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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F11%3A00367362%21RIV12-MSM-60077344
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Polymerase chain reaction (PCR) with subsequent restriction fragment length polymorphism (RFLP) analysis is often used for phytoplasma identification and classification. Although these techniques are very sensitive and specific, in some cases, nonspecific reactions, false positives and negatives results as well as unusual or illegible profiles after RFLP analyses, amplification of plant host´s DNA or other difficulties occurred. Experiences with suitability of positive and negative controls integration in PCR, evaluation of critical samples and other difficulties in phytoplasma PCR/RFLP identification are reported. Polymerase chain reaction (PCR) with subsequent restriction fragment length polymorphism (RFLP) analysis is often used for phytoplasma identification and classification. Although these techniques are very sensitive and specific, in some cases, nonspecific reactions, false positives and negatives results as well as unusual or illegible profiles after RFLP analyses, amplification of plant host´s DNA or other difficulties occurred. Experiences with suitability of positive and negative controls integration in PCR, evaluation of critical samples and other difficulties in phytoplasma PCR/RFLP identification are reported.
dcterms:title
Difficulties with conventional phytoplasma diagnostic using PCR/RFLP analyses Difficulties with conventional phytoplasma diagnostic using PCR/RFLP analyses
skos:prefLabel
Difficulties with conventional phytoplasma diagnostic using PCR/RFLP analyses Difficulties with conventional phytoplasma diagnostic using PCR/RFLP analyses
skos:notation
RIV/60077344:_____/11:00367362!RIV12-MSM-60077344
n14:predkladatel
n19:ico%3A60077344
n3:aktivita
n8:P n8:Z
n3:aktivity
P(1QS500510558), P(OC09021), P(QH71145), Z(AV0Z50510513)
n3:cisloPeriodika
Suppl.
n3:dodaniDat
n10:2012
n3:domaciTvurceVysledku
n17:3020630
n3:druhVysledku
n5:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
194600
n3:idVysledku
RIV/60077344:_____/11:00367362
n3:jazykVysledku
n13:eng
n3:klicovaSlova
DNA extraction; PCR; primers; RFLP
n3:klicoveSlovo
n12:RFLP n12:DNA%20extraction n12:primers n12:PCR
n3:kodStatuVydavatele
IT - Italská republika
n3:kontrolniKodProRIV
[A6F36362BB14]
n3:nazevZdroje
Bulletin of Insectology
n3:obor
n9:EE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
1
n3:projekt
n7:1QS500510558 n7:QH71145 n7:OC09021
n3:rokUplatneniVysledku
n10:2011
n3:svazekPeriodika
64
n3:tvurceVysledku
Fránová, Jana
n3:wos
000296681900139
n3:zamer
n16:AV0Z50510513
s:issn
1721-8861
s:numberOfPages
2