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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F06%3A00047194%21RIV07-AV0-60077344
rdf:type
n12:Vysledek skos:Concept
dcterms:description
An experiment designed to examine food preferences of heterotrophic nanoflagellates grazing on bacterioplankton was performed in the freshwater Římov reservoir. Water samples were size-fractionated to obtain <5um filtrate containing bacteria and HNF. To manipulate resource availability,<5um treatments were incubated in dialysis bags submerged in the barrels filled with the unfiltered reservoir water amended with either orthophosphate or glucose or combination of both. We employed r-RNA-targeted probes to assess HNF prey preferences by analyzing bacterial prey in HNF food vacuoles compared to available bacteria. Actinobacteria were avoided by HNF in all treatments. Cytophaga-Flavobacterium-Bacteroidetes bacteria were positively selected mainly in treatments in which bacteria were heavily grazed,the <5um treatments, but this trend was less pronounced towards the end of the study. The members of a small subcluster of Betaproteobacteria were mostly positively selected. Experiment sledující potravní preference prvoků byl proveden na sladkovodní nádrži Římov. Vzorky vody byly velikostně frakcionovány, abychom dostali frakci menší než 0,5 mikrometrů obsahující bakterie a heterotrofní prvoky. Abychom mohli manipulovat dostupnost nutrientů, 5 mikrometrové frakce byly inkubovány v dialyzačních sáčcích ponořených v barelech naplněných nefiltrovanou vodou z nádrže, obohacenou o fosfát, glukózu, případně obojí. Byl aplikovány genetické próby přímo k určení potravních preferencí prvoků analýzou jejich potravních vakuol. Bakterie patřící mezi Actinobacteria byly negativně selektovány ve všech variantách. Bakterie patřící do skupiny Cytophaga-Flavobacterium-Bacteroidetes byly vesměs pozitivně selektovány, hlavně ve variantách se silným predačním tlakem vyvíjeným na bakterie. Tento trend se měnil s postupujícím pokusem. Podskupina bakterií patřících do skupiny Betaproteobacteria byla prvoky pozitivně přijímána. An experiment designed to examine food preferences of heterotrophic nanoflagellates grazing on bacterioplankton was performed in the freshwater Římov reservoir. Water samples were size-fractionated to obtain <5um filtrate containing bacteria and HNF. To manipulate resource availability,<5um treatments were incubated in dialysis bags submerged in the barrels filled with the unfiltered reservoir water amended with either orthophosphate or glucose or combination of both. We employed r-RNA-targeted probes to assess HNF prey preferences by analyzing bacterial prey in HNF food vacuoles compared to available bacteria. Actinobacteria were avoided by HNF in all treatments. Cytophaga-Flavobacterium-Bacteroidetes bacteria were positively selected mainly in treatments in which bacteria were heavily grazed,the <5um treatments, but this trend was less pronounced towards the end of the study. The members of a small subcluster of Betaproteobacteria were mostly positively selected.
dcterms:title
Prey selectivity of bacterivorous protists in different size fractions of reservoir water amended with nutrients Potravní preference prvoků v rozdílných frakcích vody obohacené o nutrienty Prey selectivity of bacterivorous protists in different size fractions of reservoir water amended with nutrients
skos:prefLabel
Potravní preference prvoků v rozdílných frakcích vody obohacené o nutrienty Prey selectivity of bacterivorous protists in different size fractions of reservoir water amended with nutrients Prey selectivity of bacterivorous protists in different size fractions of reservoir water amended with nutrients
skos:notation
RIV/60077344:_____/06:00047194!RIV07-AV0-60077344
n3:strany
1330;1339
n3:aktivita
n11:P n11:Z
n3:aktivity
P(GA206/05/0007), Z(AV0Z60170517), Z(MSM6007665801)
n3:cisloPeriodika
8
n3:dodaniDat
n10:2007
n3:domaciTvurceVysledku
n15:6252591 n15:7194536 n15:3267253
n3:druhVysledku
n18:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
494412
n3:idVysledku
RIV/60077344:_____/06:00047194
n3:jazykVysledku
n16:eng
n3:klicovaSlova
selectivity; flagellates; grazing; fluorescence; reservoir; nutrients
n3:klicoveSlovo
n6:grazing n6:nutrients n6:flagellates n6:selectivity n6:reservoir n6:fluorescence
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[93C5E2D6A77E]
n3:nazevZdroje
Environmental Microbiology
n3:obor
n13:EE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n14:GA206%2F05%2F0007
n3:rokUplatneniVysledku
n10:2006
n3:svazekPeriodika
8
n3:tvurceVysledku
Horňák, Karel Šimek, Karel Jezbera, Jan
n3:zamer
n4:AV0Z60170517 n4:MSM6007665801
s:issn
1462-2912
s:numberOfPages
10