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Statements

Subject Item
n2:RIV%2F60076658%3A12520%2F14%3A43886876%21RIV15-MSM-12520___
rdf:type
n4:Vysledek skos:Concept
rdfs:seeAlso
http://www.tandfonline.com/doi/abs/10.1080/00207160.2013.851379#.VKqbbntlw80
dcterms:description
If we are observing very small objects in the microscope the information appears somehow distorted. This distortion is called point spread function (PSF) and it can be measured and simulated as we show in this article. Many experiments and simulations were done in the field of confocal or deconvolution microscopy, but we want to present 3D reconstruction and measurement of PSF in the bright field (or phase contrast) microscopy. Our goal is to use this knowledge about PSF to estimate objective focus. We want to use this method for automatic focusing of the microscope. We measured the PSF on our microscope in bright field using a 100xobjective. We used 15 nm gold and 200 nm latex particles as point sources. We used two different simulations; first one was Airy disc simulation; the second one was Extended Nijboer-Zernike theory. Both approaches were used for description of PSF and finding the position of the focus. If we are observing very small objects in the microscope the information appears somehow distorted. This distortion is called point spread function (PSF) and it can be measured and simulated as we show in this article. Many experiments and simulations were done in the field of confocal or deconvolution microscopy, but we want to present 3D reconstruction and measurement of PSF in the bright field (or phase contrast) microscopy. Our goal is to use this knowledge about PSF to estimate objective focus. We want to use this method for automatic focusing of the microscope. We measured the PSF on our microscope in bright field using a 100xobjective. We used 15 nm gold and 200 nm latex particles as point sources. We used two different simulations; first one was Airy disc simulation; the second one was Extended Nijboer-Zernike theory. Both approaches were used for description of PSF and finding the position of the focus.
dcterms:title
Microscope point spread function, focus and calculation of optimal microscope set-up Microscope point spread function, focus and calculation of optimal microscope set-up
skos:prefLabel
Microscope point spread function, focus and calculation of optimal microscope set-up Microscope point spread function, focus and calculation of optimal microscope set-up
skos:notation
RIV/60076658:12520/14:43886876!RIV15-MSM-12520___
n3:aktivita
n17:S n17:P
n3:aktivity
P(ED2.1.00/01.0024), S
n3:cisloPeriodika
2
n3:dodaniDat
n5:2015
n3:domaciTvurceVysledku
n9:5455316 n9:6003524
n3:druhVysledku
n19:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
29220
n3:idVysledku
RIV/60076658:12520/14:43886876
n3:jazykVysledku
n20:eng
n3:klicovaSlova
Airy disc; bright field microscopy; point spread function; focus; ENZ theory
n3:klicoveSlovo
n6:focus n6:ENZ%20theory n6:bright%20field%20microscopy n6:Airy%20disc n6:point%20spread%20function
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[0F274F745303]
n3:nazevZdroje
INTERNATIONAL JOURNAL OF COMPUTER MATHEMATICS
n3:obor
n14:BO
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n11:ED2.1.00%2F01.0024
n3:rokUplatneniVysledku
n5:2014
n3:svazekPeriodika
91
n3:tvurceVysledku
Štys, Dalibor Náhlík, Tomáš
n3:wos
000335089900006
s:issn
0020-7160
s:numberOfPages
12
n13:doi
10.1080/00207160.2013.851379
n16:organizacniJednotka
12520