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Statements

Subject Item
n2:RIV%2F60076658%3A12310%2F12%3A43883570%21RIV13-MSM-12310___
rdf:type
n11:Vysledek skos:Concept
dcterms:description
Wolbachia is a genus of bacterial endosymbionts that impacts the breeding systems of their hosts. Wolbachia can confuse the patterns of mitochondrial variation, including DNA barcodes, because it influences the pathways through which mitochondria are inherited. We examined the extent to which these endosymbionts are detected in routine DNA barcoding, assessed their impact upon the insect sequence divergence and identification accuracy, and considered the variation present in Wolbachia COI. Using both standard PCR assays (Wolbachia surface coding protein - wsp), and bacterial COI fragments we found evidence of Wolbachia in insect total genomic extracts created for DNA barcoding library construction. When }2 million insect COI trace files were examined on the Barcode of Life Datasystem (BOLD) Wolbachia COI was present in 0.16% of the cases. It is possible to generate Wolbachia COI using standard insect primers; however, that amplicon was never confused with the COI of the host. Wolbachia alleles recovered were predominantly Supergroup A and were broadly distributed geographically and phylogenetically. We conclude that the presence of the Wolbachia DNA in total genomic extracts made from insects is unlikely to compromise the accuracy of the DNA barcode library; in fact, the ability to query this DNA library (the database and the extracts) for endosymbionts is one of the ancillary benefits of such a large scale endeavor - for which we provide several examples. It is our conclusion that regular assays for Wolbachia presence and type can, and should, be adopted by large scale insect barcoding initiatives. While COI is one of the five multi-locus sequence typing (MLST) genes used for categorizing Wolbachia, there is limited overlap with the eukaryotic DNA barcode region. Wolbachia is a genus of bacterial endosymbionts that impacts the breeding systems of their hosts. Wolbachia can confuse the patterns of mitochondrial variation, including DNA barcodes, because it influences the pathways through which mitochondria are inherited. We examined the extent to which these endosymbionts are detected in routine DNA barcoding, assessed their impact upon the insect sequence divergence and identification accuracy, and considered the variation present in Wolbachia COI. Using both standard PCR assays (Wolbachia surface coding protein - wsp), and bacterial COI fragments we found evidence of Wolbachia in insect total genomic extracts created for DNA barcoding library construction. When }2 million insect COI trace files were examined on the Barcode of Life Datasystem (BOLD) Wolbachia COI was present in 0.16% of the cases. It is possible to generate Wolbachia COI using standard insect primers; however, that amplicon was never confused with the COI of the host. Wolbachia alleles recovered were predominantly Supergroup A and were broadly distributed geographically and phylogenetically. We conclude that the presence of the Wolbachia DNA in total genomic extracts made from insects is unlikely to compromise the accuracy of the DNA barcode library; in fact, the ability to query this DNA library (the database and the extracts) for endosymbionts is one of the ancillary benefits of such a large scale endeavor - for which we provide several examples. It is our conclusion that regular assays for Wolbachia presence and type can, and should, be adopted by large scale insect barcoding initiatives. While COI is one of the five multi-locus sequence typing (MLST) genes used for categorizing Wolbachia, there is limited overlap with the eukaryotic DNA barcode region.
dcterms:title
Wolbachia and DNA Barcoding Insects: Patterns, Potential, and Problems Wolbachia and DNA Barcoding Insects: Patterns, Potential, and Problems
skos:prefLabel
Wolbachia and DNA Barcoding Insects: Patterns, Potential, and Problems Wolbachia and DNA Barcoding Insects: Patterns, Potential, and Problems
skos:notation
RIV/60076658:12310/12:43883570!RIV13-MSM-12310___
n11:predkladatel
n17:orjk%3A12310
n3:aktivita
n5:P n5:I n5:V n5:Z
n3:aktivity
I, P(GAP505/10/0673), V, Z(AV0Z50070508)
n3:cisloPeriodika
5
n3:dodaniDat
n18:2013
n3:domaciTvurceVysledku
n21:6057624
n3:druhVysledku
n10:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
181034
n3:idVysledku
RIV/60076658:12310/12:43883570
n3:jazykVysledku
n20:eng
n3:klicovaSlova
IDENTIFICATIONS; ANTS; GENUS; BIODIVERSITY; DIVERSITY; SELECTIVE SWEEPS; BACTERIAL SYMBIONTS; HYBRID INTROGRESSION; MITOCHONDRIAL-DNA; PARASITOID FLIES DIPTERA
n3:klicoveSlovo
n9:DIVERSITY n9:HYBRID%20INTROGRESSION n9:BACTERIAL%20SYMBIONTS n9:ANTS n9:SELECTIVE%20SWEEPS n9:IDENTIFICATIONS n9:GENUS n9:PARASITOID%20FLIES%20DIPTERA n9:BIODIVERSITY n9:MITOCHONDRIAL-DNA
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[8C3593525F86]
n3:nazevZdroje
PLOS ONE
n3:obor
n8:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
28
n3:projekt
n19:GAP505%2F10%2F0673
n3:rokUplatneniVysledku
n18:2012
n3:svazekPeriodika
7
n3:tvurceVysledku
Quicke, Donald Fernandez-Triana, Jose Crosby, Kate Ratnasingham, Sujeevan Gibbs, Jason Li, Yanwei Janzen, Daniel H. Whitfield, James Stahlhut, Julie K. Sheffield, Cory Janda, Milan Hrček, Jan Shaw, Mark R. Huang, Da-Wei Smith, M. Alex Packer, Laurence Bertrand, Claudia Wood, Monty Steinke, Dirk Fisher, Brian L. Rougerie, Rodolphe Miller, Scott E. Hajibabaei, Mehrdad Hallwachs, Winnie Rodriguez, Josephine Hind, Katharine Eveleigh, Eldon S. Zhou, Xin
n3:wos
000305341500082
n3:zamer
n13:AV0Z50070508
s:issn
1932-6203
s:numberOfPages
12
n15:doi
10.1371/journal.pone.0036514
n4:organizacniJednotka
12310