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Statements

Subject Item
n2:RIV%2F49608851%3A_____%2F13%3A%230000593%21RIV14-MZE-49608851
rdf:type
n9:Vysledek skos:Concept
dcterms:description
This study was aimed at comparison of some sperm characteristics in Czech Fleckvieh (n= 8) and Holstein (n= 8) bulls at the age of 1 to 6 years. After determination of pathologic sperm (staining with Congo red, Bromothymol blue and Janus green) and sperm motility, the samples of semen with progressive movement >=70% were diluted in AndroMed extender containing soybean lecithin extract, frozen in a programmable freezing device and stored in liquid nitrogen until analyses. Sperm motility was analyzed by CASA. Sperm plasma membrane integrity and apoptosis incidence were detected under a Leica fluorescent microscope after staining with PNA-Alexa Fluor and Yo-Pro-I in combination with propidium iodide (PI), using specific wavelength filters. Average age of bulls and incidence of pathological sperm were not different between CF and H breeds, whilst average body condition score (BCS) was significantly higher (p< 0,05) in CF (2.84±0, 17) than in H (2.19±0, 13) bulls. Total sperm motility and progressive movement measured immediately after thawing (38.6±1.8 and 34.19±1.9, resp) and following 0,5 h (46.44±1.79 and 44,14±1.8) were higher in H bulls compared to CF bulls (34,15±2 and 30.27±2,13; 39,17 ±2 and 36.12±2.07, resp.), but following 2 h these traits were not different. More sperm with plasma membrane damages were recorded in CF bulls (36.41±1.81%) compared to H bulls (26.33± 1.63%). Similarly, higher incidence of apoptotic sperm was noted in CF bulls (19.12±1.71%) compared to H bulls (12.58± 1.51 %). We observed differences in sperm motility and viability between two tested breeds, which may be a result of interbreed difference in average body condition. Makarevič., A. – Špaleková, E. – Stádník, L. – Kubovičová, E. – Louda, F.: Breed differences in several characteristics of bull frozen-thawed sperm. 10th Int. Sci. Conference Animal Physiology 2013 (June 4th-6th 2013; Crocus-Kežmarské Žľaby). In: J. Microbiol. Biotechnol. Food Sci., 2013:2 (Special Issue), s. 6. This study was aimed at comparison of some sperm characteristics in Czech Fleckvieh (n= 8) and Holstein (n= 8) bulls at the age of 1 to 6 years. After determination of pathologic sperm (staining with Congo red, Bromothymol blue and Janus green) and sperm motility, the samples of semen with progressive movement >=70% were diluted in AndroMed extender containing soybean lecithin extract, frozen in a programmable freezing device and stored in liquid nitrogen until analyses. Sperm motility was analyzed by CASA. Sperm plasma membrane integrity and apoptosis incidence were detected under a Leica fluorescent microscope after staining with PNA-Alexa Fluor and Yo-Pro-I in combination with propidium iodide (PI), using specific wavelength filters. Average age of bulls and incidence of pathological sperm were not different between CF and H breeds, whilst average body condition score (BCS) was significantly higher (p< 0,05) in CF (2.84±0, 17) than in H (2.19±0, 13) bulls. Total sperm motility and progressive movement measured immediately after thawing (38.6±1.8 and 34.19±1.9, resp) and following 0,5 h (46.44±1.79 and 44,14±1.8) were higher in H bulls compared to CF bulls (34,15±2 and 30.27±2,13; 39,17 ±2 and 36.12±2.07, resp.), but following 2 h these traits were not different. More sperm with plasma membrane damages were recorded in CF bulls (36.41±1.81%) compared to H bulls (26.33± 1.63%). Similarly, higher incidence of apoptotic sperm was noted in CF bulls (19.12±1.71%) compared to H bulls (12.58± 1.51 %). We observed differences in sperm motility and viability between two tested breeds, which may be a result of interbreed difference in average body condition. Makarevič., A. – Špaleková, E. – Stádník, L. – Kubovičová, E. – Louda, F.: Breed differences in several characteristics of bull frozen-thawed sperm. 10th Int. Sci. Conference Animal Physiology 2013 (June 4th-6th 2013; Crocus-Kežmarské Žľaby). In: J. Microbiol. Biotechnol. Food Sci., 2013:2 (Special Issue), s. 6.
dcterms:title
Breed differences in several characteristics of bull frozen-thawed sperm Breed differences in several characteristics of bull frozen-thawed sperm
skos:prefLabel
Breed differences in several characteristics of bull frozen-thawed sperm Breed differences in several characteristics of bull frozen-thawed sperm
skos:notation
RIV/49608851:_____/13:#0000593!RIV14-MZE-49608851
n9:predkladatel
n11:ico%3A49608851
n3:aktivita
n8:P
n3:aktivity
P(QI91A061)
n3:dodaniDat
n16:2014
n3:domaciTvurceVysledku
n4:7976089
n3:druhVysledku
n10:O
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
63852
n3:idVysledku
RIV/49608851:_____/13:#0000593
n3:jazykVysledku
n12:eng
n3:klicovaSlova
Holstein; Czech Fleckvieh; bull; sperm; motility; plasma membrane; apoptosis
n3:klicoveSlovo
n6:bull n6:Holstein n6:apoptosis n6:plasma%20membrane n6:Czech%20Fleckvieh n6:motility n6:sperm
n3:kontrolniKodProRIV
[ADDC9E2B4FF4]
n3:obor
n15:GI
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:projekt
n14:QI91A061
n3:rokUplatneniVysledku
n16:2013
n3:tvurceVysledku
Stádník, Luděk Špaleková, Eliška Kubovičová, Elena Louda, František Makarevič, Alexander