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Statements

Subject Item
n2:RIV%2F60076658%3A12310%2F12%3A43884040%21RIV13-MSM-12310___
rdf:type
n7:Vysledek skos:Concept
dcterms:description
We present time alignment based on blank measurement as analytical approach for reduction of time shift in high performance liquid chromatography - mass spectrometry (HPLC-MS). Our approach is based on the measurement and comparison of the distinct features of the blank and the analyzed sample. Blank measurement based time alignment (BBTA) utilizes solvent contaminants and other important events detectable both in blank run and the compared experiment for alignment of multiple 2D chromatograms. Several metrics for comparison of mass spectra were evaluated. Marker selection works efficiently even when no peaks are detected. It is capable of analyzing the baseline contribution in analyte measurement when the knowledge of blank behavior is limited. Finally, BBTA because of its simplicity has the potential to be widely used in HPLC-MS data treatment. We present time alignment based on blank measurement as analytical approach for reduction of time shift in high performance liquid chromatography - mass spectrometry (HPLC-MS). Our approach is based on the measurement and comparison of the distinct features of the blank and the analyzed sample. Blank measurement based time alignment (BBTA) utilizes solvent contaminants and other important events detectable both in blank run and the compared experiment for alignment of multiple 2D chromatograms. Several metrics for comparison of mass spectra were evaluated. Marker selection works efficiently even when no peaks are detected. It is capable of analyzing the baseline contribution in analyte measurement when the knowledge of blank behavior is limited. Finally, BBTA because of its simplicity has the potential to be widely used in HPLC-MS data treatment.
dcterms:title
Time-alignment in high performance liquid chromatography - mass spectrometry based on blank measurement Time-alignment in high performance liquid chromatography - mass spectrometry based on blank measurement
skos:prefLabel
Time-alignment in high performance liquid chromatography - mass spectrometry based on blank measurement Time-alignment in high performance liquid chromatography - mass spectrometry based on blank measurement
skos:notation
RIV/60076658:12310/12:43884040!RIV13-MSM-12310___
n7:predkladatel
n22:orjk%3A12310
n3:aktivita
n17:P n17:Z
n3:aktivity
P(ED2.1.00/01.0024), Z(MSM6007665808)
n3:dodaniDat
n6:2013
n3:domaciTvurceVysledku
n5:5093279
n3:druhVysledku
n11:B
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
174468
n3:idVysledku
RIV/60076658:12310/12:43884040
n3:jazykVysledku
n12:eng
n3:klicovaSlova
measurement; blank; based; spectrometry; mass; chromatography; liquid; performance; high; Time-alignment
n3:klicoveSlovo
n4:liquid n4:Time-alignment n4:based n4:mass n4:measurement n4:chromatography n4:spectrometry n4:high n4:blank n4:performance
n3:kontrolniKodProRIV
[E9009D374212]
n3:mistoVydani
Saarbrücken
n3:nazevEdiceCisloSvazku
Neuveden
n3:nazevZdroje
Time-alignment in high performance liquid chromatography - mass spectrometry based on blank measurement
n3:obor
n18:CE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetStranKnihy
108
n3:pocetTvurcuVysledku
3
n3:projekt
n20:ED2.1.00%2F01.0024
n3:rokUplatneniVysledku
n6:2012
n3:tvurceVysledku
Urban, Jan Štys, Dalibor Hrouzek, Pavel
n3:zamer
n13:MSM6007665808
s:numberOfPages
108
n15:hasPublisher
LAMBERT Academic Publishing GmBH & Co.
n16:isbn
978-3-659-13020-5
n21:organizacniJednotka
12310