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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F11%3A00370255%21RIV12-AV0-60077344
rdf:type
n3:Vysledek skos:Concept
dcterms:description
In conifers and other plants with long reproductive cycles, transformed embryogenic calli can serve as a convenient source of plant material for the testing insecticidal or fungicidal transgene efficiency. In this report, transgenic embryogenic callus was obtained after transformation of somatic embryos of Norway spruce (Picea abies) by Agrobacterium tumefaciens with the gus-intron chimeric gene. The stable integration of transgenes was confirmed by PCR and Southern hybridization. The transformation was successful only with suitable embryogenic line sensitive to Agrobacterium. From nine embryogenic lines tested only one gave transgenic callus In conifers and other plants with long reproductive cycles, transformed embryogenic calli can serve as a convenient source of plant material for the testing insecticidal or fungicidal transgene efficiency. In this report, transgenic embryogenic callus was obtained after transformation of somatic embryos of Norway spruce (Picea abies) by Agrobacterium tumefaciens with the gus-intron chimeric gene. The stable integration of transgenes was confirmed by PCR and Southern hybridization. The transformation was successful only with suitable embryogenic line sensitive to Agrobacterium. From nine embryogenic lines tested only one gave transgenic callus
dcterms:title
Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants
skos:prefLabel
Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants
skos:notation
RIV/60077344:_____/11:00370255!RIV12-AV0-60077344
n3:predkladatel
n4:ico%3A60077344
n6:aktivita
n8:Z n8:P
n6:aktivity
P(QH71290), Z(AV0Z50510513)
n6:cisloPeriodika
7
n6:dodaniDat
n15:2012
n6:domaciTvurceVysledku
n17:9784160 n17:2637413 n17:4095154 n17:8199655
n6:druhVysledku
n13:J
n6:duvernostUdaju
n19:S
n6:entitaPredkladatele
n14:predkladatel
n6:idSjednocenehoVysledku
231895
n6:idVysledku
RIV/60077344:_____/11:00370255
n6:jazykVysledku
n16:eng
n6:klicovaSlova
Agrobacterium tumefaciens; genetic engineering; GUS activity; Picea abies (L.) Karst
n6:klicoveSlovo
n12:genetic%20engineering n12:Agrobacterium%20tumefaciens n12:GUS%20activity n12:Picea%20abies%20%28L.%29%20Karst
n6:kodStatuVydavatele
CZ - Česká republika
n6:kontrolniKodProRIV
[F3931224E83E]
n6:nazevZdroje
Journal of Forest Science
n6:obor
n11:EB
n6:pocetDomacichTvurcuVysledku
4
n6:pocetTvurcuVysledku
4
n6:projekt
n18:QH71290
n6:rokUplatneniVysledku
n15:2011
n6:svazekPeriodika
57
n6:tvurceVysledku
Vlasák, Josef Pavingerová, Daniela Bříza, Jindřich Niedermeierová, Hana
n6:zamer
n7:AV0Z50510513
s:issn
1212-4834
s:numberOfPages
4