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Statements

Subject Item
n2:RIV%2F60076658%3A12520%2F11%3A43870962%21RIV12-MSM-12520___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
In present study, the complete GH gene was cloned from 17 tench individuals with various genetic distances and sequenced. The tench GH gene was found to be 1758-1763 bp in length. Altogether 14 polymorphic sites were observed, 12 located in introns (SNPs and very short indels) and two in exons (SNPs only). In non-coding regions, the highest polymorphism was found in intron 2 (five SNPs and two indels). In coding regions, one polymorphic site was observed in exon 3 and one in exon 5. The detected SNPs in exons were synonymous. Thirteen GH gene haplotypes were observed. The tench GH gene haplotypes clustered into two major classes with a mean genetic distance of 0.0037+/-0.0014 and coefficient of GH gene differentiation of 0.67+/-0.129 (mean+/-S.E.). Described tench GH gene haplotypes might not include all the existing ones, but all common polymorphic sites were identified. It seems that GH gene polymorphisms in tench can serve as markers for population studies and conservation and breeding purposes. In present study, the complete GH gene was cloned from 17 tench individuals with various genetic distances and sequenced. The tench GH gene was found to be 1758-1763 bp in length. Altogether 14 polymorphic sites were observed, 12 located in introns (SNPs and very short indels) and two in exons (SNPs only). In non-coding regions, the highest polymorphism was found in intron 2 (five SNPs and two indels). In coding regions, one polymorphic site was observed in exon 3 and one in exon 5. The detected SNPs in exons were synonymous. Thirteen GH gene haplotypes were observed. The tench GH gene haplotypes clustered into two major classes with a mean genetic distance of 0.0037+/-0.0014 and coefficient of GH gene differentiation of 0.67+/-0.129 (mean+/-S.E.). Described tench GH gene haplotypes might not include all the existing ones, but all common polymorphic sites were identified. It seems that GH gene polymorphisms in tench can serve as markers for population studies and conservation and breeding purposes.
dcterms:title
Growth hormone gene polymorphisms in tench, Tinca tinca L. Growth hormone gene polymorphisms in tench, Tinca tinca L.
skos:prefLabel
Growth hormone gene polymorphisms in tench, Tinca tinca L. Growth hormone gene polymorphisms in tench, Tinca tinca L.
skos:notation
RIV/60076658:12520/11:43870962!RIV12-MSM-12520___
n16:predkladatel
n17:orjk%3A12520
n3:aktivita
n9:S n9:P
n3:aktivity
P(ED2.1.00/01.0024), S
n3:cisloPeriodika
3-4
n3:dodaniDat
n20:2012
n3:domaciTvurceVysledku
n18:1696211
n3:druhVysledku
n12:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
201582
n3:idVysledku
RIV/60076658:12520/11:43870962
n3:jazykVysledku
n5:eng
n3:klicovaSlova
Tinca tinca; Tench; Sequencing; Polymorphism; Haplotype; Growth hormone; Cloning
n3:klicoveSlovo
n4:Cloning n4:Tench n4:Tinca%20tinca n4:Sequencing n4:Growth%20hormone n4:Polymorphism n4:Haplotype
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[FB7D496BBFBB]
n3:nazevZdroje
Aquaculture
n3:obor
n13:GL
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
2
n3:projekt
n14:ED2.1.00%2F01.0024
n3:rokUplatneniVysledku
n20:2011
n3:svazekPeriodika
310
n3:tvurceVysledku
Kocour, Martin Kohlmann, K.
n3:wos
000286548300008
s:issn
0044-8486
s:numberOfPages
7
n7:doi
10.1016/j.aquaculture.2010.10.006
n11:organizacniJednotka
12520