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Statements

Subject Item
n2:RIV%2F60076658%3A12520%2F14%3A43886818%21RIV15-GA0-12520___
rdf:type
skos:Concept n12:Vysledek
rdfs:seeAlso
http://link.springer.com/article/10.1007%2Fs10695-014-9936-5
dcterms:description
The role of environmental ion composition and osmolality in Ca2+ signaled activation was assessed in spermatozoa of brook trout Salvelinus fontinalis. Milt from ten mature males was obtained by abdominal massage. Spermatozoa motility was evaluated in 0, 100, and 300 mOsm/kg NaCl or sucrose solutions, buffered by 10 mM Tris-HCl pH 8.5. For investigation of spermatozoa reaction to external Ca2+ concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator. For investigation of the effect of external Na+ concentration in conditions of low external Ca2+, 100 A mu M amiloride was added to the EGTA-containing solutions as a Na+ transport blocker. Low motility was observed in sucrose (Na+ free) solutions containing 2 mM EGTA but not in Na+ solutions containing 2 mM EGTA. Addition of amiloride led to significantly increased motility (P { 0.05) compared with sucrose (Na+ free) solutions containing 2 mM EGTA. We conclude that Na+ transport in Ca2+-free solutions plays a regulatory role in brook trout spermatozoa activation. The influence of competitive Na+ and Ca2+ transport on the control of spermatozoa activation requires further study with respect to its application for improvement of artificial activation and storage media. The role of environmental ion composition and osmolality in Ca2+ signaled activation was assessed in spermatozoa of brook trout Salvelinus fontinalis. Milt from ten mature males was obtained by abdominal massage. Spermatozoa motility was evaluated in 0, 100, and 300 mOsm/kg NaCl or sucrose solutions, buffered by 10 mM Tris-HCl pH 8.5. For investigation of spermatozoa reaction to external Ca2+ concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator. For investigation of the effect of external Na+ concentration in conditions of low external Ca2+, 100 A mu M amiloride was added to the EGTA-containing solutions as a Na+ transport blocker. Low motility was observed in sucrose (Na+ free) solutions containing 2 mM EGTA but not in Na+ solutions containing 2 mM EGTA. Addition of amiloride led to significantly increased motility (P { 0.05) compared with sucrose (Na+ free) solutions containing 2 mM EGTA. We conclude that Na+ transport in Ca2+-free solutions plays a regulatory role in brook trout spermatozoa activation. The influence of competitive Na+ and Ca2+ transport on the control of spermatozoa activation requires further study with respect to its application for improvement of artificial activation and storage media.
dcterms:title
The role of Ca2+ and Na+ membrane transport in brook trout (Salvelinus fontinalis) spermatozoa motility The role of Ca2+ and Na+ membrane transport in brook trout (Salvelinus fontinalis) spermatozoa motility
skos:prefLabel
The role of Ca2+ and Na+ membrane transport in brook trout (Salvelinus fontinalis) spermatozoa motility The role of Ca2+ and Na+ membrane transport in brook trout (Salvelinus fontinalis) spermatozoa motility
skos:notation
RIV/60076658:12520/14:43886818!RIV15-GA0-12520___
n3:aktivita
n8:S n8:P
n3:aktivity
P(ED2.1.00/01.0024), P(EE2.3.20.0024), P(GAP502/11/0090), P(GAP502/12/1973), P(LO1205), S
n3:cisloPeriodika
5
n3:dodaniDat
n15:2015
n3:domaciTvurceVysledku
Dzyuba, Boris n16:1593579 Cosson, Jacky n16:1144502 n16:5876001
n3:druhVysledku
n10:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n7:predkladatel
n3:idSjednocenehoVysledku
43088
n3:idVysledku
RIV/60076658:12520/14:43886818
n3:jazykVysledku
n20:eng
n3:klicovaSlova
Salmonid fish; Amiloride; Ion transport; Osmolality; Sperm motility activation
n3:klicoveSlovo
n4:Sperm%20motility%20activation n4:Ion%20transport n4:Osmolality n4:Salmonid%20fish n4:Amiloride
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[BD449CC3758F]
n3:nazevZdroje
Fish Physiology and Biochemistry
n3:obor
n14:GL
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n6:EE2.3.20.0024 n6:ED2.1.00%2F01.0024 n6:GAP502%2F11%2F0090 n6:GAP502%2F12%2F1973 n6:LO1205
n3:rokUplatneniVysledku
n15:2014
n3:svazekPeriodika
40
n3:tvurceVysledku
Rodina, Marek Cosson, Jacky Bondarenko, Olga Dzyuba, Boris Linhart, Otomar
n3:wos
000341498900011
s:issn
0920-1742
s:numberOfPages
5
n17:doi
10.1007/s10695-014-9936-5
n19:organizacniJednotka
12520