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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F08%3A00339342%21RIV10-AV0-60077344
rdf:type
n8:Vysledek skos:Concept
dcterms:description
The presence of PPV in sweet cherry (Prunus avium) has been previously reported within an experimental collection of cherry genotypes in Holovousy, Czech Republic. In this paper, the modified procedure of RNA isolation was successfully applied - one cycle of differential ultracentrifugation was inserted before standard RNA isolation using TRI Reagent. Five PPV virus isolates were detected by RT-PCR by commonly used P1/P2 primers. The strain specific PCR and sequence analysis of NIb-CP-3'NCR region (1375 bp length) of PPV isolate 13/2 CZ identified that isolate as member of PPV-Rec group. Studies are in progress to confirm biological transmission of the isolate. The presence of PPV in sweet cherry (Prunus avium) has been previously reported within an experimental collection of cherry genotypes in Holovousy, Czech Republic. In this paper, the modified procedure of RNA isolation was successfully applied - one cycle of differential ultracentrifugation was inserted before standard RNA isolation using TRI Reagent. Five PPV virus isolates were detected by RT-PCR by commonly used P1/P2 primers. The strain specific PCR and sequence analysis of NIb-CP-3'NCR region (1375 bp length) of PPV isolate 13/2 CZ identified that isolate as member of PPV-Rec group. Studies are in progress to confirm biological transmission of the isolate.
dcterms:title
The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic
skos:prefLabel
The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic
skos:notation
RIV/60077344:_____/08:00339342!RIV10-AV0-60077344
n3:aktivita
n16:N n16:Z
n3:aktivity
N, Z(AV0Z50510513)
n3:dodaniDat
n5:2010
n3:domaciTvurceVysledku
n20:3020630
n3:druhVysledku
n15:D
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
385959
n3:idVysledku
RIV/60077344:_____/08:00339342
n3:jazykVysledku
n13:eng
n3:klicovaSlova
Plum pox virus; Prunus avium; sweet cherry
n3:klicoveSlovo
n12:Plum%20pox%20virus n12:Prunus%20avium n12:sweet%20cherry
n3:kontrolniKodProRIV
[55BA99AB15FF]
n3:mistoKonaniAkce
Antalya
n3:mistoVydani
Leueven 1
n3:nazevZdroje
Proceedings of the twentieth international symposium on virus and virus-liked diseases of temperature fruit crops-fruit tree diseases
n3:obor
n7:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n5:2008
n3:tvurceVysledku
Kučerová, J. Navrátil, M. Fránová, Jana Gadiou, S. Šafářová, D. Talacko, L.
n3:typAkce
n19:WRD
n3:wos
000256218900030
n3:zahajeniAkce
2006-05-22+02:00
n3:zamer
n18:AV0Z50510513
s:numberOfPages
6
n11:hasPublisher
ISHS Acta Horticulturea
n10:isbn
978-90-6605-080-8