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Statements

Subject Item
n2:RIV%2F60076658%3A12520%2F13%3A43885205%21RIV14-GA0-12520___
rdf:type
n6:Vysledek skos:Concept
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S0093691X13000083
dcterms:description
The aim of this study was to describe spermatozoa volume changes during the motility period of fish species with either osmotic (common carp Cyprinus carpio) or with ionic (sterlet Acipenseri ruthenus and brook trout Salvelinus fontinalis) modes of motility activation. Nephelometry, light microscopy, and spermatocrit methods were used for quantitative assessment of cell volume changes in media of different osmolalities. Significant correlation (R-2 = 0.7341; P { 0.001) between parameter of volume changes measured using nephelometry and light microscopy methods confirmed nephelometry as a sufficiently sensitive method to detect changes of spermatozoa volume. The spermatocrit alteration method resulted in a large proportion of damaged and potentially immotile spermatozoa in media of osmolality less than 150 mOsm/kg in carp and osmolalities from 10 to 300 mOsm/kg in sterlet and brook trout. Therefore, this method is not reliable for assessing spermatozoa swelling in hypotonic solutions, because the integrity of the cells is not fully preserved. Increase in carp spermatozoa (osmotic activation mode) volume occurred during the motility period in hypotonic conditions, but no indications of volume changes were found in sterlet and brook trout spermatozoa (ionic activation mode) associated with environmental osmolality alteration. Accordingly, we conclude that sperm volume changes are differentially involved in the motility activation process. Species-specific differences in spermatozoa volume changes as a response to a hypotonic environment during the motility period are discussed in relation to their potent physiological role. The aim of this study was to describe spermatozoa volume changes during the motility period of fish species with either osmotic (common carp Cyprinus carpio) or with ionic (sterlet Acipenseri ruthenus and brook trout Salvelinus fontinalis) modes of motility activation. Nephelometry, light microscopy, and spermatocrit methods were used for quantitative assessment of cell volume changes in media of different osmolalities. Significant correlation (R-2 = 0.7341; P { 0.001) between parameter of volume changes measured using nephelometry and light microscopy methods confirmed nephelometry as a sufficiently sensitive method to detect changes of spermatozoa volume. The spermatocrit alteration method resulted in a large proportion of damaged and potentially immotile spermatozoa in media of osmolality less than 150 mOsm/kg in carp and osmolalities from 10 to 300 mOsm/kg in sterlet and brook trout. Therefore, this method is not reliable for assessing spermatozoa swelling in hypotonic solutions, because the integrity of the cells is not fully preserved. Increase in carp spermatozoa (osmotic activation mode) volume occurred during the motility period in hypotonic conditions, but no indications of volume changes were found in sterlet and brook trout spermatozoa (ionic activation mode) associated with environmental osmolality alteration. Accordingly, we conclude that sperm volume changes are differentially involved in the motility activation process. Species-specific differences in spermatozoa volume changes as a response to a hypotonic environment during the motility period are discussed in relation to their potent physiological role.
dcterms:title
Volume changes during the motility period of fish spermatozoa: Interspecies differences Volume changes during the motility period of fish spermatozoa: Interspecies differences
skos:prefLabel
Volume changes during the motility period of fish spermatozoa: Interspecies differences Volume changes during the motility period of fish spermatozoa: Interspecies differences
skos:notation
RIV/60076658:12520/13:43885205!RIV14-GA0-12520___
n6:predkladatel
n7:orjk%3A12520
n3:aktivita
n21:S n21:P
n3:aktivity
P(ED2.1.00/01.0024), P(GAP502/11/0090), P(GAP502/12/1973), P(GPP502/10/P426), S
n3:cisloPeriodika
5
n3:dodaniDat
n12:2014
n3:domaciTvurceVysledku
n14:1144502 Dzyuba, Boris Prokopchuk, Galina n14:9895744 n14:1593579 Cosson, Jacky
n3:druhVysledku
n4:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
115077
n3:idVysledku
RIV/60076658:12520/13:43885205
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Volume changes; Osmolality; Motility activation; Fish sperm
n3:klicoveSlovo
n5:Fish%20sperm n5:Osmolality n5:Motility%20activation n5:Volume%20changes
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[75A2F42B7A56]
n3:nazevZdroje
Theriogenology
n3:obor
n18:GL
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
7
n3:projekt
n10:ED2.1.00%2F01.0024 n10:GAP502%2F11%2F0090 n10:GPP502%2F10%2FP426 n10:GAP502%2F12%2F1973
n3:rokUplatneniVysledku
n12:2013
n3:svazekPeriodika
79
n3:tvurceVysledku
Yamaner, Gunes Prokopchuk, Galina Linhart, Otomar Cosson, Jacky Bondarenko, Olga Dzyuba, Boris Pšenička, Martin
n3:wos
000315760000020
s:issn
0093-691X
s:numberOfPages
10
n15:doi
10.1016/j.theriogenology.2013.01.005
n20:organizacniJednotka
12520