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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F11%3A00358982%21RIV12-MSM-60077344
rdf:type
n12:Vysledek skos:Concept
dcterms:description
The subspecies Polynucleobacter necessarius asymbioticus (> 99% 16S rRNA similarity) has a cosmopolitan distribution and a ubiquitous occurrence in freshwater habitats. We tested if the observed ubiquity of these free-living planktonic freshwater bacteria results from a euryoecious (generalist) adaptation of P. n. asymbioticus strains, or from ecological diversification within the subspecies. We developed a reverse line blot hybridization assay enabling the cultivation-independent detection of 13 groups within the subspecies in environmental samples. A set of 121 lentic freshwater habitats, spanning a broad variety of habitat types (e.g. pH levels ranging from 3.8 to 8.5) was investigated for the presence of these 13 P. n. asymbioticus groups. Statistical analyses of the RLBH detections revealed pronounced differences in habitat preferences of several of the groups. Their preferences differed regarding pH, conductivity, dissolved organic carbon and oxygen concentration of habitats. The subspecies Polynucleobacter necessarius asymbioticus (> 99% 16S rRNA similarity) has a cosmopolitan distribution and a ubiquitous occurrence in freshwater habitats. We tested if the observed ubiquity of these free-living planktonic freshwater bacteria results from a euryoecious (generalist) adaptation of P. n. asymbioticus strains, or from ecological diversification within the subspecies. We developed a reverse line blot hybridization assay enabling the cultivation-independent detection of 13 groups within the subspecies in environmental samples. A set of 121 lentic freshwater habitats, spanning a broad variety of habitat types (e.g. pH levels ranging from 3.8 to 8.5) was investigated for the presence of these 13 P. n. asymbioticus groups. Statistical analyses of the RLBH detections revealed pronounced differences in habitat preferences of several of the groups. Their preferences differed regarding pH, conductivity, dissolved organic carbon and oxygen concentration of habitats.
dcterms:title
Ubiquity of Polynucleobacter necessarius subspecies asymbioticus results from ecological diversification Ubiquity of Polynucleobacter necessarius subspecies asymbioticus results from ecological diversification
skos:prefLabel
Ubiquity of Polynucleobacter necessarius subspecies asymbioticus results from ecological diversification Ubiquity of Polynucleobacter necessarius subspecies asymbioticus results from ecological diversification
skos:notation
RIV/60077344:_____/11:00358982!RIV12-MSM-60077344
n12:predkladatel
n13:ico%3A60077344
n4:aktivita
n14:P n14:Z
n4:aktivity
P(GAP504/10/0566), P(MEB060901), Z(AV0Z60170517)
n4:cisloPeriodika
4
n4:dodaniDat
n9:2012
n4:domaciTvurceVysledku
n5:4562275 n5:7194536
n4:druhVysledku
n15:J
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n20:predkladatel
n4:idSjednocenehoVysledku
236441
n4:idVysledku
RIV/60077344:_____/11:00358982
n4:jazykVysledku
n11:eng
n4:klicovaSlova
ubiquity; Polynucleobacter; ecological diversification
n4:klicoveSlovo
n7:ubiquity n7:ecological%20diversification n7:Polynucleobacter
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[729157C7C63D]
n4:nazevZdroje
Environmental Microbiology
n4:obor
n18:EE
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
4
n4:projekt
n8:GAP504%2F10%2F0566 n8:MEB060901
n4:rokUplatneniVysledku
n9:2011
n4:svazekPeriodika
13
n4:tvurceVysledku
Hahn, M. W. Jezberová, Jitka Brandt, U. Jezbera, Jan
n4:wos
000289116700008
n4:zamer
n6:AV0Z60170517
s:issn
1462-2912
s:numberOfPages
10
n19:doi
10.1111/j.1462-2920.2010.02396.x