The expression of the structural genes CHS_H1, CHI, and F3'H, essential for the biosynthesis of flavonoids, was elevated in PAP1 transgenic plants compared to the wildtype hop. Chemometric methods were successfully used to discriminate between wildtype and transgenic plants expressing the transcription factor PAP1. Thus, transcription factor PAP1 activated the expression of these three genes in transgenic plants, opening the possibility for improving the content of pharmaceutically important secondary metabolites in hop.
dcterms:title
The transcription factor AtMYB75/PAP1 regulates the expression of flavonoid biosynthesis genes in transgenic hop (Humulus lupulus L.) The transcription factor AtMYB75/PAP1 regulates the expression of flavonoid biosynthesis genes in transgenic hop (Humulus lupulus L.)
skos:prefLabel
The transcription factor AtMYB75/PAP1 regulates the expression of flavonoid biosynthesis genes in transgenic hop (Humulus lupulus L.) The transcription factor AtMYB75/PAP1 regulates the expression of flavonoid biosynthesis genes in transgenic hop (Humulus lupulus L.)
skos:notation
RIV/60077344:_____/12:00381542!RIV13-GA0-60077344
n6:predkladatel
n18:ico%3A60077344
n3:aktivita
n9:Z n9:P n9:I
n3:aktivity
I, P(GA521/08/0740), Z(AV0Z50510513)
n3:cisloPeriodika
7-8
n3:dodaniDat
n12:2013
n3:domaciTvurceVysledku
n14:7309430
n3:druhVysledku
n11:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
174851
n3:idVysledku
RIV/60077344:_____/12:00381542
n3:jazykVysledku
n13:eng
n3:klicovaSlova
metabolic engineering; Humulus lupulus L.; transcription factors; flavonoid biosynthesis
n3:klicoveSlovo
n10:flavonoid%20biosynthesis n10:metabolic%20engineering n10:transcription%20factors n10:Humulus%20lupulus%20L.
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[9876BC213619]
n3:nazevZdroje
BrewingScience
n3:obor
n7:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:projekt
n16:GA521%2F08%2F0740
n3:rokUplatneniVysledku
n12:2012
n3:svazekPeriodika
65
n3:tvurceVysledku
Gatica-Arias, A. Weber, G. Häntzschel, K. R. Farag, M. A. Matoušek, Jaroslav
n3:zamer
n17:AV0Z50510513
s:issn
1866-5195
s:numberOfPages
9