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Statements

Subject Item
n2:RIV%2F60076658%3A12520%2F14%3A43886826%21RIV15-MZE-12520___
rdf:type
skos:Concept n18:Vysledek
rdfs:seeAlso
http://www.agriculturejournals.cz/publishedArticles/CJAS/2014-59-1-1.pdf
dcterms:description
Spermatozoa morphology, ultrastructure, and spermatozoa motility traits were studied in pikeperch (Sander lucioperca) after activation in various media (AM 1 - 45mM NaCl, 5mM KCl, 20mM Tris, pH 8.5; AM 2 - 100mM sucrose, 20mM Tris, pH 8.5; AM 3 - 100mM sucrose, 1mM CaCl2, 20mM Tris, pH 8.5) during a 48-hour storage period. The spermatozoon was acrosomeless and differentiated into a spherical nucleus (head), midpiece, and flagellum. The nucleus length and width measured 1.83 +/- 0.03 and 1.63 +/- 0.02 mm, respectively. The midpiece was located laterally to the nucleus and possessed proximal and distal centrioles and 2-4 mitochondria. Flagellar length was 33.2 +/- 0.90 mu m, and a pair of lateral fin-like structures projections was observed. The axoneme consisted of nine peripheral doublet microtubules and a single central pair. After a 24 h storage in all activation media at all sampling times post-activation (15, 45, 90, and 120 s), spermatozoa motility was significantly decreased. Spermatozoa were motile after the 48-hour storage at all sampling times post-activation only in AM 3. After the 48-hour storage, no motile spermatozoa were observed in AM 2 and AM 1 at 90 and 120 s post-activation, respectively. Differences in spermatozoa velocity varied with activation medium during storage. After the 48-hour storage in AM 1 and AM 2, decrease of spermatozoa velocity at 15 s post-activation was observed, while in AM 3, velocity was decreased only after the 48-hour storage. Pikeperch spermatozoa morphology and ultrastructure was found similar to that of most freshwater teleosts, with differences in the arrangement of midpiece, number of mitochondria, and position of centrioles. Viable pikeperch sperm was observed after the 48-hour storage. Motility of spermatozoa was improved by addition of Ca2+ to the activation medium, where higher spermatozoa velocity was observed. Spermatozoa morphology, ultrastructure, and spermatozoa motility traits were studied in pikeperch (Sander lucioperca) after activation in various media (AM 1 - 45mM NaCl, 5mM KCl, 20mM Tris, pH 8.5; AM 2 - 100mM sucrose, 20mM Tris, pH 8.5; AM 3 - 100mM sucrose, 1mM CaCl2, 20mM Tris, pH 8.5) during a 48-hour storage period. The spermatozoon was acrosomeless and differentiated into a spherical nucleus (head), midpiece, and flagellum. The nucleus length and width measured 1.83 +/- 0.03 and 1.63 +/- 0.02 mm, respectively. The midpiece was located laterally to the nucleus and possessed proximal and distal centrioles and 2-4 mitochondria. Flagellar length was 33.2 +/- 0.90 mu m, and a pair of lateral fin-like structures projections was observed. The axoneme consisted of nine peripheral doublet microtubules and a single central pair. After a 24 h storage in all activation media at all sampling times post-activation (15, 45, 90, and 120 s), spermatozoa motility was significantly decreased. Spermatozoa were motile after the 48-hour storage at all sampling times post-activation only in AM 3. After the 48-hour storage, no motile spermatozoa were observed in AM 2 and AM 1 at 90 and 120 s post-activation, respectively. Differences in spermatozoa velocity varied with activation medium during storage. After the 48-hour storage in AM 1 and AM 2, decrease of spermatozoa velocity at 15 s post-activation was observed, while in AM 3, velocity was decreased only after the 48-hour storage. Pikeperch spermatozoa morphology and ultrastructure was found similar to that of most freshwater teleosts, with differences in the arrangement of midpiece, number of mitochondria, and position of centrioles. Viable pikeperch sperm was observed after the 48-hour storage. Motility of spermatozoa was improved by addition of Ca2+ to the activation medium, where higher spermatozoa velocity was observed.
dcterms:title
Sperm morphology, ultrastructure, and motility in pikeperch Sander lucioperca (Percidae, Teleostei) associated with various activation media Sperm morphology, ultrastructure, and motility in pikeperch Sander lucioperca (Percidae, Teleostei) associated with various activation media
skos:prefLabel
Sperm morphology, ultrastructure, and motility in pikeperch Sander lucioperca (Percidae, Teleostei) associated with various activation media Sperm morphology, ultrastructure, and motility in pikeperch Sander lucioperca (Percidae, Teleostei) associated with various activation media
skos:notation
RIV/60076658:12520/14:43886826!RIV15-MZE-12520___
n3:aktivita
n11:S n11:P
n3:aktivity
P(ED2.1.00/01.0024), P(GAP503/12/1834), P(LO1205), P(QI101C033), S
n3:cisloPeriodika
1
n3:dodaniDat
n5:2015
n3:domaciTvurceVysledku
n10:6919707 n10:5033438 Hatef, Azadeh n10:1799630
n3:druhVysledku
n14:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
46622
n3:idVysledku
RIV/60076658:12520/14:43886826
n3:jazykVysledku
n7:eng
n3:klicovaSlova
storage time; calcium; spermatozoa velocity; spermatozoa motility
n3:klicoveSlovo
n8:calcium n8:spermatozoa%20motility n8:spermatozoa%20velocity n8:storage%20time
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[DA19DA50FECC]
n3:nazevZdroje
Czech Journal of Animal Science : Živočišná výroba
n3:obor
n4:GL
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n6:QI101C033 n6:LO1205 n6:ED2.1.00%2F01.0024 n6:GAP503%2F12%2F1834
n3:rokUplatneniVysledku
n5:2014
n3:svazekPeriodika
59
n3:tvurceVysledku
Hatef, Azadeh Policar, Tomáš Křišťan, Jiří Alavi, Sayyed Mohammad Hadi
n3:wos
000335888900001
s:issn
1212-1819
s:numberOfPages
10
n19:organizacniJednotka
12520