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Statements

Subject Item
n2:RIV%2F60076658%3A12220%2F13%3A43886150%21RIV14-MZE-12220___
rdf:type
n11:Vysledek skos:Concept
dcterms:description
There are several approaches available for hybrid breeding in oil seed rape, Brassica napus, as cytoplasmic male sterility (CMS), genic male sterility (GMS), self-incompatibility (SI), and chemical hybridizing agens (CHA). In comparison with others, SI is regarded as one of the most valuable strategies in hybrid breeding. Unlike self-incompatible (SI) B. rapa and B. oleracea, two ancestor species, B. napus is naturally self-compatible (SC). However, occasionally SI also occurs in rapeseed cultivars. SI in Brassicaceae plants is sporophytically controlled by a single multi-allelic locus (S-locus), which contains at least three highly polymorphic genes expressed in the stigma (S locus glycoprotein, SLG and S receptor kinase, SRK) and in the pollen (SCR/SP11). In segregating population derived from crosses between DH SI lines and 00-quality donors we found two recessive alleles of a SCR class II gene. We developed new primers for detection of unique cv. Tandem derived allele and this allele was successfully amplified in SI donor plants and SI plants after first cycle of crossing. Analyses of other accessions (SI donor different from cv. Tandem) and varieties did not show so clear pattern of segregation and different expression of both alleles does not correspond to phenotypic manifestation of self-incompatibility and we can assume that it is caused by the presence of repressor gene that does not lie on the S-locus. There are several approaches available for hybrid breeding in oil seed rape, Brassica napus, as cytoplasmic male sterility (CMS), genic male sterility (GMS), self-incompatibility (SI), and chemical hybridizing agens (CHA). In comparison with others, SI is regarded as one of the most valuable strategies in hybrid breeding. Unlike self-incompatible (SI) B. rapa and B. oleracea, two ancestor species, B. napus is naturally self-compatible (SC). However, occasionally SI also occurs in rapeseed cultivars. SI in Brassicaceae plants is sporophytically controlled by a single multi-allelic locus (S-locus), which contains at least three highly polymorphic genes expressed in the stigma (S locus glycoprotein, SLG and S receptor kinase, SRK) and in the pollen (SCR/SP11). In segregating population derived from crosses between DH SI lines and 00-quality donors we found two recessive alleles of a SCR class II gene. We developed new primers for detection of unique cv. Tandem derived allele and this allele was successfully amplified in SI donor plants and SI plants after first cycle of crossing. Analyses of other accessions (SI donor different from cv. Tandem) and varieties did not show so clear pattern of segregation and different expression of both alleles does not correspond to phenotypic manifestation of self-incompatibility and we can assume that it is caused by the presence of repressor gene that does not lie on the S-locus.
dcterms:title
Different expression of S-locus cysteine-rich protein (SCR) alleles in self-incompatible and self-compatible Brassica napus breeding lines and cultivars: Can be SCR/SP11 used as a selectable marker in breeding? Different expression of S-locus cysteine-rich protein (SCR) alleles in self-incompatible and self-compatible Brassica napus breeding lines and cultivars: Can be SCR/SP11 used as a selectable marker in breeding?
skos:prefLabel
Different expression of S-locus cysteine-rich protein (SCR) alleles in self-incompatible and self-compatible Brassica napus breeding lines and cultivars: Can be SCR/SP11 used as a selectable marker in breeding? Different expression of S-locus cysteine-rich protein (SCR) alleles in self-incompatible and self-compatible Brassica napus breeding lines and cultivars: Can be SCR/SP11 used as a selectable marker in breeding?
skos:notation
RIV/60076658:12220/13:43886150!RIV14-MZE-12220___
n11:predkladatel
n12:orjk%3A12220
n4:aktivita
n10:S n10:P
n4:aktivity
P(EE2.3.30.0006), P(QI111A075), S
n4:cisloPeriodika
8
n4:dodaniDat
n5:2014
n4:domaciTvurceVysledku
n9:1739859 n9:9375201 n9:4494091
n4:druhVysledku
n6:J
n4:duvernostUdaju
n20:S
n4:entitaPredkladatele
n7:predkladatel
n4:idSjednocenehoVysledku
69624
n4:idVysledku
RIV/60076658:12220/13:43886150
n4:jazykVysledku
n16:eng
n4:klicovaSlova
-incompatibility, Brassica napus, S receptor kinase (SRK), S locus glycoprotein (SLG), S locus cysteine-rich protein (SCR).
n4:klicoveSlovo
n15:Brassica%20napus n15:S%20locus%20cysteine-rich%20protein%20%28SCR%29. n15:S%20locus%20glycoprotein%20%28SLG%29 n15:S%20receptor%20kinase%20%28SRK%29 n15:-incompatibility
n4:kodStatuVydavatele
NG - Nigerijská federativní republika
n4:kontrolniKodProRIV
[894D920301ED]
n4:nazevZdroje
African Journal of Agricultural Research
n4:obor
n18:EB
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
4
n4:projekt
n8:EE2.3.30.0006 n8:QI111A075
n4:rokUplatneniVysledku
n5:2013
n4:svazekPeriodika
47
n4:tvurceVysledku
Čurn, Vladislav Žaludová, Jana Havlíčková, Lenka Kukolíková, Božena
s:issn
1991-637X
s:numberOfPages
7
n13:doi
10.5897/AJAR12.969
n17:organizacniJednotka
12220