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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F12%3A00369909%21RIV12-MSM-60077344
rdf:type
skos:Concept n12:Vysledek
dcterms:description
Gas chromatography (GC) is a commonly used technique in amino acid analysis (AAA). However, one of the requirements of the application of GC for AAA is a need for the polar analytes to be converted into their volatile, thermally stable derivatives. In the last two decades, alkyl chloroformates have become attractive derivatization reagents. The reagents react immediately with most amino acid functional groups in aqueous matrices and the process can easily be coupled with liquid–liquid extraction of the resulting less-polar derivatives into immiscible organic phase. Here, we describe a simple protocol for in situ derivatization of amino acids with heptafluorobutyl chloroformate followed by subsequent chiral as well as nonchiral GC/mass spectrometric analysis on a respective nonpolar fused silica and an enantioselective Chirasil-Val capillary column. Gas chromatography (GC) is a commonly used technique in amino acid analysis (AAA). However, one of the requirements of the application of GC for AAA is a need for the polar analytes to be converted into their volatile, thermally stable derivatives. In the last two decades, alkyl chloroformates have become attractive derivatization reagents. The reagents react immediately with most amino acid functional groups in aqueous matrices and the process can easily be coupled with liquid–liquid extraction of the resulting less-polar derivatives into immiscible organic phase. Here, we describe a simple protocol for in situ derivatization of amino acids with heptafluorobutyl chloroformate followed by subsequent chiral as well as nonchiral GC/mass spectrometric analysis on a respective nonpolar fused silica and an enantioselective Chirasil-Val capillary column.
dcterms:title
Heptafluorobutyl chloroformate - based sample preparation protocol for chiral and nonchiral amino acid analysis by gas chromatography Heptafluorobutyl chloroformate - based sample preparation protocol for chiral and nonchiral amino acid analysis by gas chromatography
skos:prefLabel
Heptafluorobutyl chloroformate - based sample preparation protocol for chiral and nonchiral amino acid analysis by gas chromatography Heptafluorobutyl chloroformate - based sample preparation protocol for chiral and nonchiral amino acid analysis by gas chromatography
skos:notation
RIV/60077344:_____/12:00369909!RIV12-MSM-60077344
n12:predkladatel
n13:ico%3A60077344
n3:aktivita
n19:Z n19:P
n3:aktivity
P(7F09028), P(GA203/09/2014), P(NS9755), Z(AV0Z50070508)
n3:dodaniDat
n14:2012
n3:domaciTvurceVysledku
n15:8742952 n15:9968709 n15:8519064
n3:druhVysledku
n4:C
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n7:predkladatel
n3:idSjednocenehoVysledku
138824
n3:idVysledku
RIV/60077344:_____/12:00369909
n3:jazykVysledku
n5:eng
n3:klicovaSlova
amino acid; serum; plasma
n3:klicoveSlovo
n18:serum n18:plasma n18:amino%20acid
n3:kontrolniKodProRIV
[FD248E344343]
n3:mistoVydani
New York
n3:nazevZdroje
Amino Acid Analysis. Methods and Protocols
n3:obor
n11:CB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetStranKnihy
363
n3:pocetTvurcuVysledku
3
n3:projekt
n8:GA203%2F09%2F2014 n8:7F09028 n8:NS9755
n3:rokUplatneniVysledku
n14:2012
n3:tvurceVysledku
Hušek, Petr Šimek, Petr Zahradníčková, Helena
n3:zamer
n22:AV0Z50070508
s:numberOfPages
16
n17:doi
10.1007/978-1-61779-445-2_13
n9:hasPublisher
Springer Science & Business Media
n20:isbn
978-1-61779-444-5