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Statements

Subject Item
n2:RIV%2F60076658%3A12220%2F15%3A43887835%21RIV15-GA0-12220___
rdf:type
n18:Vysledek skos:Concept
dcterms:description
We tested 212 bacterial isolates isolated from tomato and pepper plants suspected of being infected by Xanthomonas vesicatoria (causing bacterial spot). Samples from plants were collected in the years 2007- 2010 from fields located in central Bohemia and central and south Moravia. For detection of the pathogens we used PCR with two previously published primer pairs (RST65, RST69 and XCVF, XCVR) and one primer pair (Xv1, Xv2) designed for specific detection of Xanthomonas vesicatoria. In total, 121 of 212 (57%) samples were positive in PCR. In this article we provide data for additional verification of the newly designed (Xv1, Xv2) primers. Our results confirm the need of precise detection methods, because several tomato and pepper pathogens can share similar visual symptoms. We also suggest, that new PCR primers for detection of bacterial spot of tomato and pepper pathogens should not be overlooked, because they can improve currently used PCR assays for detection of these pathogens. We tested 212 bacterial isolates isolated from tomato and pepper plants suspected of being infected by Xanthomonas vesicatoria (causing bacterial spot). Samples from plants were collected in the years 2007- 2010 from fields located in central Bohemia and central and south Moravia. For detection of the pathogens we used PCR with two previously published primer pairs (RST65, RST69 and XCVF, XCVR) and one primer pair (Xv1, Xv2) designed for specific detection of Xanthomonas vesicatoria. In total, 121 of 212 (57%) samples were positive in PCR. In this article we provide data for additional verification of the newly designed (Xv1, Xv2) primers. Our results confirm the need of precise detection methods, because several tomato and pepper pathogens can share similar visual symptoms. We also suggest, that new PCR primers for detection of bacterial spot of tomato and pepper pathogens should not be overlooked, because they can improve currently used PCR assays for detection of these pathogens.
dcterms:title
Monitoring the occurrence of bacterial spot of tomato and pepper in the Czech Republic and development of new PCR primers for detection of Xanthomonas vesicatoria Monitoring the occurrence of bacterial spot of tomato and pepper in the Czech Republic and development of new PCR primers for detection of Xanthomonas vesicatoria
skos:prefLabel
Monitoring the occurrence of bacterial spot of tomato and pepper in the Czech Republic and development of new PCR primers for detection of Xanthomonas vesicatoria Monitoring the occurrence of bacterial spot of tomato and pepper in the Czech Republic and development of new PCR primers for detection of Xanthomonas vesicatoria
skos:notation
RIV/60076658:12220/15:43887835!RIV15-GA0-12220___
n3:aktivita
n15:P
n3:aktivity
P(EE2.3.30.0049), P(GD521/08/H042)
n3:cisloPeriodika
141
n3:dodaniDat
n9:2015
n3:domaciTvurceVysledku
n13:8594694 n13:5529182 n13:1658670
n3:druhVysledku
n16:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
287
n3:idVysledku
RIV/60076658:12220/15:43887835
n3:jazykVysledku
n17:eng
n3:klicovaSlova
atpD,Oligonucleotides, Xanthomonas euvesicatoria, Lycopersicon esculentum, Capsicum annuum
n3:klicoveSlovo
n7:Xanthomonas%20euvesicatoria n7:Oligonucleotides n7:Lycopersicon%20esculentum n7:Capsicum%20annuum n7:atpD
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[47601AA08D9F]
n3:nazevZdroje
European Journal of Plant Pathology
n3:obor
n4:EE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:projekt
n8:EE2.3.30.0049 n8:GD521%2F08%2FH042
n3:rokUplatneniVysledku
n9:2015
n3:svazekPeriodika
2015
n3:tvurceVysledku
Beran, Pavel Kokošková, Blanka Mráz, Ivan Bohatá, Andrea
s:issn
0929-1873
s:numberOfPages
5
n10:doi
10.1007/s10658-014-0554-y
n19:organizacniJednotka
12220