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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F06%3A00047087%21RIV07-AV0-60077344
rdf:type
n3:Vysledek skos:Concept
dcterms:description
Metabolism of the solvents N,N-dimethylformamide (DMF) and N-methylformamide (MF) results in the formation of N-methylcarbamoyl adducts at the N-terminal valine and lysine in blood protein globin. Globin from exposed subjects to DMF (n = 35) and unexposed controls (n = 5) was analyzed by 2 methods. Edman degradation was used as to measure the valine adduct by converting it to 3-methyl-5-isopropylhydantoin (MVH). The MVH levels in globin of the exposed subjects were in the range of 1-441 nmol/g, in controls < 1 nmol/g. The principal method involved enzymatic hydrolysis with pronase to release free N-epsilon-(N-methylcarbamoyl)lysine (MLU) and N-methyl-carbamoylvaline (MVU), which were directly determined by HPLC/MS/MS. MLU was found in most globins from the exposed subjects but not in the controls. A close correlation between the MLU and MVH levels (nmol/g) was observed: MLU = 7 + 0.48 MVH (R-2 = 0.84, n = 32). Metabolismus rozpouštědel N,N-dimethylformamidu (DMF) a N-methylformamidu (MF) vede ke vzniku N-methylkarbamoylových aduktů s krevním globinem na N-terminálovém valinu a lysinu. Globin osob vystavených DMF (n = 35 ) a kontroly (n = 5) byly analyzovány dvěma metodami. Edmanova degradace byla použita k měření valinového aduktu 3-methyl-5-isopropylhydantoinu (MVH). MVH hladiny u exponovaných osob byly v rozmezí 1-441 nmol/g, v kontrolách < 1 nmol/g. Hlavní použitá metoda zahrnovala enzymatickou pronázovou hydrolýzu a následnou HPLC/M/MS analýzu N-epsilon-(N-methylkarbamoyl)lysinu (MLU) and N-methyl-karbamoylvaline (MVU). MLU byl nalezen ve většině globinů u exponovaných subjektů, ale ne v kontrolách. Byla pozorována blízká korelace mezi hladinami MLU a MVH (nmol/g) : MLU = 7 + 0.48 MVH (R-2 = 0.84, n = 32). Poznatky naznačují dlouhodobou persitenci MLU v lidském těle, a tak jeho potenciál posloužit jako biomarker chronické expozice DMF. Metabolism of the solvents N,N-dimethylformamide (DMF) and N-methylformamide (MF) results in the formation of N-methylcarbamoyl adducts at the N-terminal valine and lysine in blood protein globin. Globin from exposed subjects to DMF (n = 35) and unexposed controls (n = 5) was analyzed by 2 methods. Edman degradation was used as to measure the valine adduct by converting it to 3-methyl-5-isopropylhydantoin (MVH). The MVH levels in globin of the exposed subjects were in the range of 1-441 nmol/g, in controls < 1 nmol/g. The principal method involved enzymatic hydrolysis with pronase to release free N-epsilon-(N-methylcarbamoyl)lysine (MLU) and N-methyl-carbamoylvaline (MVU), which were directly determined by HPLC/MS/MS. MLU was found in most globins from the exposed subjects but not in the controls. A close correlation between the MLU and MVH levels (nmol/g) was observed: MLU = 7 + 0.48 MVH (R-2 = 0.84, n = 32).
dcterms:title
N-Methylcarbamoyl-lysine adduct in globin: a new metabolic product and potential biomarker of N,N-dimethylformamide in humans N-Methylcarbamoyl-lysine adduct in globin: a new metabolic product and potential biomarker of N,N-dimethylformamide in humans Adukt N-Methylkarbamoyl-lysinu v hemoglobinu: nový metabolický produkt a potenciální biomarker expozice N,N-dimethylformamidu u člověka
skos:prefLabel
N-Methylcarbamoyl-lysine adduct in globin: a new metabolic product and potential biomarker of N,N-dimethylformamide in humans N-Methylcarbamoyl-lysine adduct in globin: a new metabolic product and potential biomarker of N,N-dimethylformamide in humans Adukt N-Methylkarbamoyl-lysinu v hemoglobinu: nový metabolický produkt a potenciální biomarker expozice N,N-dimethylformamidu u člověka
skos:notation
RIV/60077344:_____/06:00047087!RIV07-AV0-60077344
n4:strany
211;218
n4:aktivita
n12:P n12:Z
n4:aktivity
P(GA303/00/0923), Z(AV0Z50070508)
n4:cisloPeriodika
2-3
n4:dodaniDat
n5:2007
n4:domaciTvurceVysledku
n10:3852067 n10:8519064
n4:druhVysledku
n13:J
n4:duvernostUdaju
n15:S
n4:entitaPredkladatele
n14:predkladatel
n4:idSjednocenehoVysledku
488732
n4:idVysledku
RIV/60077344:_____/06:00047087
n4:jazykVysledku
n7:eng
n4:klicovaSlova
N,N-dimethylformamide; globin; adducts
n4:klicoveSlovo
n9:N-dimethylformamide n9:adducts n9:N n9:globin
n4:kodStatuVydavatele
IE - Irsko
n4:kontrolniKodProRIV
[D0AC7AA516A4]
n4:nazevZdroje
Toxicology Letters
n4:obor
n18:CE
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
6
n4:projekt
n16:GA303%2F00%2F0923
n4:rokUplatneniVysledku
n5:2006
n4:svazekPeriodika
162
n4:tvurceVysledku
Šimek, Petr Kičová, R. Nohová, H. Cimlová, Jana Stránský, V. Mráz, J.
n4:zamer
n17:AV0Z50070508
s:issn
0378-4274
s:numberOfPages
8