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Statements

Subject Item
n2:RIV%2F60076658%3A12640%2F09%3A00010037%21RIV10-MSM-12640___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Aromatic amino acids play an important role in ultraviolet (UV)-induced photochemical reactions in proteins. In this work, we aim at gaining insight into the photochemical reactions induced by near-UV light excitation of aromatic residues that lead to breakage of disulfide bridges in our model enzyme, Fusarium solani pisi cutinase, a lipolytic enzyme. With this purpose, we acquired transient absorption data of cutinase, with supplemental experimental data on tryptophan (Trp) and lysozyme as reference molecules. We here report formation kinetics and lifetimes of transient chemical species created upon UV excitation of aromatic residues in proteins. Two proteins, lysozyme and cutinase, as well as the free amino acid Trp, were studied under acidic, neutral, and alkaline conditions. The shortest-lived species is assigned to solvated electrons (lifetimes of a few microseconds to nanoseconds), whereas the longer-lived species are assigned to aromatic neutral and ionic radicals, Trp triplet states, and radic Aromatic amino acids play an important role in ultraviolet (UV)-induced photochemical reactions in proteins. In this work, we aim at gaining insight into the photochemical reactions induced by near-UV light excitation of aromatic residues that lead to breakage of disulfide bridges in our model enzyme, Fusarium solani pisi cutinase, a lipolytic enzyme. With this purpose, we acquired transient absorption data of cutinase, with supplemental experimental data on tryptophan (Trp) and lysozyme as reference molecules. We here report formation kinetics and lifetimes of transient chemical species created upon UV excitation of aromatic residues in proteins. Two proteins, lysozyme and cutinase, as well as the free amino acid Trp, were studied under acidic, neutral, and alkaline conditions. The shortest-lived species is assigned to solvated electrons (lifetimes of a few microseconds to nanoseconds), whereas the longer-lived species are assigned to aromatic neutral and ionic radicals, Trp triplet states, and radic
dcterms:title
Flash Photolysis of Cutinase: Identification and Decay Kinetics of Transient Intermediates Formed upon UV Excitation of Aromatic Residues Flash Photolysis of Cutinase: Identification and Decay Kinetics of Transient Intermediates Formed upon UV Excitation of Aromatic Residues
skos:prefLabel
Flash Photolysis of Cutinase: Identification and Decay Kinetics of Transient Intermediates Formed upon UV Excitation of Aromatic Residues Flash Photolysis of Cutinase: Identification and Decay Kinetics of Transient Intermediates Formed upon UV Excitation of Aromatic Residues
skos:notation
RIV/60076658:12640/09:00010037!RIV10-MSM-12640___
n4:aktivita
n12:V
n4:aktivity
V
n4:cisloPeriodika
1
n4:dodaniDat
n8:2010
n4:domaciTvurceVysledku
n10:4395263
n4:druhVysledku
n16:J
n4:duvernostUdaju
n11:S
n4:entitaPredkladatele
n14:predkladatel
n4:idSjednocenehoVysledku
315196
n4:idVysledku
RIV/60076658:12640/09:00010037
n4:jazykVysledku
n15:eng
n4:klicovaSlova
EXCITED-STATE CHEMISTRY; ABSORBING AMINO-ACIDS; HYDRATED ELECTRON; AQUEOUS-SOLUTIONS; PULSE-RADIOLYSIS; LIQUID WATER; SIMPLE DERIVATIVES; GLOBAL ANALYSIS; RATE CONSTANTS; TRYPTOPHAN
n4:klicoveSlovo
n5:GLOBAL%20ANALYSIS n5:SIMPLE%20DERIVATIVES n5:AQUEOUS-SOLUTIONS n5:RATE%20CONSTANTS n5:TRYPTOPHAN n5:ABSORBING%20AMINO-ACIDS n5:PULSE-RADIOLYSIS n5:HYDRATED%20ELECTRON n5:EXCITED-STATE%20CHEMISTRY n5:LIQUID%20WATER
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[1CE5E2A353F1]
n4:nazevZdroje
Biophysical Journal
n4:obor
n13:BO
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
8
n4:rokUplatneniVysledku
n8:2009
n4:svazekPeriodika
97
n4:tvurceVysledku
Pascher, Torbjorn Yartsev, Arkady Polívka, Tomáš Skovsen, Esben Sundstrom, Villly Klitgaard, Soren Petersen, Steffen B Neves-Petersen, Maria Teresa
n4:wos
000267871000021
s:issn
0006-3495
s:numberOfPages
16
n9:organizacniJednotka
12640