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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F06%3A00032051%21RIV07-AV0-60077344
rdf:type
skos:Concept n15:Vysledek
dcterms:description
For the first time, headspace solid-phase microextraction coupled with GC-MS analysis was used to study volatile compounds emitted by the tick Ixodes ricinus (L.). Variables such as the type of SPME fibre, equilibration time and extraction time have been evaluated with the mixture of four selected standards, a so-called artificial tick (acetophenone, racemic 4-heptanolide, methyl 2-methoxybenzoate and methyl 3-chloro-4-methoxybenzoate). Optimized conditions were obtained by the use of PDMS 100 µm fibre at 30 min equilibration and 15 min extraction time. The method proved to have a good linearity at a concentration range from 0.5 ng (1 ng for methyl 3-chloro-4-methoxybenzoate) to 25 ng. LODs for a compound ranged between 0.19 and 1 ng, RSD (%) ranged from 13.76 to 25.08. The determination of 1.99 ng of methyl 3-chloro-4-methoxybenzoate emitted by five engorged females proved the usefulness of the developed method to identify and quantify the volatile compounds emitted by ticks. Metoda headspace SPME spojená s GC-MS analýzou byla poprvé použita pro studium těkavých látek vylučovaných klíštětem Ixodes ricinus (L.) Pro směs čtyř vybraných standardů, tzv. “umělé klíště” (acetofenon, racemický 4-heptanolid, methyl 2-methoxybenzoát a methyl 3-chloro-4-methoxybenzoát) byly testovány tyto proměnné: typ SPME vlákna, doba ekvilibrace a doba extrakce. Jako optimální podmínky byly nalezeny: vlákno PDMS 100 µm, 30 min ekvilibrace a 15 min extrakce. Metoda vykazovala dobrou linearitu v koncentračním rozmezí od 0,5 ng (1 ng for methyl 3-chloro-4-methoxybenzoát) do 25 ng. Limity detekce látek se pohybovaly v rozmezí 0,19 až 1 ng, RSD (%) od 13,76 do 25,08. Pomocí této vyvinuté metody bylo stanoveno 1,99 ng methyl 3-chloro-4-methoxybenzoátu emitovaného pěti nasátými samicemi. Byla tak dokázána schopnost metody identifikovat a kvantifikovat těkavé látky vylučované klíšťaty. For the first time, headspace solid-phase microextraction coupled with GC-MS analysis was used to study volatile compounds emitted by the tick Ixodes ricinus (L.). Variables such as the type of SPME fibre, equilibration time and extraction time have been evaluated with the mixture of four selected standards, a so-called artificial tick (acetophenone, racemic 4-heptanolide, methyl 2-methoxybenzoate and methyl 3-chloro-4-methoxybenzoate). Optimized conditions were obtained by the use of PDMS 100 µm fibre at 30 min equilibration and 15 min extraction time. The method proved to have a good linearity at a concentration range from 0.5 ng (1 ng for methyl 3-chloro-4-methoxybenzoate) to 25 ng. LODs for a compound ranged between 0.19 and 1 ng, RSD (%) ranged from 13.76 to 25.08. The determination of 1.99 ng of methyl 3-chloro-4-methoxybenzoate emitted by five engorged females proved the usefulness of the developed method to identify and quantify the volatile compounds emitted by ticks.
dcterms:title
Optimalizace metody SPME pro stanovení těkavých látek vylučovaných klíštětem Ixodes ricinus (L.) Optimization of the solid-phase microextraction method in the determination of Ixodes ricinus (L.) volatiles Optimization of the solid-phase microextraction method in the determination of Ixodes ricinus (L.) volatiles
skos:prefLabel
Optimization of the solid-phase microextraction method in the determination of Ixodes ricinus (L.) volatiles Optimization of the solid-phase microextraction method in the determination of Ixodes ricinus (L.) volatiles Optimalizace metody SPME pro stanovení těkavých látek vylučovaných klíštětem Ixodes ricinus (L.)
skos:notation
RIV/60077344:_____/06:00032051!RIV07-AV0-60077344
n3:strany
236;241
n3:aktivita
n11:Z n11:P
n3:aktivity
P(GA206/04/0751), Z(AV0Z50070508), Z(AV0Z60220518)
n3:cisloPeriodika
2
n3:dodaniDat
n9:2007
n3:domaciTvurceVysledku
n10:9968709 n10:5157641
n3:druhVysledku
n17:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
490938
n3:idVysledku
RIV/60077344:_____/06:00032051
n3:jazykVysledku
n6:eng
n3:klicovaSlova
GC/MS; Ixodes ricinus; solid-phase microextraction
n3:klicoveSlovo
n13:Ixodes%20ricinus n13:solid-phase%20microextraction n13:GC%2FMS
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[ABEBEB18679C]
n3:nazevZdroje
Journal of Separation Science
n3:obor
n8:CB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n18:GA206%2F04%2F0751
n3:rokUplatneniVysledku
n9:2006
n3:svazekPeriodika
29
n3:tvurceVysledku
Zahradníčková, Helena Bouman, Edwin Arien Poul
n3:zamer
n16:AV0Z60220518 n16:AV0Z50070508
s:issn
1615-9306
s:numberOfPages
6