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Statements

Subject Item
n2:RIV%2F60076658%3A12520%2F13%3A43883414%21RIV14-MZE-12520___
rdf:type
n5:Vysledek skos:Concept
rdfs:seeAlso
http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0531.2012.02118.x/abstract;jsessionid=010AECFBFCB18BC48FBF70CCA17E0A80.f01t02
dcterms:description
Seminal plasma of sterlet Acipenser ruthenus was evaluated using comparative proteomics to characterize its protein fractions and to determine any influence of multiple sperm collections on these proteins. An experimental group of fish was used, in which sperm was collected three times at 5 h intervals. Protein fractions of seminal plasma were determined by SDS-gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis high-resolution gels (2D). At all stripping times, five protein bands with molecular weights of 93, 53, 48, 33 and 28 kDa were identified using SDS-PAGE. No significant differences (p } 0.05) in relative mass of protein bands among collections were observed. At the third collection, 20 protein spots were detected from the two-dimensional gels, compared to 17 found at the first and second collections. Ten protein spots, from the third stripping, were analysed. Screening of these spots by mass spectrometric analysis showed positive results for spot 10. Direct comparison across public databases revealed sequence similarity with two hypothetical proteins, MCAG_00854 and IscW_ISCW011489. Differences in the seminal plasma protein fractions were found at the third stripping compared to the first two. It is hypothesized that these extra proteins after the third collection could be involved in some step of intracellular mechanism which is responsible for regulating of spermatozoa motility. However, protein identification revealed no significant distinction for any protein spot and protein sequences available in public databases. These results highlighted the need for a complete genome sequences for sturgeons. Seminal plasma of sterlet Acipenser ruthenus was evaluated using comparative proteomics to characterize its protein fractions and to determine any influence of multiple sperm collections on these proteins. An experimental group of fish was used, in which sperm was collected three times at 5 h intervals. Protein fractions of seminal plasma were determined by SDS-gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis high-resolution gels (2D). At all stripping times, five protein bands with molecular weights of 93, 53, 48, 33 and 28 kDa were identified using SDS-PAGE. No significant differences (p } 0.05) in relative mass of protein bands among collections were observed. At the third collection, 20 protein spots were detected from the two-dimensional gels, compared to 17 found at the first and second collections. Ten protein spots, from the third stripping, were analysed. Screening of these spots by mass spectrometric analysis showed positive results for spot 10. Direct comparison across public databases revealed sequence similarity with two hypothetical proteins, MCAG_00854 and IscW_ISCW011489. Differences in the seminal plasma protein fractions were found at the third stripping compared to the first two. It is hypothesized that these extra proteins after the third collection could be involved in some step of intracellular mechanism which is responsible for regulating of spermatozoa motility. However, protein identification revealed no significant distinction for any protein spot and protein sequences available in public databases. These results highlighted the need for a complete genome sequences for sturgeons.
dcterms:title
Comparison of Protein Fractions in Seminal Plasma from Multiple Sperm Collections in Sterlet (Acipenser ruthenus) Comparison of Protein Fractions in Seminal Plasma from Multiple Sperm Collections in Sterlet (Acipenser ruthenus)
skos:prefLabel
Comparison of Protein Fractions in Seminal Plasma from Multiple Sperm Collections in Sterlet (Acipenser ruthenus) Comparison of Protein Fractions in Seminal Plasma from Multiple Sperm Collections in Sterlet (Acipenser ruthenus)
skos:notation
RIV/60076658:12520/13:43883414!RIV14-MZE-12520___
n5:predkladatel
n6:orjk%3A12520
n3:aktivita
n16:S n16:P n16:I
n3:aktivity
I, P(ED2.1.00/01.0024), P(GAP502/11/0090), P(IAA608030801), P(KJB608030901), P(ME10015), P(QH82118), P(QH82119), P(QH92308), S
n3:cisloPeriodika
1
n3:dodaniDat
n20:2014
n3:domaciTvurceVysledku
n4:2489627 Shaliutina, Anna n4:1593579 Dzyuba, Boris Li, Ping
n3:druhVysledku
n17:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n21:predkladatel
n3:idSjednocenehoVysledku
66286
n3:idVysledku
RIV/60076658:12520/13:43883414
n3:jazykVysledku
n19:eng
n3:klicovaSlova
sterlet; sperm; plasma; seminal; Protein
n3:klicoveSlovo
n14:sterlet n14:seminal n14:sperm n14:plasma n14:Protein
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[295858506A68]
n3:nazevZdroje
Reproduction in Domestic Animals
n3:obor
n9:GL
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:projekt
n10:GAP502%2F11%2F0090 n10:QH82118 n10:ED2.1.00%2F01.0024 n10:ME10015 n10:KJB608030901 n10:QH92308 n10:IAA608030801 n10:QH82119
n3:rokUplatneniVysledku
n20:2013
n3:svazekPeriodika
48
n3:tvurceVysledku
Hulák, Martin Linhart, Otomar Shaliutina, Anna Dzyuba, Boris Šulc, M. Li, Ping
n3:wos
000313875800027
s:issn
0936-6768
s:numberOfPages
4
n18:doi
10.1111/j.1439-0531.2012.02118.x
n11:organizacniJednotka
12520