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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F09%3A00326242%21RIV10-AV0-60077344
rdf:type
n5:Vysledek skos:Concept
dcterms:description
ELF97 phosphate (ELF-P) is a useful compound for assessing the phosphorus-related status of planktonic aquatic populations. We have used a recently developed protocol that enables the detection by flow cytometry of ELF alcohol (ELFA), the product of ELF-P hydrolysis. The percentage of ELFA-labeled cells, the relative median ELFA fluorescence per cell, and the absolute ELFA fluorescence were determined in both freshwater (lake) and marine samples. ELF97 phosphate (ELF-P) is a useful compound for assessing the phosphorus-related status of planktonic aquatic populations. We have used a recently developed protocol that enables the detection by flow cytometry of ELF alcohol (ELFA), the product of ELF-P hydrolysis. The percentage of ELFA-labeled cells, the relative median ELFA fluorescence per cell, and the absolute ELFA fluorescence were determined in both freshwater (lake) and marine samples.
dcterms:title
Detection of extracellular phosphatase activity at the single-cell level by Enzyme-Labeled Fluorescence and flow cytometry: The importance of time kinetics in ELFA labeling Detection of extracellular phosphatase activity at the single-cell level by Enzyme-Labeled Fluorescence and flow cytometry: The importance of time kinetics in ELFA labeling
skos:prefLabel
Detection of extracellular phosphatase activity at the single-cell level by Enzyme-Labeled Fluorescence and flow cytometry: The importance of time kinetics in ELFA labeling Detection of extracellular phosphatase activity at the single-cell level by Enzyme-Labeled Fluorescence and flow cytometry: The importance of time kinetics in ELFA labeling
skos:notation
RIV/60077344:_____/09:00326242!RIV10-AV0-60077344
n3:aktivita
n10:Z
n3:aktivity
Z(AV0Z60170517)
n3:cisloPeriodika
2
n3:dodaniDat
n17:2010
n3:domaciTvurceVysledku
n6:7264798
n3:druhVysledku
n13:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
309779
n3:idVysledku
RIV/60077344:_____/09:00326242
n3:jazykVysledku
n12:eng
n3:klicovaSlova
alkaline phosphatase; ELF phosphate; heterotrophic bacteria
n3:klicoveSlovo
n14:alkaline%20phosphatase n14:heterotrophic%20bacteria n14:ELF%20phosphate
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[F386593AFCB2]
n3:nazevZdroje
Cytometry. Part A
n3:obor
n16:DA
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
4
n3:rokUplatneniVysledku
n17:2009
n3:svazekPeriodika
75A
n3:tvurceVysledku
Nedoma, Jiří Van Wambeke, F. Duhamel, S. Gregori, G.
n3:wos
000262921900011
n3:zamer
n15:AV0Z60170517
s:issn
1552-4922
s:numberOfPages
6