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Statements

Subject Item
n2:RIV%2F60076658%3A12520%2F12%3A43883408%21RIV13-MZE-12520___
rdf:type
skos:Concept n15:Vysledek
dcterms:description
We describe spermatozoa characteristics from sequential collections in sterlet, Acipenser ruthenus, following a single dose of carp pituitary extract (CPE). Sperm production and spermatozoa fertilizing ability, percent motility, and curvilinear velocity (VCL) were investigated in fresh and frozen/thawed sperm. Sperm was collected by two procedures: (A) stripping 3 times per 24 h at 3 h intervals on 3 consecutive days beginning 12 h after CPE treatment, and (B) stripping 3 times over 6 h beginning 36 h after CPE treatment. Spermatozoa motility and VCL were evaluated by video microscopy, and sperm production was measured as volume and concentration. Sperm samples were frozen by a conventional freezing procedure in a cryoprotective medium containing 10% methanol. Fertilization was conducted using a ratio of 105 spermatozoa/egg. Both sequential stripping procedures yielded larger volumes of viable spermatozoa than did a single collection. Sperm parameters such as density and volume varied widely depending on collection time. The highest numbers of spermatozoa per individual were collected 15-42 h post-CPE treatment in (A) and at 42 h post-CPE treatment in (B) (85+/-4% and 64+/-5% of total spermatozoa count, respectively). Median percent motility in spermatozoa before cryopreservation was 26-100% and 5-67% post-thaw. Fertilization rates obtained with frozen/thawed spermatozoa were 13-76% (median value). A significant increase in spermatozoa motility parameters and fertilizing ability at the second collection on each day was observed. Sequential stripping and spermatozoa cryopreservation in combination could improve the efficiency of sturgeon aquaculture. We describe spermatozoa characteristics from sequential collections in sterlet, Acipenser ruthenus, following a single dose of carp pituitary extract (CPE). Sperm production and spermatozoa fertilizing ability, percent motility, and curvilinear velocity (VCL) were investigated in fresh and frozen/thawed sperm. Sperm was collected by two procedures: (A) stripping 3 times per 24 h at 3 h intervals on 3 consecutive days beginning 12 h after CPE treatment, and (B) stripping 3 times over 6 h beginning 36 h after CPE treatment. Spermatozoa motility and VCL were evaluated by video microscopy, and sperm production was measured as volume and concentration. Sperm samples were frozen by a conventional freezing procedure in a cryoprotective medium containing 10% methanol. Fertilization was conducted using a ratio of 105 spermatozoa/egg. Both sequential stripping procedures yielded larger volumes of viable spermatozoa than did a single collection. Sperm parameters such as density and volume varied widely depending on collection time. The highest numbers of spermatozoa per individual were collected 15-42 h post-CPE treatment in (A) and at 42 h post-CPE treatment in (B) (85+/-4% and 64+/-5% of total spermatozoa count, respectively). Median percent motility in spermatozoa before cryopreservation was 26-100% and 5-67% post-thaw. Fertilization rates obtained with frozen/thawed spermatozoa were 13-76% (median value). A significant increase in spermatozoa motility parameters and fertilizing ability at the second collection on each day was observed. Sequential stripping and spermatozoa cryopreservation in combination could improve the efficiency of sturgeon aquaculture.
dcterms:title
Spermatozoa motility, cryoresistance, and fertilizing ability in sterlet Acipenser ruthenus during sequential stripping Spermatozoa motility, cryoresistance, and fertilizing ability in sterlet Acipenser ruthenus during sequential stripping
skos:prefLabel
Spermatozoa motility, cryoresistance, and fertilizing ability in sterlet Acipenser ruthenus during sequential stripping Spermatozoa motility, cryoresistance, and fertilizing ability in sterlet Acipenser ruthenus during sequential stripping
skos:notation
RIV/60076658:12520/12:43883408!RIV13-MZE-12520___
n15:predkladatel
n16:orjk%3A12520
n3:aktivita
n4:S n4:P
n3:aktivity
P(ED2.1.00/01.0024), P(GAP502/11/0090), P(IAA608030801), P(LC06073), P(ME10015), P(QH82119), S
n3:cisloPeriodika
08
n3:dodaniDat
n17:2013
n3:domaciTvurceVysledku
Boryshpolets, Sergey n12:3624900 Dzyuba, Viktoria Shaliutina, Anna Dzyuba, Boris n12:5876001 n12:1593579
n3:druhVysledku
n9:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
170417
n3:idVysledku
RIV/60076658:12520/12:43883408
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Cryopreservation; Motility; Sperm production; Sturgeon
n3:klicoveSlovo
n5:Motility n5:Cryopreservation n5:Sturgeon n5:Sperm%20production
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[687323E46DA8]
n3:nazevZdroje
Aquaculture
n3:obor
n14:GL
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
8
n3:projekt
n6:IAA608030801 n6:ME10015 n6:GAP502%2F11%2F0090 n6:QH82119 n6:LC06073 n6:ED2.1.00%2F01.0024
n3:rokUplatneniVysledku
n17:2012
n3:svazekPeriodika
356
n3:tvurceVysledku
Gela, David Rodina, Marek Yamaner, G. Shaliutina, Anna Dzyuba, Viktoria Linhart, Otomar Dzyuba, Boris Boryshpolets, Sergey
n3:wos
000306171100036
s:issn
0044-8486
s:numberOfPages
7
n8:doi
10.1016/j.aquaculture.2012.05.006
n20:organizacniJednotka
12520