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Statements

Subject Item
n2:RIV%2F60076658%3A12520%2F14%3A43886850%21RIV15-GA0-12520___
rdf:type
n13:Vysledek skos:Concept
rdfs:seeAlso
http://www.agriculturejournals.cz/publicFiles/129017.pdf
dcterms:description
Chromosomal manipulations in sturgeons, particularly gynogenesis, are interesting due to the potential to change female ratio in progeny that can be useful for caviar production. The optimization of UV treatment for induction of gynogenesis is complicated due to high and variable optical density of the milt due to differential spermatozoa concentration, and because of sensitivity of spermatozoa's motility apparatus. Therefore in this study we compared chemical methods of sperm treatment as an alternative to short wave-length UV treatment; evaluation considers impact on spermatozoa motility, DNA integrity, and efficiency of DNA inactivation. Dimethyl sulfate (DMS) in concentrations of 2.5-30mM was applied to spermatozoa in order to inactivate DNA. Also ethidium bromide (EB), psoralen (PS), and 4'-aminomethyl-4,5',8-trimethylpsoralen (AMT) were used to increase sensitivity of spermatozoa's DNA to long wavelength UV-A light (360 nm). CASA analyses of treated sperm showed strong negative effects on spermatozoa motility with the increasing concentration of active substances. Additionally in case of PS, EB, and DMS treatment comet assay did not reveal significant DNA damage of sperm at the range of concentrations relatively safe for spermatozoa motility. Flow cytometric analysis of relative DNA content in larvae resulting from activation of normal ova of sterlet with the treated sperm showed low efficiency of haploid gynogenesis induction. The putative gynogenetic larvae were found after treatment with PS in concentrations higher than 18mýM and EB higher than 10mýM followed by UV-A irradiation at the dose of 900 J/m2 and DMS up to 5mM. Chromosomal manipulations in sturgeons, particularly gynogenesis, are interesting due to the potential to change female ratio in progeny that can be useful for caviar production. The optimization of UV treatment for induction of gynogenesis is complicated due to high and variable optical density of the milt due to differential spermatozoa concentration, and because of sensitivity of spermatozoa's motility apparatus. Therefore in this study we compared chemical methods of sperm treatment as an alternative to short wave-length UV treatment; evaluation considers impact on spermatozoa motility, DNA integrity, and efficiency of DNA inactivation. Dimethyl sulfate (DMS) in concentrations of 2.5-30mM was applied to spermatozoa in order to inactivate DNA. Also ethidium bromide (EB), psoralen (PS), and 4'-aminomethyl-4,5',8-trimethylpsoralen (AMT) were used to increase sensitivity of spermatozoa's DNA to long wavelength UV-A light (360 nm). CASA analyses of treated sperm showed strong negative effects on spermatozoa motility with the increasing concentration of active substances. Additionally in case of PS, EB, and DMS treatment comet assay did not reveal significant DNA damage of sperm at the range of concentrations relatively safe for spermatozoa motility. Flow cytometric analysis of relative DNA content in larvae resulting from activation of normal ova of sterlet with the treated sperm showed low efficiency of haploid gynogenesis induction. The putative gynogenetic larvae were found after treatment with PS in concentrations higher than 18mýM and EB higher than 10mýM followed by UV-A irradiation at the dose of 900 J/m2 and DMS up to 5mM.
dcterms:title
Chemical induction of haploid gynogenesis in sterlet Acipenser ruthenus Chemical induction of haploid gynogenesis in sterlet Acipenser ruthenus
skos:prefLabel
Chemical induction of haploid gynogenesis in sterlet Acipenser ruthenus Chemical induction of haploid gynogenesis in sterlet Acipenser ruthenus
skos:notation
RIV/60076658:12520/14:43886850!RIV15-GA0-12520___
n3:aktivita
n4:S n4:P
n3:aktivity
P(ED2.1.00/01.0024), P(GA14-02940S), P(LO1205), S
n3:cisloPeriodika
7
n3:dodaniDat
n14:2015
n3:domaciTvurceVysledku
n6:4098285 n6:4309952 n6:7078935
n3:druhVysledku
n5:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
7085
n3:idVysledku
RIV/60076658:12520/14:43886850
n3:jazykVysledku
n16:eng
n3:klicovaSlova
Sturgeons; Psoralen; Dimethyl sulfate; Comet assay; Chromosomal manipulation
n3:klicoveSlovo
n7:Chromosomal%20manipulation n7:Dimethyl%20sulfate n7:Comet%20assay n7:Psoralen n7:Sturgeons
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[236D5B753EBE]
n3:nazevZdroje
Czech Journal of Animal Science : Živočišná výroba
n3:obor
n15:GL
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n11:ED2.1.00%2F01.0024 n11:LO1205 n11:GA14-02940S
n3:rokUplatneniVysledku
n14:2014
n3:svazekPeriodika
59
n3:tvurceVysledku
Lebeda, Ievgen Flajšhans, Martin Gazo, Ievgenia
s:issn
1212-1819
s:numberOfPages
9
n18:organizacniJednotka
12520