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Statements

Subject Item
n2:RIV%2F60076658%3A12520%2F13%3A43885204%21RIV14-MZE-12520___
rdf:type
n10:Vysledek skos:Concept
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S0011224012002738
dcterms:description
Post-thaw motility rate, curvilinear velocity (VCL), and fertilizing ability of carp spermatozoa can be improved by short-term treatment with moderately hypotonic media prior to freezing. Before cryopreservation, carp sperm samples were treated with NaCl solutions of differing osmolalities, ranging from 100 to 300 mOsm kg(-1) for 10 s, after which final osmolality was adjusted to 300 mOsm kg(-1). The resulting sperm suspension was diluted 1:1 with cryoprotective medium and frozen using conventional techniques. Control samples were treated in the same way, without the pre-dilution step. Post-thaw motility rate in samples pretreated with 200 mOsm kg(1) NaCl was significantly higher (44 +/- 10%) than in controls (21 +/- 15%) and samples pretreated with 100 mOsm kg(-1) (25 +/- 15%) and 300 mOsm kg(-1) (25 +/- 12%) NaCl. Significantly higher mean VCL were observed in samples pretreated with 100, 150, and 200 mOsm kg(-1) (119 +/- 24, 118 +/- 22, and 115 +/- 32 mu m s(-1), respectively) compared to controls (92 27 mu m s-1). Fertilization rate of frozen-thawed sperm treated with 200 mOsm kg(-1) solution of 2 M NaCl was significantly higher (25 +/- 18%) than that of sperm treated with 300 mOsm kg(-1) NaCl solutions (12 +/- 7%) and the control (9 +/- 6%). Post-thaw motility rate, curvilinear velocity (VCL), and fertilizing ability of carp spermatozoa can be improved by short-term treatment with moderately hypotonic media prior to freezing. Before cryopreservation, carp sperm samples were treated with NaCl solutions of differing osmolalities, ranging from 100 to 300 mOsm kg(-1) for 10 s, after which final osmolality was adjusted to 300 mOsm kg(-1). The resulting sperm suspension was diluted 1:1 with cryoprotective medium and frozen using conventional techniques. Control samples were treated in the same way, without the pre-dilution step. Post-thaw motility rate in samples pretreated with 200 mOsm kg(1) NaCl was significantly higher (44 +/- 10%) than in controls (21 +/- 15%) and samples pretreated with 100 mOsm kg(-1) (25 +/- 15%) and 300 mOsm kg(-1) (25 +/- 12%) NaCl. Significantly higher mean VCL were observed in samples pretreated with 100, 150, and 200 mOsm kg(-1) (119 +/- 24, 118 +/- 22, and 115 +/- 32 mu m s(-1), respectively) compared to controls (92 27 mu m s-1). Fertilization rate of frozen-thawed sperm treated with 200 mOsm kg(-1) solution of 2 M NaCl was significantly higher (25 +/- 18%) than that of sperm treated with 300 mOsm kg(-1) NaCl solutions (12 +/- 7%) and the control (9 +/- 6%).
dcterms:title
Hypotonic treatment prior to freezing improves cryoresistance of common carp (Cyprinus carpio L.) spermatozoa Hypotonic treatment prior to freezing improves cryoresistance of common carp (Cyprinus carpio L.) spermatozoa
skos:prefLabel
Hypotonic treatment prior to freezing improves cryoresistance of common carp (Cyprinus carpio L.) spermatozoa Hypotonic treatment prior to freezing improves cryoresistance of common carp (Cyprinus carpio L.) spermatozoa
skos:notation
RIV/60076658:12520/13:43885204!RIV14-MZE-12520___
n10:predkladatel
n11:orjk%3A12520
n3:aktivita
n20:S n20:P
n3:aktivity
P(ED2.1.00/01.0024), P(GAP502/11/0090), P(IAA608030801), P(LC06073), P(ME10015), P(QH82119), S
n3:cisloPeriodika
2
n3:dodaniDat
n16:2014
n3:domaciTvurceVysledku
Shaliutina, Anna Cosson, Jacky n14:5876001 n14:3624900 n14:1144502 n14:7643853 Dzyuba, Boris n14:1593579
n3:druhVysledku
n18:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
78572
n3:idVysledku
RIV/60076658:12520/13:43885204
n3:jazykVysledku
n21:eng
n3:klicovaSlova
Cryoresistance; Hypotonic treatment; Carp sperm
n3:klicoveSlovo
n9:Carp%20sperm n9:Hypotonic%20treatment n9:Cryoresistance
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[C5DF712F0576]
n3:nazevZdroje
Cryobiology
n3:obor
n12:GL
n3:pocetDomacichTvurcuVysledku
8
n3:pocetTvurcuVysledku
9
n3:projekt
n4:QH82119 n4:IAA608030801 n4:LC06073 n4:GAP502%2F11%2F0090 n4:ED2.1.00%2F01.0024 n4:ME10015
n3:rokUplatneniVysledku
n16:2013
n3:svazekPeriodika
66
n3:tvurceVysledku
Rodina, Marek Linhart, Otomar Yamaner, Gunes Shaliutina, Anna Bondarenko, Volodymyr Gela, David Cosson, Jacky Bondarenko, Olga Dzyuba, Boris
n3:wos
000316518100018
s:issn
0011-2240
s:numberOfPages
3
n17:doi
10.1016/j.cryobiol.2012.12.003
n5:organizacniJednotka
12520