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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F10%3A00342888%21RIV11-GA0-60077344
rdf:type
skos:Concept n18:Vysledek
dcterms:description
We exploit the tractable reverse genetics of Trypanosoma brucei, the causative agent of African sleeping sickness, in order to specifically analyse the function of PHB in this highly divergent eukaryote. Using inducible RNA interference (RNAi) we show that PHB1 is essential in T brucei, where it is confined to the cell's single mitochondrion forming a high molecular weight complex. PHB1 and PHB2 appear to be indispensible for mitochondrial translation. Their ablation leads to a decrease in mitochondrial membrane potential, however no effect on the level of reactive oxygen species was observed. Flagellates lacking either PHB1 or both PHB1 and PHB2 exhibit significant morphological changes of their organelle, most notably its inflation. Even long after the loss of the PHB proteins, mtDNA was unaltered and mitochondrial cristae remained present, albeit displaced to the periphery of the mitochondrion, which is in contrast to other eukaryotes. We exploit the tractable reverse genetics of Trypanosoma brucei, the causative agent of African sleeping sickness, in order to specifically analyse the function of PHB in this highly divergent eukaryote. Using inducible RNA interference (RNAi) we show that PHB1 is essential in T brucei, where it is confined to the cell's single mitochondrion forming a high molecular weight complex. PHB1 and PHB2 appear to be indispensible for mitochondrial translation. Their ablation leads to a decrease in mitochondrial membrane potential, however no effect on the level of reactive oxygen species was observed. Flagellates lacking either PHB1 or both PHB1 and PHB2 exhibit significant morphological changes of their organelle, most notably its inflation. Even long after the loss of the PHB proteins, mtDNA was unaltered and mitochondrial cristae remained present, albeit displaced to the periphery of the mitochondrion, which is in contrast to other eukaryotes.
dcterms:title
Probing for primary functions of prohibitin in Trypanosoma brucei Probing for primary functions of prohibitin in Trypanosoma brucei
skos:prefLabel
Probing for primary functions of prohibitin in Trypanosoma brucei Probing for primary functions of prohibitin in Trypanosoma brucei
skos:notation
RIV/60077344:_____/10:00342888!RIV11-GA0-60077344
n4:aktivita
n5:Z n5:S n5:P n5:I
n4:aktivity
I, P(GA204/09/1667), P(GP204/06/P423), P(IAA500960705), P(LC07032), S, Z(AV0Z60220518), Z(MSM6007665801)
n4:cisloPeriodika
1
n4:dodaniDat
n14:2011
n4:domaciTvurceVysledku
n10:6548857 n10:1550578 n10:8635137 n10:5698065 n10:8617686 n10:1369490
n4:druhVysledku
n15:J
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n16:predkladatel
n4:idSjednocenehoVysledku
282112
n4:idVysledku
RIV/60077344:_____/10:00342888
n4:jazykVysledku
n8:eng
n4:klicovaSlova
prohibitin; mitochondrion; morphology; mitochondrial translation
n4:klicoveSlovo
n9:prohibitin n9:mitochondrion n9:mitochondrial%20translation n9:morphology
n4:kodStatuVydavatele
AU - Australské společenství
n4:kontrolniKodProRIV
[662267DBE845]
n4:nazevZdroje
International Journal for Parasitology
n4:obor
n13:EB
n4:pocetDomacichTvurcuVysledku
6
n4:pocetTvurcuVysledku
7
n4:projekt
n6:IAA500960705 n6:GA204%2F09%2F1667 n6:LC07032 n6:GP204%2F06%2FP423
n4:rokUplatneniVysledku
n14:2010
n4:svazekPeriodika
40
n4:tvurceVysledku
Vávrová, Zuzana Jirků, Milan Týč, Jiří Faktorová, Drahomíra Kriegová, Eva Lukeš, Julius Maslov, D. A.
n4:wos
000274104300008
n4:zamer
n12:AV0Z60220518 n12:MSM6007665801
s:issn
0020-7519
s:numberOfPages
11