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Statements

Subject Item
n2:RIV%2F60076658%3A12220%2F10%3A00012481%21RIV11-MSM-12220___
rdf:type
skos:Concept n13:Vysledek
dcterms:description
The simple and reliable method for determination of quercetin glycosides and free quercetin in buckwheat flower, leaves, stems and achenes was developed. The method consists of flavonoid extraction from freeze-dried homogenous material in 50% (v/v) methanol solution and in presence of an antioxidant, cleaning of extract and analyte isolation using solid phase extraction. Analytical step uses capillary micellar electrokinetic chromatography. The working ranges, limit of detection and quantification, recovery, precision and measurement uncertainty were calculated. The method is suitable for samples from buckwheat. The highest content of rutin was found in flowers of both kinds of buckwheat (99 400 mg?kg-1 in F. esculentum, 108 000 99 400 mg?kg-1 in F. tataricum). The free quercetin occurs in flowers and achenes of F. esculentum, while flowers and achenes of F. tataricum contained quercitrin. The simple and reliable method for determination of quercetin glycosides and free quercetin in buckwheat flower, leaves, stems and achenes was developed. The method consists of flavonoid extraction from freeze-dried homogenous material in 50% (v/v) methanol solution and in presence of an antioxidant, cleaning of extract and analyte isolation using solid phase extraction. Analytical step uses capillary micellar electrokinetic chromatography. The working ranges, limit of detection and quantification, recovery, precision and measurement uncertainty were calculated. The method is suitable for samples from buckwheat. The highest content of rutin was found in flowers of both kinds of buckwheat (99 400 mg?kg-1 in F. esculentum, 108 000 99 400 mg?kg-1 in F. tataricum). The free quercetin occurs in flowers and achenes of F. esculentum, while flowers and achenes of F. tataricum contained quercitrin.
dcterms:title
Determination of quercetin glycosides and free quercetin in buckwheat by capillary micellar electrokinetic chromatography Determination of quercetin glycosides and free quercetin in buckwheat by capillary micellar electrokinetic chromatography
skos:prefLabel
Determination of quercetin glycosides and free quercetin in buckwheat by capillary micellar electrokinetic chromatography Determination of quercetin glycosides and free quercetin in buckwheat by capillary micellar electrokinetic chromatography
skos:notation
RIV/60076658:12220/10:00012481!RIV11-MSM-12220___
n3:aktivita
n15:Z
n3:aktivity
Z(MSM6007665806)
n3:cisloPeriodika
11
n3:dodaniDat
n4:2011
n3:domaciTvurceVysledku
n9:4433254 n9:4829417
n3:druhVysledku
n12:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
253798
n3:idVysledku
RIV/60076658:12220/10:00012481
n3:jazykVysledku
n10:eng
n3:klicovaSlova
buckwheat; MEKC; quercetin glycosides
n3:klicoveSlovo
n5:quercetin%20glycosides n5:buckwheat n5:MEKC
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[253166520751]
n3:nazevZdroje
Journal of Separation Science
n3:obor
n18:CB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:rokUplatneniVysledku
n4:2010
n3:svazekPeriodika
33
n3:tvurceVysledku
Kalinová, Jana Dadáková, Eva
n3:wos
000278829700014
n3:zamer
n16:MSM6007665806
s:issn
1615-9306
s:numberOfPages
6
n7:organizacniJednotka
12220