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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F11%3A00367360%21RIV12-AV0-60077344
rdf:type
skos:Concept n9:Vysledek
dcterms:description
A Ribes rubrum plant showing malformation and twisting of branches was found in a garden in South Bohemia. Ultrathin sections of tissues from symptomatic shoots revealed the presence of phytoplasma-like bodies. Different primer sets were used for amplification of the 16S-23S ribosomal gene segment. RFLP analysis and sequencing for phytoplasma identification classified the detected phytoplasma in the aster yellows group, subgroup 16SrI-C. Successful transmission of detected phytoplasma by dodder to periwinkle was confirmed by detection of numerous phytoplasma bodies in ultrathin sections of C. roseus and by PCR from dodder and periwinkle. RFLP analyses of PCR products as well as nucleotide sequences of the currant plant and symptomatic periwinkles were identical. Sequenced data obtained from both currant and indicator plant, were aligned and sequences of 1,613 bp were found to be identical. Transmissions of phytoplasma by grafting to healthy currant rootstocks were unsuccessful. A Ribes rubrum plant showing malformation and twisting of branches was found in a garden in South Bohemia. Ultrathin sections of tissues from symptomatic shoots revealed the presence of phytoplasma-like bodies. Different primer sets were used for amplification of the 16S-23S ribosomal gene segment. RFLP analysis and sequencing for phytoplasma identification classified the detected phytoplasma in the aster yellows group, subgroup 16SrI-C. Successful transmission of detected phytoplasma by dodder to periwinkle was confirmed by detection of numerous phytoplasma bodies in ultrathin sections of C. roseus and by PCR from dodder and periwinkle. RFLP analyses of PCR products as well as nucleotide sequences of the currant plant and symptomatic periwinkles were identical. Sequenced data obtained from both currant and indicator plant, were aligned and sequences of 1,613 bp were found to be identical. Transmissions of phytoplasma by grafting to healthy currant rootstocks were unsuccessful.
dcterms:title
Association of aster yellows subgroup 16SrI-C phytoplasmas with a disease of Ribes rubrum Association of aster yellows subgroup 16SrI-C phytoplasmas with a disease of Ribes rubrum
skos:prefLabel
Association of aster yellows subgroup 16SrI-C phytoplasmas with a disease of Ribes rubrum Association of aster yellows subgroup 16SrI-C phytoplasmas with a disease of Ribes rubrum
skos:notation
RIV/60077344:_____/11:00367360!RIV12-AV0-60077344
n9:predkladatel
n17:ico%3A60077344
n6:aktivita
n10:P n10:Z
n6:aktivity
P(QH91224), Z(AV0Z50510513)
n6:cisloPeriodika
Suppl.
n6:dodaniDat
n13:2012
n6:domaciTvurceVysledku
n11:9037098 n11:2845768 n11:6051448
n6:druhVysledku
n12:J
n6:duvernostUdaju
n14:S
n6:entitaPredkladatele
n16:predkladatel
n6:idSjednocenehoVysledku
187325
n6:idVysledku
RIV/60077344:_____/11:00367360
n6:jazykVysledku
n18:eng
n6:klicovaSlova
PCR; RFLP analysis; red currant
n6:klicoveSlovo
n8:PCR n8:red%20currant n8:RFLP%20analysis
n6:kodStatuVydavatele
IT - Italská republika
n6:kontrolniKodProRIV
[1BD132E0371D]
n6:nazevZdroje
Bulletin of Insectology
n6:obor
n15:EE
n6:pocetDomacichTvurcuVysledku
3
n6:pocetTvurcuVysledku
3
n6:projekt
n7:QH91224
n6:rokUplatneniVysledku
n13:2011
n6:svazekPeriodika
64
n6:tvurceVysledku
Špak, Josef Přibylová, Jaroslava Petrzik, Karel
n6:wos
000296681900030
n6:zamer
n19:AV0Z50510513
s:issn
1721-8861
s:numberOfPages
2