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Statements

Subject Item
n2:RIV%2F49608851%3A_____%2F12%3A%230000581%21RIV13-MZE-49608851
rdf:type
skos:Concept n8:Vysledek
rdfs:seeAlso
http://dx.doi.org/10.1017/S0967199412000500
dcterms:description
The study was aimed at examining the effects of IGF-I on ram sperm traits after hypothermic (4ºC) storage. Sperm ejaculates from 3 Lacaune rams were diluted in Triladyl, pooled and divided into the groups of IGF-I doses added (0, 10, 100 or 200 ng/ml). Following 72 h of storage the sperm samples were stained for a plasma membrane integrity (peanut agglutinin, PNA-FITC), membrane stability (annexin V-FITC) and apoptosis (Yo-Pro-1) and analyzed under a fluorescent microscope. Sperm motility was determined by CASA (at 0, 24 and 48h) and fertilizing ability (at 48h) - by an in vitro fertilization (IVF) test on bovine oocytes. IGF-I (100 and 200 ng/ml) decreased the portion of spermatozoa with disrupted plasma membrane integrity (15.9 and 16.5% resp. vs 28.3% in control, p< 0.05) and, given at all tested doses, reduced the rate of apoptotic sperm (6.2, 4.9 and 4.4% resp. vs 9.7% in control, p< 0.05). The proportion of spermatozoa with annexin V-labelled membrane changes was reduced by IGF-I from 9.6% (control) to 4.9% (100 ng/ml) and 6.1% (200 ng/ml). IGF-I (10 ng/ml), elevated sperm motility measured after 24 h storage (83.3% vs. 69.2% in control); the higher doses had no effect. IGF-I, given at 100 ng/ml, increased sperm fertilization rate (67.8% vs. 47.0%, p<0.05). In summary, IGF-I improved ram sperm functions during cooling storage and their effects were reflected in fertilizing ability in vitro. The study was aimed at examining the effects of IGF-I on ram sperm traits after hypothermic (4ºC) storage. Sperm ejaculates from 3 Lacaune rams were diluted in Triladyl, pooled and divided into the groups of IGF-I doses added (0, 10, 100 or 200 ng/ml). Following 72 h of storage the sperm samples were stained for a plasma membrane integrity (peanut agglutinin, PNA-FITC), membrane stability (annexin V-FITC) and apoptosis (Yo-Pro-1) and analyzed under a fluorescent microscope. Sperm motility was determined by CASA (at 0, 24 and 48h) and fertilizing ability (at 48h) - by an in vitro fertilization (IVF) test on bovine oocytes. IGF-I (100 and 200 ng/ml) decreased the portion of spermatozoa with disrupted plasma membrane integrity (15.9 and 16.5% resp. vs 28.3% in control, p< 0.05) and, given at all tested doses, reduced the rate of apoptotic sperm (6.2, 4.9 and 4.4% resp. vs 9.7% in control, p< 0.05). The proportion of spermatozoa with annexin V-labelled membrane changes was reduced by IGF-I from 9.6% (control) to 4.9% (100 ng/ml) and 6.1% (200 ng/ml). IGF-I (10 ng/ml), elevated sperm motility measured after 24 h storage (83.3% vs. 69.2% in control); the higher doses had no effect. IGF-I, given at 100 ng/ml, increased sperm fertilization rate (67.8% vs. 47.0%, p<0.05). In summary, IGF-I improved ram sperm functions during cooling storage and their effects were reflected in fertilizing ability in vitro.
dcterms:title
Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa
skos:prefLabel
Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa
skos:notation
RIV/49608851:_____/12:#0000581!RIV13-MZE-49608851
n8:predkladatel
n9:ico%3A49608851
n3:aktivita
n17:P
n3:aktivity
P(QH81324)
n3:cisloPeriodika
říjen 2012
n3:dodaniDat
n12:2013
n3:domaciTvurceVysledku
n4:9608230
n3:druhVysledku
n20:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
133097
n3:idVysledku
RIV/49608851:_____/12:#0000581
n3:jazykVysledku
n19:eng
n3:klicovaSlova
ram; sperm; IGF-I; viability; motility; fertilizing ability
n3:klicoveSlovo
n7:fertilizing%20ability n7:viability n7:sperm n7:IGF-I n7:motility n7:ram
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[71E140F00A66]
n3:nazevZdroje
Zygote
n3:obor
n16:GG
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:projekt
n18:QH81324
n3:rokUplatneniVysledku
n12:2012
n3:tvurceVysledku
Špaleková, Eliška Makarevič, Alexander Kubovičová, Elena Hegedüšová, Zdeňka Olexiková, Lucia
s:issn
0967-1994
s:numberOfPages
9
n14:doi
10.1017/S0967199412000500