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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F11%3A00372946%21RIV12-AV0-60077344
rdf:type
n10:Vysledek skos:Concept
dcterms:description
Bacterial canker of tomato, caused by bacterium Clavibacter michiganensis subsp. michiganensis (Cmm) is a well-known disease resulting in serious losses to both greenhouse and field tomato crops in areas where tomatoes are planted. Cmm was detected and isolated from various parts of strongly infected plants of 10 indeterminate tomato cultivars. The most sensitive technique was optimized PCR with Dreier´s and laboratory-generated PCR primers from the Cmm tomatinase gene. All samples taken from base, stem and petiole of tomato plants were positive in PCR with both primer pairs, and also using IIF and PTA-ELISA in contrast to the samples taken from leaves. Using IIF and PTA-ELISA with PAbs of Neogen Europe Ltd. (UK), Cmm was reliably detected to dilution 1:1000 and 1:100 respectively. Seeds of 4 indeterminate and 4 determinate cultivars were consecutively artificially infected by the mixture of the two reference Cmm strains (BCCM/LMG 7333 and 5727) soaking 48 hours in inoculum (108 CFU/ml). Bacterial canker of tomato, caused by bacterium Clavibacter michiganensis subsp. michiganensis (Cmm) is a well-known disease resulting in serious losses to both greenhouse and field tomato crops in areas where tomatoes are planted. Cmm was detected and isolated from various parts of strongly infected plants of 10 indeterminate tomato cultivars. The most sensitive technique was optimized PCR with Dreier´s and laboratory-generated PCR primers from the Cmm tomatinase gene. All samples taken from base, stem and petiole of tomato plants were positive in PCR with both primer pairs, and also using IIF and PTA-ELISA in contrast to the samples taken from leaves. Using IIF and PTA-ELISA with PAbs of Neogen Europe Ltd. (UK), Cmm was reliably detected to dilution 1:1000 and 1:100 respectively. Seeds of 4 indeterminate and 4 determinate cultivars were consecutively artificially infected by the mixture of the two reference Cmm strains (BCCM/LMG 7333 and 5727) soaking 48 hours in inoculum (108 CFU/ml).
dcterms:title
Detection of Clavibacter michiganensis subsp. michiganensis from tomato plants and seeds using ELISA, IF and PCR with commercial and own primers Detection of Clavibacter michiganensis subsp. michiganensis from tomato plants and seeds using ELISA, IF and PCR with commercial and own primers
skos:prefLabel
Detection of Clavibacter michiganensis subsp. michiganensis from tomato plants and seeds using ELISA, IF and PCR with commercial and own primers Detection of Clavibacter michiganensis subsp. michiganensis from tomato plants and seeds using ELISA, IF and PCR with commercial and own primers
skos:notation
RIV/60077344:_____/11:00372946!RIV12-AV0-60077344
n10:predkladatel
n14:ico%3A60077344
n3:aktivita
n16:Z n16:P
n3:aktivity
P(QH71229), Z(AV0Z50510513)
n3:dodaniDat
n5:2012
n3:domaciTvurceVysledku
n4:8594694 n4:5529182
n3:druhVysledku
n22:D
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
193697
n3:idVysledku
RIV/60077344:_____/11:00372946
n3:jazykVysledku
n6:eng
n3:klicovaSlova
bacterial canker of tomato; ELISA; PCR; IF; detection
n3:klicoveSlovo
n9:detection n9:PCR n9:bacterial%20canker%20of%20tomato n9:ELISA n9:IF
n3:kontrolniKodProRIV
[9640CCC5D8ED]
n3:mistoKonaniAkce
Ischia, Naples
n3:mistoVydani
Leuven
n3:nazevZdroje
III International Symposium on Tomato Diseases
n3:obor
n13:GF
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:projekt
n18:QH71229
n3:rokUplatneniVysledku
n5:2011
n3:tvurceVysledku
Mráz, Ivan Beran, Pavel Kokošková, B.
n3:typAkce
n21:WRD
n3:zahajeniAkce
2010-07-25+02:00
n3:zamer
n8:AV0Z50510513
s:numberOfPages
4
n20:hasPublisher
ISHS Acta Horticulturae
n11:isbn
9789066056145