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Statements

Subject Item
n2:RIV%2F60076658%3A12520%2F13%3A43885198%21RIV14-MZE-12520___
rdf:type
n6:Vysledek skos:Concept
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S0378432013000936
dcterms:description
The sperm of Russian sturgeon (A. gueldenstaedtii) and Siberian sturgeon (A. baerii was used to evaluate the effects of short-term (liquid) storage on functional parameters (spermatozoa motility and velocity), DNA integrity and oxidative stress indices. Spermatozoa showed }50% motility during 6 days of storage with an average velocity of 133.12 +/- 15.4 to 87.9 +/- 11.23 mu m s(-1) in both species. No motile spermatozoa were recorded after nine days of storage. Analysis of Russian sturgeon sperm showed no significant differences in DNA damage expressed as percent tail DNA and Olive Tail Moment for first three days of storage. In Siberian sturgeon significant differences in DNA damage were detected after two days of storage. The level of oxidative stress indices (TSARS, CP) and antioxidant activity (SOD) increased significantly with storage time in both species. Results of this study can be utilized for successful reproduction management and cryopreservation protocols of these endangered species. The sperm of Russian sturgeon (A. gueldenstaedtii) and Siberian sturgeon (A. baerii was used to evaluate the effects of short-term (liquid) storage on functional parameters (spermatozoa motility and velocity), DNA integrity and oxidative stress indices. Spermatozoa showed }50% motility during 6 days of storage with an average velocity of 133.12 +/- 15.4 to 87.9 +/- 11.23 mu m s(-1) in both species. No motile spermatozoa were recorded after nine days of storage. Analysis of Russian sturgeon sperm showed no significant differences in DNA damage expressed as percent tail DNA and Olive Tail Moment for first three days of storage. In Siberian sturgeon significant differences in DNA damage were detected after two days of storage. The level of oxidative stress indices (TSARS, CP) and antioxidant activity (SOD) increased significantly with storage time in both species. Results of this study can be utilized for successful reproduction management and cryopreservation protocols of these endangered species.
dcterms:title
Effect of short-term storage on quality parameters, DNA integrity, and oxidative stress in Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) sturgeon sperm Effect of short-term storage on quality parameters, DNA integrity, and oxidative stress in Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) sturgeon sperm
skos:prefLabel
Effect of short-term storage on quality parameters, DNA integrity, and oxidative stress in Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) sturgeon sperm Effect of short-term storage on quality parameters, DNA integrity, and oxidative stress in Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) sturgeon sperm
skos:notation
RIV/60076658:12520/13:43885198!RIV14-MZE-12520___
n6:predkladatel
n15:orjk%3A12520
n3:aktivita
n4:S n4:P
n3:aktivity
P(ED2.1.00/01.0024), P(GAP502/11/0090), P(IAA608030801), P(ME10015), P(QH82119), S
n3:cisloPeriodika
1-4
n3:dodaniDat
n8:2014
n3:domaciTvurceVysledku
n5:2489627 Shaliutina, Anna Gazo, Ievgenia n5:1097253 n5:1593579
n3:druhVysledku
n20:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
71734
n3:idVysledku
RIV/60076658:12520/13:43885198
n3:jazykVysledku
n18:eng
n3:klicovaSlova
DNA integrity; Spermatozoa motility; Comet assay; Oxidative stress; Sperm storage
n3:klicoveSlovo
n7:Sperm%20storage n7:Spermatozoa%20motility n7:DNA%20integrity n7:Oxidative%20stress n7:Comet%20assay
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[790494F000C6]
n3:nazevZdroje
Animal Reproduction Science
n3:obor
n19:GL
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n9:ED2.1.00%2F01.0024 n9:GAP502%2F11%2F0090 n9:QH82119 n9:IAA608030801 n9:ME10015
n3:rokUplatneniVysledku
n8:2013
n3:svazekPeriodika
139
n3:tvurceVysledku
Linhart, Otomar Shaliutina, Anna Gazo, Ievgenia Linhartová, Pavla Hulák, Martin
n3:wos
000320493100018
s:issn
0378-4320
s:numberOfPages
9
n17:doi
10.1016/j.anireprosci.2013.03.006
n16:organizacniJednotka
12520