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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F07%3A00086809%21RIV08-AV0-60077344
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Studovali jsme afinitu extracelulárních fosfatáz pro substrát ELF97 fosfát (ELFP) v různých akvatických ekosystémech nepřímou metodou založenou na inhibici hydrolýzy 4-metylumbeliferyl fosfátu. Hdnoty inhibiční konstanty, Ki, které odpovídají koncentraci potřebné pro 50-% saturaci enzymu ELFP, byly nejnižší v oligotrofní oblasti Středozemního moře, vyšší v acidifikovaných jezerech, eutrofní přehradě a v kultuře bakterie Alteromonas infernus. Naše pokusy ukazují že koncetrace ELFP v kitu na měření endogenních fosfatáz (Molecular Probes) zajišťuje >99% saturaci extracelulárních fosfatáz v moři a >90% saturaci ve sladkých vodách. We indirectly studied the affinity of extracellular phosphatases for ELF97 phosphate (ELFP) in different aquatic environments through its ability to inhibit the hydrolysis of 4-methylumbelliferyl phosphate. Values of inhibition constants, Ki, which correspond to the concentrations necessary for half saturation of phosphatases by ELFP,were lowest in the oligotrophic Mediterranean Sea.We found higher values in oligo- to mesotrophic acidified lakes, in a eutrophic reservoir and in a pure culture of the marine bacterium Alteromonas infernus. Our experiments indicated that the assay ELFP concentration in the endogeneous phosphatase detection kit (Molecular Probes) used would ensure >99% saturation of extracellular phosphatases in marine environments and >90% saturation in the studied fresh waters. We indirectly studied the affinity of extracellular phosphatases for ELF97 phosphate (ELFP) in different aquatic environments through its ability to inhibit the hydrolysis of 4-methylumbelliferyl phosphate. Values of inhibition constants, Ki, which correspond to the concentrations necessary for half saturation of phosphatases by ELFP,were lowest in the oligotrophic Mediterranean Sea.We found higher values in oligo- to mesotrophic acidified lakes, in a eutrophic reservoir and in a pure culture of the marine bacterium Alteromonas infernus. Our experiments indicated that the assay ELFP concentration in the endogeneous phosphatase detection kit (Molecular Probes) used would ensure >99% saturation of extracellular phosphatases in marine environments and >90% saturation in the studied fresh waters.
dcterms:title
Affinity of extracellular phosphatases for ELF97 phosphate in aquatic environments Afinita extracelulárních fosfatáz pro ELF97 fosfát ve vodním prostředí Affinity of extracellular phosphatases for ELF97 phosphate in aquatic environments
skos:prefLabel
Affinity of extracellular phosphatases for ELF97 phosphate in aquatic environments Affinity of extracellular phosphatases for ELF97 phosphate in aquatic environments Afinita extracelulárních fosfatáz pro ELF97 fosfát ve vodním prostředí
skos:notation
RIV/60077344:_____/07:00086809!RIV08-AV0-60077344
n3:strany
454;460
n3:aktivita
n5:Z n5:P n5:V
n3:aktivity
P(IAA600170602), P(IAA6017202), V, Z(AV0Z60170517)
n3:cisloPeriodika
5
n3:dodaniDat
n9:2008
n3:domaciTvurceVysledku
n10:9408150 n10:7264798 n10:9479465
n3:druhVysledku
n16:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
408715
n3:idVysledku
RIV/60077344:_____/07:00086809
n3:jazykVysledku
n13:eng
n3:klicovaSlova
ELF97 phosphate; microbial phosphatases; affinity
n3:klicoveSlovo
n4:ELF97%20phosphate n4:affinity n4:microbial%20phosphatases
n3:kodStatuVydavatele
AU - Australské společenství
n3:kontrolniKodProRIV
[5D99F62681C2]
n3:nazevZdroje
Marine and Freshwater Research
n3:obor
n14:DA
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
5
n3:projekt
n15:IAA6017202 n15:IAA600170602
n3:rokUplatneniVysledku
n9:2007
n3:svazekPeriodika
58
n3:tvurceVysledku
Štrojsová, Alena Štrojsová, Martina Van Wambeke, F. Duhamel, S. Nedoma, Jiří
n3:zamer
n8:AV0Z60170517
s:issn
1323-1650
s:numberOfPages
7