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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F10%3A00349491%21RIV12-MSM-60077344
rdf:type
n14:Vysledek skos:Concept
dcterms:description
RNA interference (RNAi) is a technique to selectively suppress mRNA of individual genes and, consequently, their cognate proteins. In consideration of large-scale screens to identify candidate drug targets, we examined the selectivity and sensitivity (the degree of suppression) of RNAi for 11 genes produced in different tissues of the parasite: the gut, tegument (surface) and otherwise. We used the schistosomulum stage prepared from infective cercariae larvae which are accessible in large numbers and adaptable to automated screening platforms. We found that RNAi suppresses transcripts selectively, however, the sensitivity of suppression varies. Additionally, we defined operational parameters to facilitate large-scale RNAi, including choice of culture medium, transfection strategy to deliver dsRNA, dose- and time-dependency, and dosing limits. Finally, using fluorescent probes, we show that the developing gut allows rapid entrance of dsRNA into the parasite to initiate RNAi. RNA interference (RNAi) is a technique to selectively suppress mRNA of individual genes and, consequently, their cognate proteins. In consideration of large-scale screens to identify candidate drug targets, we examined the selectivity and sensitivity (the degree of suppression) of RNAi for 11 genes produced in different tissues of the parasite: the gut, tegument (surface) and otherwise. We used the schistosomulum stage prepared from infective cercariae larvae which are accessible in large numbers and adaptable to automated screening platforms. We found that RNAi suppresses transcripts selectively, however, the sensitivity of suppression varies. Additionally, we defined operational parameters to facilitate large-scale RNAi, including choice of culture medium, transfection strategy to deliver dsRNA, dose- and time-dependency, and dosing limits. Finally, using fluorescent probes, we show that the developing gut allows rapid entrance of dsRNA into the parasite to initiate RNAi.
dcterms:title
RNA Interference in Schistosoma mansoni Schistosomula: Selectivity, Sensitivity and Operation for Larger-Scale Screening RNA Interference in Schistosoma mansoni Schistosomula: Selectivity, Sensitivity and Operation for Larger-Scale Screening
skos:prefLabel
RNA Interference in Schistosoma mansoni Schistosomula: Selectivity, Sensitivity and Operation for Larger-Scale Screening RNA Interference in Schistosoma mansoni Schistosomula: Selectivity, Sensitivity and Operation for Larger-Scale Screening
skos:notation
RIV/60077344:_____/10:00349491!RIV12-MSM-60077344
n4:aktivita
n13:P n13:Z
n4:aktivity
P(GA203/09/1585), P(KJB600960911), P(LC06009), P(ME10011), Z(AV0Z40550506), Z(AV0Z60220518)
n4:cisloPeriodika
10
n4:dodaniDat
n9:2012
n4:domaciTvurceVysledku
n5:8511721 n5:2092484
n4:druhVysledku
n16:J
n4:duvernostUdaju
n18:S
n4:entitaPredkladatele
n17:predkladatel
n4:idSjednocenehoVysledku
285527
n4:idVysledku
RIV/60077344:_____/10:00349491
n4:jazykVysledku
n6:eng
n4:klicovaSlova
Tropical disease; Schistosoma; schistosomula; RNAi; protease; tegument; drug target; drug discovery
n4:klicoveSlovo
n10:drug%20target n10:tegument n10:RNAi n10:protease n10:schistosomula n10:Schistosoma n10:Tropical%20disease n10:drug%20discovery
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[2B51C93FFAB4]
n4:nazevZdroje
PLoS Neglected Tropical Diseases
n4:obor
n12:EB
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
11
n4:projekt
n8:LC06009 n8:GA203%2F09%2F1585 n8:ME10011 n8:KJB600960911
n4:rokUplatneniVysledku
n9:2010
n4:svazekPeriodika
4
n4:tvurceVysledku
Caffrey, C. R. Sojka, Daniel Hopkins, S. D. Lim, K.-C. Štefanič, S. Ruelas, D. Suzuki, B. Braschi, S. Horn, Martin Dvořák, Jan McKerrow, J. H.
n4:wos
000283559600019
n4:zamer
n11:AV0Z40550506 n11:AV0Z60220518
s:issn
1935-2735
s:numberOfPages
16
n19:doi
10.1371/journal.pntd.0000850