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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F09%3A00334902%21RIV10-MSM-60077344
rdf:type
n12:Vysledek skos:Concept
dcterms:description
Views on myxosporean phylogeny and systematics have recently undergone substantial changes resulting from analyses of SSU rDNA. Here, we further investigate the evolutionary trends within myxosporean lineages by using 35 new sequences of the LSU rDNA. We show a good agreement between the two rRNA genes and confirm the main phylogenetic split between the freshwater and marine lineages. The informative superiority of the LSU data is shown by an increase of the resolution, nodal supports and tree indexes in the LSU rDNA and combined analyses. We determine the most suitable part of LSU for the myxosporean phylogeny by comparing informative content in various regions of the LSU sequences. Based on this comparison, we propose the D5–3-end part of the LSU rRNA gene as the most informative region which provides in concatenation with the complete SSU a well resolved and robust tree. To allow for simple amplification of the marker, we design specific primer set for this part of LSU rDNA. Views on myxosporean phylogeny and systematics have recently undergone substantial changes resulting from analyses of SSU rDNA. Here, we further investigate the evolutionary trends within myxosporean lineages by using 35 new sequences of the LSU rDNA. We show a good agreement between the two rRNA genes and confirm the main phylogenetic split between the freshwater and marine lineages. The informative superiority of the LSU data is shown by an increase of the resolution, nodal supports and tree indexes in the LSU rDNA and combined analyses. We determine the most suitable part of LSU for the myxosporean phylogeny by comparing informative content in various regions of the LSU sequences. Based on this comparison, we propose the D5–3-end part of the LSU rRNA gene as the most informative region which provides in concatenation with the complete SSU a well resolved and robust tree. To allow for simple amplification of the marker, we design specific primer set for this part of LSU rDNA.
dcterms:title
Concatenated SSU and LSU rDNA data confirm the main evolutionary trends within myxosporeans (Myxozoa: Myxosporea) and provide effective tool for their molecular phylogenetics Concatenated SSU and LSU rDNA data confirm the main evolutionary trends within myxosporeans (Myxozoa: Myxosporea) and provide effective tool for their molecular phylogenetics
skos:prefLabel
Concatenated SSU and LSU rDNA data confirm the main evolutionary trends within myxosporeans (Myxozoa: Myxosporea) and provide effective tool for their molecular phylogenetics Concatenated SSU and LSU rDNA data confirm the main evolutionary trends within myxosporeans (Myxozoa: Myxosporea) and provide effective tool for their molecular phylogenetics
skos:notation
RIV/60077344:_____/09:00334902!RIV10-MSM-60077344
n3:aktivita
n14:Z n14:P
n3:aktivity
P(KJB600960701), P(LC522), Z(AV0Z60220518), Z(MSM6007665801)
n3:cisloPeriodika
1
n3:dodaniDat
n13:2010
n3:domaciTvurceVysledku
n4:9394990 n4:9040919 n4:3729346
n3:druhVysledku
n15:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
307998
n3:idVysledku
RIV/60077344:_____/09:00334902
n3:jazykVysledku
n18:eng
n3:klicovaSlova
myxosporea; phylogeny; LBA; LSU rDNA; 28S; SSU rDNA; 18S; D domains
n3:klicoveSlovo
n7:phylogeny n7:18S n7:D%20domains n7:LBA n7:SSU%20rDNA n7:28S n7:myxosporea n7:LSU%20rDNA
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[CFC94AF2726B]
n3:nazevZdroje
Molecular Phylogenetics and Evolution
n3:obor
n16:EG
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n11:LC522 n11:KJB600960701
n3:rokUplatneniVysledku
n13:2009
n3:svazekPeriodika
53
n3:tvurceVysledku
Fiala, Ivan Hypša, Václav Bartošová, Pavla
n3:wos
000269067900008
n3:zamer
n5:AV0Z60220518 n5:MSM6007665801
s:issn
1055-7903
s:numberOfPages
12