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Statements

Subject Item
n2:RIV%2F60076658%3A12520%2F14%3A43886915%21RIV15-MSM-12520___
rdf:type
skos:Concept n13:Vysledek
rdfs:seeAlso
http://link.springer.com/article/10.2478%2Fs11756-013-0285-0
dcterms:description
Planktonic crustaceans are traditionally identified based on morphological and morphometric characters. However, such characters may be hardly distinguishable and often overlap between species. A probability of misidentification is thus relatively high. Molecular techniques may increase the accuracy of identification if appropriate markers are used. Aim of our work was to develop a simple molecular procedure enabling discrimination between four species of Simocephalus occurring in Europe. PCR-RFLP technique proved to be suitable for such discrimination. Within the 709 bp fragment of mitochondrial cytochrome c oxidase subunit 1 gene we found unique combinations of restriction sites of the BbsI and SacI enzymes for Simocephalus vetulus, S. exspinosus, S. serrulatus and S. congener. PCR products of samples from several locations in Slovakia were digested with the two enzymes and electrophoresed on an agarose gel. The restriction patterns were clearly visible and easily distinguishable. This method is applicable for identifying the four species in any life-stage. Considering its simplicity and cost-effectiveness it can be widely used as a diagnostic tool for discriminating between Simocephalus species with overlapping morphologic characters. Planktonic crustaceans are traditionally identified based on morphological and morphometric characters. However, such characters may be hardly distinguishable and often overlap between species. A probability of misidentification is thus relatively high. Molecular techniques may increase the accuracy of identification if appropriate markers are used. Aim of our work was to develop a simple molecular procedure enabling discrimination between four species of Simocephalus occurring in Europe. PCR-RFLP technique proved to be suitable for such discrimination. Within the 709 bp fragment of mitochondrial cytochrome c oxidase subunit 1 gene we found unique combinations of restriction sites of the BbsI and SacI enzymes for Simocephalus vetulus, S. exspinosus, S. serrulatus and S. congener. PCR products of samples from several locations in Slovakia were digested with the two enzymes and electrophoresed on an agarose gel. The restriction patterns were clearly visible and easily distinguishable. This method is applicable for identifying the four species in any life-stage. Considering its simplicity and cost-effectiveness it can be widely used as a diagnostic tool for discriminating between Simocephalus species with overlapping morphologic characters.
dcterms:title
Discrimination between four Simocephalus species from Slovakia using a PCR-RFLP technique Discrimination between four Simocephalus species from Slovakia using a PCR-RFLP technique
skos:prefLabel
Discrimination between four Simocephalus species from Slovakia using a PCR-RFLP technique Discrimination between four Simocephalus species from Slovakia using a PCR-RFLP technique
skos:notation
RIV/60076658:12520/14:43886915!RIV15-MSM-12520___
n4:aktivita
n17:I
n4:aktivity
I
n4:cisloPeriodika
1
n4:dodaniDat
n10:2015
n4:domaciTvurceVysledku
n9:7725582
n4:druhVysledku
n14:J
n4:duvernostUdaju
n19:S
n4:entitaPredkladatele
n7:predkladatel
n4:idSjednocenehoVysledku
11702
n4:idVysledku
RIV/60076658:12520/14:43886915
n4:jazykVysledku
n11:eng
n4:klicovaSlova
Cladocera; restriction fragment length polymorphism; species identification; Anomopoda; cytochrome oxidase I
n4:klicoveSlovo
n6:Anomopoda n6:cytochrome%20oxidase%20I n6:restriction%20fragment%20length%20polymorphism n6:species%20identification n6:Cladocera
n4:kodStatuVydavatele
PL - Polská republika
n4:kontrolniKodProRIV
[D81ADF0F161A]
n4:nazevZdroje
Biologia
n4:obor
n12:GL
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
4
n4:rokUplatneniVysledku
n10:2014
n4:svazekPeriodika
69
n4:tvurceVysledku
Ciampor, Fedor Ciamporova-Zatovicova, Zuzana Kohout, Jan Illyova, Marta
n4:wos
000327081400009
s:issn
0006-3088
s:numberOfPages
4
n18:doi
10.2478/s11756-013-0285-0
n15:organizacniJednotka
12520