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Statements

Subject Item
n2:RIV%2F60076658%3A12310%2F03%3A00008614%21RIV08-MSM-12310___
rdf:type
n11:Vysledek skos:Concept
dcterms:description
The transcription factor Broad-Complex (BR-C) is required for differentiation of adult structures as well as for the programmed death of obsolete larval organs during metamorphosis of the fruit fly Drosophila melanogaster. Whether BR-C has a similar role in other holometabolous insects could not be proven without a loss-of-function genetic test, performed in a non-drosophilid species. Here we use a recombinant Sindbis virus as a tool to silence BR-C expression in the silkmoth Bombyx mori. The virus expressing a BR-C antisense RNA fragment reduced endogenous BR-C mRNA levels in infected tissues (adult wing and leg primordia) via RNA interference (RNAi). The RNAi knock-down of BR-C resulted in the failure of animals to complete the larval-pupal transition or in later morphogenetic defects, including differentiation of adult compound eyes, legs, and wings from their larval progenitors. BR-C RNAi also perturbed the programmed cell death of larval silk glands. These developmental defects correspond to loss The transcription factor Broad-Complex (BR-C) is required for differentiation of adult structures as well as for the programmed death of obsolete larval organs during metamorphosis of the fruit fly Drosophila melanogaster. Whether BR-C has a similar role in other holometabolous insects could not be proven without a loss-of-function genetic test, performed in a non-drosophilid species. Here we use a recombinant Sindbis virus as a tool to silence BR-C expression in the silkmoth Bombyx mori. The virus expressing a BR-C antisense RNA fragment reduced endogenous BR-C mRNA levels in infected tissues (adult wing and leg primordia) via RNA interference (RNAi). The RNAi knock-down of BR-C resulted in the failure of animals to complete the larval-pupal transition or in later morphogenetic defects, including differentiation of adult compound eyes, legs, and wings from their larval progenitors. BR-C RNAi also perturbed the programmed cell death of larval silk glands. These developmental defects correspond to loss Pouziti Sindbis virem navozene RNA interference k ukazani role Broad komplexu v hmyzi promene.
dcterms:title
Use of Sindbis virus-mediated RNA interference to demonstrate a conserved role of Broad-Complex in insect metamorphosis. Use of Sindbis virus-mediated RNA interference to demonstrate a conserved role of Broad-Complex in insect metamorphosis. Pouziti Sindbis virem navozene RNA interference k ukazani role Broad komplexu v hmyzi promene.
skos:prefLabel
Use of Sindbis virus-mediated RNA interference to demonstrate a conserved role of Broad-Complex in insect metamorphosis. Use of Sindbis virus-mediated RNA interference to demonstrate a conserved role of Broad-Complex in insect metamorphosis. Pouziti Sindbis virem navozene RNA interference k ukazani role Broad komplexu v hmyzi promene.
skos:notation
RIV/60076658:12310/03:00008614!RIV08-MSM-12310___
n3:strany
15607-15612
n3:aktivita
n8:P n8:V n8:Z
n3:aktivity
P(IAA5007305), V, Z(AV0Z5007907)
n3:cisloPeriodika
26
n3:dodaniDat
n15:2008
n3:domaciTvurceVysledku
n14:9460551
n3:druhVysledku
n9:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
632340
n3:idVysledku
RIV/60076658:12310/03:00008614
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Drosophila metamorphosis; manducasexta; induced expression; juvenile-hormone; tobacco hornworm; aedes-aegypti
n3:klicoveSlovo
n10:aedes-aegypti n10:tobacco%20hornworm n10:induced%20expression n10:manducasexta n10:juvenile-hormone n10:Drosophila%20metamorphosis
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[4155975CA8D1]
n3:nazevZdroje
Proceedings of The National Academy of Sciences of The United States of America
n3:obor
n6:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
6
n3:projekt
n13:IAA5007305
n3:rokUplatneniVysledku
n15:2003
n3:svazekPeriodika
100
n3:tvurceVysledku
Olson, K. E. Riddiford, L. M. Foy, D. B. Uhlirova, M. Jindra, Marek Beaty, B. J.
n3:zamer
n17:AV0Z5007907
s:issn
0027-8424
s:numberOfPages
6
n16:organizacniJednotka
12310