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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F06%3A00042946%21RIV07-AV0-60077344
rdf:type
n17:Vysledek skos:Concept
dcterms:description
Activity of extracellular acid phosphatases was measured at single-cell level in bacterioplankton groups defined by their morphology and size, in acidified mountain Lake Čertovo, using the substrate ELF97 phosphate. Fluorescent precipitates resulting from the substrate hydrolysis by phosphatases were quantified by image analysis. A conspicuous morphotype of curved cells, despite its low total biomass (average of 4%), contributed significantly (in average by 31%) to the total bacterioplankton phosphatase activity. Bacterial filaments, although comprising in average 85% of bacterioplankton biomass, contributed to the total bacterioplankton activity only by 45%. Biomass-specific activity of extracellular (cell-surface) phosphatases of the main bacterioplankton morphotypes increased in the order: filaments < cocci and rods < curved cells. The biomass-specific activity of bacterioplankton extracellular phosphatases was generally highest in the spring and decreased gradually during summer. Activity of extracellular acid phosphatases was measured at single-cell level in bacterioplankton groups defined by their morphology and size, in acidified mountain Lake Čertovo, using the substrate ELF97 phosphate. Fluorescent precipitates resulting from the substrate hydrolysis by phosphatases were quantified by image analysis. A conspicuous morphotype of curved cells, despite its low total biomass (average of 4%), contributed significantly (in average by 31%) to the total bacterioplankton phosphatase activity. Bacterial filaments, although comprising in average 85% of bacterioplankton biomass, contributed to the total bacterioplankton activity only by 45%. Biomass-specific activity of extracellular (cell-surface) phosphatases of the main bacterioplankton morphotypes increased in the order: filaments < cocci and rods < curved cells. The biomass-specific activity of bacterioplankton extracellular phosphatases was generally highest in the spring and decreased gradually during summer. V acidifikovaném Čertově jezeře byla pomocí fluorogenního substrátu ELF97měřena za použití pomocí analýzy obrazu aktivita kyselých fosfatáz vázaných na povrch bakteriálních buněk. Najvyšší aktivita byla zjištěna u specifického morfotypu zahnutých buněk, jejichž podíl na biomase bakterioplanktonu činil v sezonním průměru 4%, zatímco jejich podíl na aktivitě enzymu byl 31%. Vláknité bakterie tvořily naopak 85% biomasy, ale jejich podíl na aktivitě enzymu byl jen 45%. Specifická aktivita vztažená na biomasu byla nejvyšší na jaře a snižovala se v průběhu sezony, byla nejvyšší u zahnutých buněk, střední u kokálních a tyčinkovitých buněk, a nejnižší u vláken.
dcterms:title
Specific activity of cell-surface acid phosphatase in different bacterioplankton morphotypes in an acidified mountain lake Specifická aktivita fosfatázy vázané na povrch buněk u různých morfotypů bakterioplanktonu v acidifikovaném horském jezeře Specific activity of cell-surface acid phosphatase in different bacterioplankton morphotypes in an acidified mountain lake
skos:prefLabel
Specifická aktivita fosfatázy vázané na povrch buněk u různých morfotypů bakterioplanktonu v acidifikovaném horském jezeře Specific activity of cell-surface acid phosphatase in different bacterioplankton morphotypes in an acidified mountain lake Specific activity of cell-surface acid phosphatase in different bacterioplankton morphotypes in an acidified mountain lake
skos:notation
RIV/60077344:_____/06:00042946!RIV07-AV0-60077344
n4:strany
1271;1279
n4:aktivita
n10:V n10:Z n10:P
n4:aktivity
P(IAA6017202), V, Z(AV0Z60170517)
n4:cisloPeriodika
7
n4:dodaniDat
n12:2007
n4:domaciTvurceVysledku
n15:7264798 n15:9040358
n4:druhVysledku
n16:J
n4:duvernostUdaju
n14:S
n4:entitaPredkladatele
n5:predkladatel
n4:idSjednocenehoVysledku
500687
n4:idVysledku
RIV/60077344:_____/06:00042946
n4:jazykVysledku
n18:eng
n4:klicovaSlova
alkaline phosphatase; bacterial morphorypes; acidified lake
n4:klicoveSlovo
n8:bacterial%20morphorypes n8:acidified%20lake n8:alkaline%20phosphatase
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[12E9D4ADC007]
n4:nazevZdroje
Environmental Microbiology
n4:obor
n7:CE
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
2
n4:projekt
n13:IAA6017202
n4:rokUplatneniVysledku
n12:2006
n4:svazekPeriodika
8
n4:tvurceVysledku
Nedoma, Jiří Vrba, Jaroslav
n4:zamer
n11:AV0Z60170517
s:issn
1462-2912
s:numberOfPages
9