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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F10%3A00353453%21RIV11-MSM-60077344
rdf:type
skos:Concept n16:Vysledek
dcterms:description
The specific identification of broad tapeworms (genus Diphyllobothrium) infecting humans is very difficult to perform by morphological observation. Molecular analysis by PCR and sequencing represents the only reliable tool to date to identify these parasites to the species level. Due to the recent spread of human diphyllobothriosis in several countries, a correct diagnosis has become crucial to better understand the distribution and the life cycle of human-infecting species as well as to prevent the introduction of parasites to disease-free water systems. Nevertheless, PCR and sequencing are too complicated, long, and expensive for routine diagnostics. In the present study we optimized a cheap and rapid molecular test for the differential identification of the most common Diphyllobothrium species infecting humans (D. latum, D. dendriticum, D. nihonkaiense, and D. pacificum), based on a multiplex PCR with the cytochrome c oxidase subunit 1 gene of mitochondrial DNA. The specific identification of broad tapeworms (genus Diphyllobothrium) infecting humans is very difficult to perform by morphological observation. Molecular analysis by PCR and sequencing represents the only reliable tool to date to identify these parasites to the species level. Due to the recent spread of human diphyllobothriosis in several countries, a correct diagnosis has become crucial to better understand the distribution and the life cycle of human-infecting species as well as to prevent the introduction of parasites to disease-free water systems. Nevertheless, PCR and sequencing are too complicated, long, and expensive for routine diagnostics. In the present study we optimized a cheap and rapid molecular test for the differential identification of the most common Diphyllobothrium species infecting humans (D. latum, D. dendriticum, D. nihonkaiense, and D. pacificum), based on a multiplex PCR with the cytochrome c oxidase subunit 1 gene of mitochondrial DNA.
dcterms:title
Multiplex PCR for Differential Identification of Broad Tapeworms (Cestoda: Diphyllobothrium) Infecting Humans Multiplex PCR for Differential Identification of Broad Tapeworms (Cestoda: Diphyllobothrium) Infecting Humans
skos:prefLabel
Multiplex PCR for Differential Identification of Broad Tapeworms (Cestoda: Diphyllobothrium) Infecting Humans Multiplex PCR for Differential Identification of Broad Tapeworms (Cestoda: Diphyllobothrium) Infecting Humans
skos:notation
RIV/60077344:_____/10:00353453!RIV11-MSM-60077344
n3:aktivita
n7:Z n7:P
n3:aktivity
P(LC522), Z(AV0Z60220518)
n3:cisloPeriodika
9
n3:dodaniDat
n12:2011
n3:domaciTvurceVysledku
n10:7130570 n10:5218136
n3:druhVysledku
n14:J
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
273178
n3:idVysledku
RIV/60077344:_____/10:00353453
n3:jazykVysledku
n17:eng
n3:klicovaSlova
MOLECULAR EVIDENCE; PACIFIC SALMON; NIHONKAIENSE; Diphyllobothrium; multiplex PCR; the cytochrome c oxidase subunit 1; mitochondrial DNA
n3:klicoveSlovo
n4:Diphyllobothrium n4:NIHONKAIENSE n4:the%20cytochrome%20c%20oxidase%20subunit%201 n4:PACIFIC%20SALMON n4:multiplex%20PCR n4:mitochondrial%20DNA n4:MOLECULAR%20EVIDENCE
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[9C0CCB0F4FF4]
n3:nazevZdroje
Journal of Clinical Microbiology
n3:obor
n11:GJ
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
6
n3:projekt
n15:LC522
n3:rokUplatneniVysledku
n12:2010
n3:svazekPeriodika
48
n3:tvurceVysledku
Scholz, Tomáš Wicht, B. Yanagida, T. Brabec, Jan Jiménez, J. A. Ito, A.
n3:wos
000281480400010
n3:zamer
n18:AV0Z60220518
s:issn
0095-1137
s:numberOfPages
6