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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F07%3A00088824%21RIV08-AV0-60077344
rdf:type
n11:Vysledek skos:Concept
dcterms:description
Hydrolytic enzymes involved in rotifer digestive processes were investigated directly at the sites of enzyme action using the ELF (Enzyme Labelled Fluorescence) technique. After enzymatic hydrolysis of an artificial ELF substrate, the fluorescent product ELF alcohol (ELFA) marked the sites of enzyme action. The time development of ELFA labelling was studied at different incubation times. Phosphatases, .beta.-N-acetylhexosaminidases and lipases were examined in Brachionus angularis, B. calyciflorus, Keratella cochlearis and Lecane closterocerca from fed-batch cultures. We detected activities of all studied enzymes mostly in the stomach and intestine of rotifers. L. closterocerca was the only species showing enzyme activity at the mastax. Lipase activity was observed in the stomach and intestine of all species and in the mastax of L. closterocerca. Phosphatases were frequently located at the corona of B. calyciflorus. Trávicí enzymy vířníků byly detekovány a lokalizovány pomocí ELF (Enzyme Labelled Fluorescence) metody. Fluorescenční substrát (ELF97) vytváří precipitáty ELF alkoholu (ELFA) na místě enzymatické aktivity a tím umožňuje její přímou mikroskopickou lokalizaci. Vývoj ELFA značení byl pozorován při různě dlouhých inkubacích. K detekci trávicích enzymů byla nejvhodnější doba inkubace tři hodiny. Fosfatázová, .beta.-N-acetylhexosaminidázová a lipázová aktivita byla studována ve vířnících Brachionus angularis, B. calyciflorus, Keratella cochlearis a Lecane closterocerca z fed-batch kultur. U všech studovaných druhů byla nejčastějším místem aktivity enzymů oblast žaludku a střeva a u L. closterocerca oblast mastaxu. Fosfatázy byly často lokalizovány na vířivém aparátu B. calyciflorus. Hydrolytic enzymes involved in rotifer digestive processes were investigated directly at the sites of enzyme action using the ELF (Enzyme Labelled Fluorescence) technique. After enzymatic hydrolysis of an artificial ELF substrate, the fluorescent product ELF alcohol (ELFA) marked the sites of enzyme action. The time development of ELFA labelling was studied at different incubation times. Phosphatases, .beta.-N-acetylhexosaminidases and lipases were examined in Brachionus angularis, B. calyciflorus, Keratella cochlearis and Lecane closterocerca from fed-batch cultures. We detected activities of all studied enzymes mostly in the stomach and intestine of rotifers. L. closterocerca was the only species showing enzyme activity at the mastax. Lipase activity was observed in the stomach and intestine of all species and in the mastax of L. closterocerca. Phosphatases were frequently located at the corona of B. calyciflorus.
dcterms:title
Rotifer digestive enzymes: direct detection using the ELF technique Rotifer digestive enzymes: direct detection using the ELF technique Trávicí enzymy vířníků: přímá detekce pomocí ELF metody
skos:prefLabel
Rotifer digestive enzymes: direct detection using the ELF technique Rotifer digestive enzymes: direct detection using the ELF technique Trávicí enzymy vířníků: přímá detekce pomocí ELF metody
skos:notation
RIV/60077344:_____/07:00088824!RIV08-AV0-60077344
n4:strany
159;165
n4:aktivita
n12:S n12:P n12:Z
n4:aktivity
P(1QS600170504), P(IAA600170602), P(IAA6017202), S, Z(AV0Z60170517), Z(MSM6007665801)
n4:cisloPeriodika
1
n4:dodaniDat
n13:2008
n4:domaciTvurceVysledku
n15:9040358
n4:druhVysledku
n5:J
n4:duvernostUdaju
n18:S
n4:entitaPredkladatele
n16:predkladatel
n4:idSjednocenehoVysledku
448346
n4:idVysledku
RIV/60077344:_____/07:00088824
n4:jazykVysledku
n14:eng
n4:klicovaSlova
rotifers; enzyme localization; phosphatase; lipase; .beta.-N-acetylhexosaminidase
n4:klicoveSlovo
n6:rotifers n6:lipase n6:enzyme%20localization n6:phosphatase n6:.beta.-N-acetylhexosaminidase
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[7F07EFEF14A7]
n4:nazevZdroje
Hydrobiologia
n4:obor
n17:DA
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
2
n4:projekt
n10:IAA600170602 n10:IAA6017202 n10:1QS600170504
n4:rokUplatneniVysledku
n13:2007
n4:svazekPeriodika
593
n4:tvurceVysledku
Vrba, Jaroslav Štrojsová, M.
n4:zamer
n9:MSM6007665801 n9:AV0Z60170517
s:issn
0018-8158
s:numberOfPages
7