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Statements

Subject Item
n2:RIV%2F60076658%3A12220%2F04%3A00005201%21RIV%2F2005%2FGA0%2F122205%2FN
rdf:type
n9:Vysledek skos:Concept
dcterms:description
The effects of environmental conditions (year, growing site) on protein compounds in buckwheat (Fagopyrum esculentum Moench) were studied. Small-plot experiments with four buckwheat cultivars (tetraploid Emka and diploid Kora, Pyra, and Krupinka) were established in two growing sites in 1999 and 2000. Crude and pure protein content, proportion of protein solubility fractions, SDS-PAGE of protein fractions, and immunoassay of gluten-like proteins were determined in buckwheat flour. The growing site had the most significant effect on variability of crude protein content. On the contrary, pure protein content was most of all affected by year. Apparently a higher occurrence of later developed buckwheat seeds in 1999 harvest (as a consequence of weather abnormalities) could generally cause lower content of pure protein in this year (7.32 %) than in 2000 (9.10 %). Alcohol-soluble protein fraction (prolamins like proteins, PLP), considered as inconvenient for gluten-free diet, was generally found at low leve V příspěvku byly studovány vlivy podmínek prostředí (ročník, stanoviště) na bílkovinné složky pohanky (Fagopyrum esculentum Moench). V letech 1999 a 2000 byly založeny maloparcelkové pokusy se čtyřmi odrůdami pohanky (tetraploidní Emka a diploidní Kora, Pyra a Krupinka na dvou pěstitelských stanovištích. Po sklizni byly v pohankové mouce analyzovány: obsah hrubých a čistých bílkovin, proporce bílkovinných frakcí dle rozpustnosti, bílkovinné frakce pomocí SDS-PAGE a byly provedeny imunochemické testy na obsah gluten-like proteinů. Pěstitelské stanoviště mělo nejvýznamnější vliv na variabilitu obsahu hrubých bílkovin. Naopak obsah čistého proteinu byl většinou ovlivněn ročníkem. Patrně vyšší výskyt později vyvinutých semen při sklizni v roce 1999 (jako následek abnormalit počasí) obecně mohl tvořit nižší obsah čistého proteinu v tomto roce (7.32 %) než v roce 2000 (9.10 %). Frakce bílkovin rozpustných v alkoholu (prolaminu podobné proteiny, PLP), která je považována za nevhodnou pro gluten-free diety, b The effects of environmental conditions (year, growing site) on protein compounds in buckwheat (Fagopyrum esculentum Moench) were studied. Small-plot experiments with four buckwheat cultivars (tetraploid Emka and diploid Kora, Pyra, and Krupinka) were established in two growing sites in 1999 and 2000. Crude and pure protein content, proportion of protein solubility fractions, SDS-PAGE of protein fractions, and immunoassay of gluten-like proteins were determined in buckwheat flour. The growing site had the most significant effect on variability of crude protein content. On the contrary, pure protein content was most of all affected by year. Apparently a higher occurrence of later developed buckwheat seeds in 1999 harvest (as a consequence of weather abnormalities) could generally cause lower content of pure protein in this year (7.32 %) than in 2000 (9.10 %). Alcohol-soluble protein fraction (prolamins like proteins, PLP), considered as inconvenient for gluten-free diet, was generally found at low leve
dcterms:title
Effects of environmental factors on protein content and composition in buckwheat flour Vlivy pěstitelského prostředí na obsah a složení bílkovin v mouce pohanky Effects of environmental factors on protein content and composition in buckwheat flour
skos:prefLabel
Effects of environmental factors on protein content and composition in buckwheat flour Vlivy pěstitelského prostředí na obsah a složení bílkovin v mouce pohanky Effects of environmental factors on protein content and composition in buckwheat flour
skos:notation
RIV/60076658:12220/04:00005201!RIV/2005/GA0/122205/N
n5:strany
541-548
n5:aktivita
n6:Z n6:P
n5:aktivity
P(GA303/01/1380), Z(MSM 122200002)
n5:cisloPeriodika
4
n5:dodaniDat
n10:2005
n5:domaciTvurceVysledku
n12:4375483 n12:1739859 n12:6717187 n12:4433254
n5:druhVysledku
n15:J
n5:duvernostUdaju
n18:S
n5:entitaPredkladatele
n8:predkladatel
n5:idSjednocenehoVysledku
562163
n5:idVysledku
RIV/60076658:12220/04:00005201
n5:jazykVysledku
n11:eng
n5:klicovaSlova
Fagopyrum esculentum Moench;crude protein;pure protein;protein solubility fraction
n5:klicoveSlovo
n7:pure%20protein n7:Fagopyrum%20esculentum%20Moench n7:protein%20solubility%20fraction n7:crude%20protein
n5:kodStatuVydavatele
HU - Maďarsko
n5:kontrolniKodProRIV
[63DB7F5D823F]
n5:nazevZdroje
Cereal Research Communications
n5:obor
n19:GC
n5:pocetDomacichTvurcuVysledku
4
n5:pocetTvurcuVysledku
4
n5:projekt
n14:GA303%2F01%2F1380
n5:rokUplatneniVysledku
n10:2004
n5:svazekPeriodika
32
n5:tvurceVysledku
Kalinová, Jana Čurn, Vladislav Bárta, Jan Moudrý, Jan
n5:zamer
n17:MSM%20122200002
s:issn
0133-3720
s:numberOfPages
8
n16:organizacniJednotka
12220