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Statements

Subject Item
n2:RIV%2F60076658%3A12520%2F12%3A43883477%21RIV13-MSM-12520___
rdf:type
skos:Concept n9:Vysledek
dcterms:description
Motility of Pinctada margaritifera (Linnaeus, 1758); var: cumingii (Jameson, 1901) (P. margaritifera) spermatozoa collected from gonads are not immediately activated at spawning in seawater (SW) but motility occurs when spermatozoa are transferred into alkaline seawater (pH ranging from 9.0 to 11.4). This motility-activating effect of alkaline pH is reversed when pH is shifted back to more acidic values. In both cases, activity of sperm (% motile cells) increases gradually after alkaline pH activation then lasts for several minutes. The characteristics of these fully motile spermatozoa are described in details at the level of flagella: the wave amplitude and wave-length range 5 to 6 mu m and 15 mu m respectively, while the flagellar beat frequency is approximately 49 Hz. The velocity of sperm displacement is from 220 to 230 mu m/sec. The general swimming pattern is almost circular: the head trajectories describe portions of circles intercalated with small linear segments. Spermatozoa saved in natural seawater at 4 degrees C retain potent motility for several days and can be subsequently activated by alkaline seawater. Respiration and ATP concentration were measured in 3 conditions: regular seawater (pH 7.8), artificial diluent (pH 8.2), and alkaline Tris-buffered seawater (pH 10.5). Results show that sperm respiration rates are higher whereas ATP levels are lower in the latter two media. Motility of Pinctada margaritifera (Linnaeus, 1758); var: cumingii (Jameson, 1901) (P. margaritifera) spermatozoa collected from gonads are not immediately activated at spawning in seawater (SW) but motility occurs when spermatozoa are transferred into alkaline seawater (pH ranging from 9.0 to 11.4). This motility-activating effect of alkaline pH is reversed when pH is shifted back to more acidic values. In both cases, activity of sperm (% motile cells) increases gradually after alkaline pH activation then lasts for several minutes. The characteristics of these fully motile spermatozoa are described in details at the level of flagella: the wave amplitude and wave-length range 5 to 6 mu m and 15 mu m respectively, while the flagellar beat frequency is approximately 49 Hz. The velocity of sperm displacement is from 220 to 230 mu m/sec. The general swimming pattern is almost circular: the head trajectories describe portions of circles intercalated with small linear segments. Spermatozoa saved in natural seawater at 4 degrees C retain potent motility for several days and can be subsequently activated by alkaline seawater. Respiration and ATP concentration were measured in 3 conditions: regular seawater (pH 7.8), artificial diluent (pH 8.2), and alkaline Tris-buffered seawater (pH 10.5). Results show that sperm respiration rates are higher whereas ATP levels are lower in the latter two media.
dcterms:title
Motility activation and metabolism characteristics of spermatozoa of the black-lip-pearl oyster Pinctada margaritifera var: cumingii (Jameson, 1901) Motility activation and metabolism characteristics of spermatozoa of the black-lip-pearl oyster Pinctada margaritifera var: cumingii (Jameson, 1901)
skos:prefLabel
Motility activation and metabolism characteristics of spermatozoa of the black-lip-pearl oyster Pinctada margaritifera var: cumingii (Jameson, 1901) Motility activation and metabolism characteristics of spermatozoa of the black-lip-pearl oyster Pinctada margaritifera var: cumingii (Jameson, 1901)
skos:notation
RIV/60076658:12520/12:43883477!RIV13-MSM-12520___
n9:predkladatel
n10:orjk%3A12520
n4:aktivita
n15:P
n4:aktivity
P(ED2.1.00/01.0024)
n4:cisloPeriodika
1
n4:dodaniDat
n13:2013
n4:domaciTvurceVysledku
Cosson, Jacky
n4:druhVysledku
n8:J
n4:duvernostUdaju
n5:S
n4:entitaPredkladatele
n14:predkladatel
n4:idSjednocenehoVysledku
151898
n4:idVysledku
RIV/60076658:12520/12:43883477
n4:jazykVysledku
n16:eng
n4:klicovaSlova
Sperm energetics; Motility parameters; Sperm quality; Pinctada margaritifera; Pearl oyster
n4:klicoveSlovo
n11:Motility%20parameters n11:Pearl%20oyster n11:Sperm%20quality n11:Sperm%20energetics n11:Pinctada%20margaritifera
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[5AE56C887694]
n4:nazevZdroje
Theriogenology
n4:obor
n19:GL
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
5
n4:projekt
n6:ED2.1.00%2F01.0024
n4:rokUplatneniVysledku
n13:2012
n4:svazekPeriodika
77
n4:tvurceVysledku
Cosson, Jacky Demoy-Schneider, M. Leveque, A. Schmitt, N. Le Pennec, M.
n4:wos
000298336300007
s:issn
0093-691X
s:numberOfPages
12
n18:doi
10.1016/j.theriogenology.2011.07.014
n17:organizacniJednotka
12520