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Statements

Subject Item
n2:RIV%2F60076658%3A12220%2F14%3A43887792%21RIV15-MZE-12220___
rdf:type
n10:Vysledek skos:Concept
dcterms:description
The selection of desirable genotypes with recessive characteristics, such as self-incompatible plants, is often difficult or even impossible and represents a crucial barrier in accelerating the breeding process. Molecular approaches and selection based on molecular markers can allow breeders to overcome this limitation. The use of self-incompatibility is an alternative in hybrid breeding of oilseed rape. Unfortunately, stable self-incompatibility is recessive and phenotype-based selection is very difficult and time-consuming. The development of reliable molecular markers for detecting desirable plants with functional self-incompatible genes is of great importance for breeders and allows selection at early stages of plant growth. Because most of these reliable molecular markers are based on discrimination of class I S-locus genes that are present in self-compatible plants, there is a need to use an internal control in order to detect possible PCR inhibition that gives false results during genotyping. In this study, 269 double haploid F 2 oilseed rape plants obtained by microspore embryogenesis were used to verify the applicability of an improved PCR assay based on the detection of the class ISLG gene along with an internal control. Comparative analysis of the PCR genotyping results vs. S phenotype analysis confirmed the applicability of this molecular approach in hybrid breeding programs. This approach allows accurate detection of self-incompatible plants via a different amplification profile. The selection of desirable genotypes with recessive characteristics, such as self-incompatible plants, is often difficult or even impossible and represents a crucial barrier in accelerating the breeding process. Molecular approaches and selection based on molecular markers can allow breeders to overcome this limitation. The use of self-incompatibility is an alternative in hybrid breeding of oilseed rape. Unfortunately, stable self-incompatibility is recessive and phenotype-based selection is very difficult and time-consuming. The development of reliable molecular markers for detecting desirable plants with functional self-incompatible genes is of great importance for breeders and allows selection at early stages of plant growth. Because most of these reliable molecular markers are based on discrimination of class I S-locus genes that are present in self-compatible plants, there is a need to use an internal control in order to detect possible PCR inhibition that gives false results during genotyping. In this study, 269 double haploid F 2 oilseed rape plants obtained by microspore embryogenesis were used to verify the applicability of an improved PCR assay based on the detection of the class ISLG gene along with an internal control. Comparative analysis of the PCR genotyping results vs. S phenotype analysis confirmed the applicability of this molecular approach in hybrid breeding programs. This approach allows accurate detection of self-incompatible plants via a different amplification profile.
dcterms:title
Detection of self-incompatible oilseed rape plants (Brassica napus L.) based on molecular markers for identification of the class I S haplotype Detection of self-incompatible oilseed rape plants (Brassica napus L.) based on molecular markers for identification of the class I S haplotype
skos:prefLabel
Detection of self-incompatible oilseed rape plants (Brassica napus L.) based on molecular markers for identification of the class I S haplotype Detection of self-incompatible oilseed rape plants (Brassica napus L.) based on molecular markers for identification of the class I S haplotype
skos:notation
RIV/60076658:12220/14:43887792!RIV15-MZE-12220___
n3:aktivita
n6:P n6:S
n3:aktivity
P(EE2.3.30.0006), P(QI111A075), S
n3:cisloPeriodika
37
n3:dodaniDat
n9:2015
n3:domaciTvurceVysledku
n4:9375201 n4:1739859
n3:druhVysledku
n18:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
10635
n3:idVysledku
RIV/60076658:12220/14:43887792
n3:jazykVysledku
n17:eng
n3:klicovaSlova
SLG; S locus; MAS; hybrid breeding; double haploid
n3:klicoveSlovo
n8:hybrid%20breeding n8:double%20haploid n8:MAS n8:SLG n8:S%20locus
n3:kodStatuVydavatele
BR - Brazilská federativní republika
n3:kontrolniKodProRIV
[FF741A7F7567]
n3:nazevZdroje
Genetics and Molecular Biology
n3:obor
n16:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
5
n3:projekt
n14:EE2.3.30.0006 n14:QI111A075
n3:rokUplatneniVysledku
n9:2014
n3:svazekPeriodika
2014
n3:tvurceVysledku
Kučera, Vratislav Klíma, Miroslav Havlíčková, Lenka Čurn, Vladislav Jozová, Eva
s:issn
1415-4757
s:numberOfPages
4
n13:organizacniJednotka
12220